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TH1/TH2 and TC1/TC2 profiles in peripheral blood and bronchoalveolar lavage fluid cells in pulmonary sarcoidosis.
J Allergy Clin Immunol. 2001 Feb; 107(2):337-44.JA

Abstract

BACKGROUND

Sarcoidosis is thought to be a type-1 cytokine-mediated disorder. However, few data are available on the profiles of cytokine expression by TH cells at the single-cell level, as assessed by intracellular cytokine flow cytometry. Additionally, it remains to be determined whether the balance of TC1 and TC2 cells can be altered in sarcoidosis.

OBJECTIVE

The aim of this study was to evaluate the TH1/TH2 and TC1/TC2 balances in sarcoidosis.

METHODS

Using triple-color flow cytometry and phorbol 12-myristate acetate/ionomycin stimulation, we measured the production of the intracellular cytokines IFN-gamma and IL-4 in CD4+ and CD8+ T cells separately, which were obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 patients with sarcoidosis, and compared their cytokine expressions with those of 10 normal subjects.

RESULTS

Under unstimulated conditions, there were no significant differences in the proportion of cytokine-producing CD4+ or CD8+ T cells in peripheral blood or BALF between patients with sarcoidosis and normal control subjects. On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. In contrast, no difference was found in the proportions of cytokine-producing CD8+ T cells or the ratio of IFN-gamma/IL-4-producing CD8+ T cells in either the peripheral blood or BALF between the patients and normal subjects.

CONCLUSIONS

These findings suggest that the prominent shift toward a type-1 phenotype may occur in CD4+ T-cell populations but not in CD8+ T-cell populations in the affected organs of sarcoidosis.

Authors+Show Affiliations

Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11174202

Citation

Inui, N, et al. "TH1/TH2 and TC1/TC2 Profiles in Peripheral Blood and Bronchoalveolar Lavage Fluid Cells in Pulmonary Sarcoidosis." The Journal of Allergy and Clinical Immunology, vol. 107, no. 2, 2001, pp. 337-44.
Inui N, Chida K, Suda T, et al. TH1/TH2 and TC1/TC2 profiles in peripheral blood and bronchoalveolar lavage fluid cells in pulmonary sarcoidosis. J Allergy Clin Immunol. 2001;107(2):337-44.
Inui, N., Chida, K., Suda, T., & Nakamura, H. (2001). TH1/TH2 and TC1/TC2 profiles in peripheral blood and bronchoalveolar lavage fluid cells in pulmonary sarcoidosis. The Journal of Allergy and Clinical Immunology, 107(2), 337-44.
Inui N, et al. TH1/TH2 and TC1/TC2 Profiles in Peripheral Blood and Bronchoalveolar Lavage Fluid Cells in Pulmonary Sarcoidosis. J Allergy Clin Immunol. 2001;107(2):337-44. PubMed PMID: 11174202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TH1/TH2 and TC1/TC2 profiles in peripheral blood and bronchoalveolar lavage fluid cells in pulmonary sarcoidosis. AU - Inui,N, AU - Chida,K, AU - Suda,T, AU - Nakamura,H, PY - 2001/2/15/pubmed PY - 2001/4/6/medline PY - 2001/2/15/entrez SP - 337 EP - 44 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 107 IS - 2 N2 - BACKGROUND: Sarcoidosis is thought to be a type-1 cytokine-mediated disorder. However, few data are available on the profiles of cytokine expression by TH cells at the single-cell level, as assessed by intracellular cytokine flow cytometry. Additionally, it remains to be determined whether the balance of TC1 and TC2 cells can be altered in sarcoidosis. OBJECTIVE: The aim of this study was to evaluate the TH1/TH2 and TC1/TC2 balances in sarcoidosis. METHODS: Using triple-color flow cytometry and phorbol 12-myristate acetate/ionomycin stimulation, we measured the production of the intracellular cytokines IFN-gamma and IL-4 in CD4+ and CD8+ T cells separately, which were obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 patients with sarcoidosis, and compared their cytokine expressions with those of 10 normal subjects. RESULTS: Under unstimulated conditions, there were no significant differences in the proportion of cytokine-producing CD4+ or CD8+ T cells in peripheral blood or BALF between patients with sarcoidosis and normal control subjects. On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. In contrast, no difference was found in the proportions of cytokine-producing CD8+ T cells or the ratio of IFN-gamma/IL-4-producing CD8+ T cells in either the peripheral blood or BALF between the patients and normal subjects. CONCLUSIONS: These findings suggest that the prominent shift toward a type-1 phenotype may occur in CD4+ T-cell populations but not in CD8+ T-cell populations in the affected organs of sarcoidosis. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/11174202/TH1/TH2_and_TC1/TC2_profiles_in_peripheral_blood_and_bronchoalveolar_lavage_fluid_cells_in_pulmonary_sarcoidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(01)17219-2 DB - PRIME DP - Unbound Medicine ER -