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5,10-methylenetetrahydrofolate reductase common mutations, folate status and plasma homocysteine in healthy French adults of the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) cohort.
Br J Nutr 2000; 84(6):891-6BJ

Abstract

The 677cytosine mutation identified in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene has been frequently associated with an elevated plasma homocysteine concentration. The aim of the present study was to determine the impact of this MTHFR common mutation on plasma and erythrocyte folate (RCF) and plasma total homocysteine (tHcy) concentrations in healthy French adults. A cohort of 291 subjects living in the Paris area and participating in the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) study were analysed to assess the impact of MTHFR polymorphism 677C-->T on folate status and plasma tHcy concentration. The frequency of the mutant homozygote for 677C-->T polymorphism (677TT genotype) in the present cohort was 16.8%. There were significant differences in plasma tHcy between 677CC, 677CT and 677TT genotype groups. The RCF concentrations were significantly different between each genotype, the lowest levels being associated with the 677TT genotype. When segregated by gender, no differences in tHcy between homozygous 677TT, heterozygous 677CT and wild-type 677CC genotype groups in women were observed. The fasting tHcy in women was unrelated to the 677C-->T mutation. However, tHcy was significantly increased in men with the homozygous 677TT genotype. We also analysed the possible implication of a second new MTHFR polymorphism (1298A-->C) in subjects with mild hyperhomocysteinaemia (4th quartile of homocysteinaemia; tHcy >11.1 micromol/l). The polymorphism 1298A-->C did not have a notable effect on tHcy or on the RCF levels. Our observations confirm a relatively high frequency of the 677TT genotype in the French population. Women with this genotype did not show the same increase in tHcy observed in men. In the present study dietary folate intake was not measured. Thus, the interaction of dietary folate with the MTHFR genotype in the French population needs further study.

Authors+Show Affiliations

Laboratoire de Biochimie Médicale et Pédiatrique, INSERM U-308, Faculté de Médecine, BP 184, F-54505 Vandoeuvre-lès-Nancy, France. jean-pierre.Nicolas@medicine.uhp-nancy.fr

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11177206

Citation

Chango, A, et al. "5,10-methylenetetrahydrofolate Reductase Common Mutations, Folate Status and Plasma Homocysteine in Healthy French Adults of the Supplementation En Vitamines Et Mineraux Antioxydants (SU.VI.MAX) Cohort." The British Journal of Nutrition, vol. 84, no. 6, 2000, pp. 891-6.
Chango A, Potier De Courcy G, Boisson F, et al. 5,10-methylenetetrahydrofolate reductase common mutations, folate status and plasma homocysteine in healthy French adults of the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) cohort. Br J Nutr. 2000;84(6):891-6.
Chango, A., Potier De Courcy, G., Boisson, F., Guilland, J. C., Barbé, F., Perrin, M. O., ... Nicolas, J. P. (2000). 5,10-methylenetetrahydrofolate reductase common mutations, folate status and plasma homocysteine in healthy French adults of the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) cohort. The British Journal of Nutrition, 84(6), pp. 891-6.
Chango A, et al. 5,10-methylenetetrahydrofolate Reductase Common Mutations, Folate Status and Plasma Homocysteine in Healthy French Adults of the Supplementation En Vitamines Et Mineraux Antioxydants (SU.VI.MAX) Cohort. Br J Nutr. 2000;84(6):891-6. PubMed PMID: 11177206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 5,10-methylenetetrahydrofolate reductase common mutations, folate status and plasma homocysteine in healthy French adults of the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) cohort. AU - Chango,A, AU - Potier De Courcy,G, AU - Boisson,F, AU - Guilland,J C, AU - Barbé,F, AU - Perrin,M O, AU - Christidès,J P, AU - Rabhi,K, AU - Pfister,M, AU - Galan,P, AU - Hercberg,S, AU - Nicolas,J P, PY - 2001/2/15/pubmed PY - 2001/3/7/medline PY - 2001/2/15/entrez SP - 891 EP - 6 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 84 IS - 6 N2 - The 677cytosine mutation identified in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene has been frequently associated with an elevated plasma homocysteine concentration. The aim of the present study was to determine the impact of this MTHFR common mutation on plasma and erythrocyte folate (RCF) and plasma total homocysteine (tHcy) concentrations in healthy French adults. A cohort of 291 subjects living in the Paris area and participating in the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) study were analysed to assess the impact of MTHFR polymorphism 677C-->T on folate status and plasma tHcy concentration. The frequency of the mutant homozygote for 677C-->T polymorphism (677TT genotype) in the present cohort was 16.8%. There were significant differences in plasma tHcy between 677CC, 677CT and 677TT genotype groups. The RCF concentrations were significantly different between each genotype, the lowest levels being associated with the 677TT genotype. When segregated by gender, no differences in tHcy between homozygous 677TT, heterozygous 677CT and wild-type 677CC genotype groups in women were observed. The fasting tHcy in women was unrelated to the 677C-->T mutation. However, tHcy was significantly increased in men with the homozygous 677TT genotype. We also analysed the possible implication of a second new MTHFR polymorphism (1298A-->C) in subjects with mild hyperhomocysteinaemia (4th quartile of homocysteinaemia; tHcy >11.1 micromol/l). The polymorphism 1298A-->C did not have a notable effect on tHcy or on the RCF levels. Our observations confirm a relatively high frequency of the 677TT genotype in the French population. Women with this genotype did not show the same increase in tHcy observed in men. In the present study dietary folate intake was not measured. Thus, the interaction of dietary folate with the MTHFR genotype in the French population needs further study. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/11177206/510_methylenetetrahydrofolate_reductase_common_mutations_folate_status_and_plasma_homocysteine_in_healthy_French_adults_of_the_Supplementation_en_Vitamines_et_Mineraux_Antioxydants__SU_VI_MAX__cohort_ L2 - https://www.cambridge.org/core/product/identifier/S0007114500002683/type/journal_article DB - PRIME DP - Unbound Medicine ER -