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PCR and in situ hybridization studies of telomerase subunits in human non-neoplastic livers.
J Pathol. 2001 Feb; 193(2):210-7.JP

Abstract

Telomerase, a ribonucleoprotein enzyme associated with cellular immortality, consists of human telomerase RNA component (hTERC), human telomerase protein 1 (hTEP1), and human telomerase reverse transcriptase (hTERT). In this study, the expression of these subunits was examined in non-neoplastic livers [13 cases of chronic viral hepatitis (CVH), 16 of primary biliary cirrhosis (PBC), two of primary sclerosing cholangitis, and six normal livers], using the reverse transcription-polymerase chain reaction (RT-PCR), nested PCR, and in situ hybridization (ISH). Six hepatocellular carcinoma (HCC) cases and one colonic cancer were used as positive controls. Telomeric repeat amplification protocol (TRAP) assay disclosed distinct telomerase activity in all positive controls and weak telomerase activity in non-neoplastic livers in 4 of 13 CVH cases and 5 of 16 PBC cases. By RT- and nested PCR, both hTERC and hTEP1 mRNA were detectable in all non-neoplastic liver tissues; ISH revealed hTERC and hTEP1 mRNA in the periportal and periseptal hepatocytes and inflammatory mononuclear cells in those cases examined. ISH revealed hTERT mRNA only in a few infiltrating mononuclear cells in 3 of 13 CVH and 2 of 16 PBC livers and these five cases were also positive by TRAP assay. In four of these five cases, hTERT mRNA was also detectable by nested PCR, suggesting that hTERT mRNA in the non-neoplastic liver is expressed by infiltrating mononuclear cells. Biliary epithelial cells were totally negative for these human telomerase subunits. Three subunits were constantly detected in all positive controls by ISH as well as by RT- and nested PCR. The finding that hTERC and hTEP1 mRNA, but not hTERT mRNA, were detectable in the non-neoplastic hepatocytes suggests that telomerase is present but not activated and that additional factor(s) are necessary for the expression of hTERT mRNA in the hepatocytes, along with immortalization and neoplastic transformation.

Authors+Show Affiliations

Department of Pathology (II), Kanazawa University School of Medicine, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11180168

Citation

Harada, K, et al. "PCR and in Situ Hybridization Studies of Telomerase Subunits in Human Non-neoplastic Livers." The Journal of Pathology, vol. 193, no. 2, 2001, pp. 210-7.
Harada K, Yasoshima M, Ozaki S, et al. PCR and in situ hybridization studies of telomerase subunits in human non-neoplastic livers. J Pathol. 2001;193(2):210-7.
Harada, K., Yasoshima, M., Ozaki, S., Sanzen, T., & Nakanuma, Y. (2001). PCR and in situ hybridization studies of telomerase subunits in human non-neoplastic livers. The Journal of Pathology, 193(2), 210-7.
Harada K, et al. PCR and in Situ Hybridization Studies of Telomerase Subunits in Human Non-neoplastic Livers. J Pathol. 2001;193(2):210-7. PubMed PMID: 11180168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PCR and in situ hybridization studies of telomerase subunits in human non-neoplastic livers. AU - Harada,K, AU - Yasoshima,M, AU - Ozaki,S, AU - Sanzen,T, AU - Nakanuma,Y, PY - 2001/2/17/pubmed PY - 2001/3/27/medline PY - 2001/2/17/entrez SP - 210 EP - 7 JF - The Journal of pathology JO - J Pathol VL - 193 IS - 2 N2 - Telomerase, a ribonucleoprotein enzyme associated with cellular immortality, consists of human telomerase RNA component (hTERC), human telomerase protein 1 (hTEP1), and human telomerase reverse transcriptase (hTERT). In this study, the expression of these subunits was examined in non-neoplastic livers [13 cases of chronic viral hepatitis (CVH), 16 of primary biliary cirrhosis (PBC), two of primary sclerosing cholangitis, and six normal livers], using the reverse transcription-polymerase chain reaction (RT-PCR), nested PCR, and in situ hybridization (ISH). Six hepatocellular carcinoma (HCC) cases and one colonic cancer were used as positive controls. Telomeric repeat amplification protocol (TRAP) assay disclosed distinct telomerase activity in all positive controls and weak telomerase activity in non-neoplastic livers in 4 of 13 CVH cases and 5 of 16 PBC cases. By RT- and nested PCR, both hTERC and hTEP1 mRNA were detectable in all non-neoplastic liver tissues; ISH revealed hTERC and hTEP1 mRNA in the periportal and periseptal hepatocytes and inflammatory mononuclear cells in those cases examined. ISH revealed hTERT mRNA only in a few infiltrating mononuclear cells in 3 of 13 CVH and 2 of 16 PBC livers and these five cases were also positive by TRAP assay. In four of these five cases, hTERT mRNA was also detectable by nested PCR, suggesting that hTERT mRNA in the non-neoplastic liver is expressed by infiltrating mononuclear cells. Biliary epithelial cells were totally negative for these human telomerase subunits. Three subunits were constantly detected in all positive controls by ISH as well as by RT- and nested PCR. The finding that hTERC and hTEP1 mRNA, but not hTERT mRNA, were detectable in the non-neoplastic hepatocytes suggests that telomerase is present but not activated and that additional factor(s) are necessary for the expression of hTERT mRNA in the hepatocytes, along with immortalization and neoplastic transformation. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/11180168/PCR_and_in_situ_hybridization_studies_of_telomerase_subunits_in_human_non_neoplastic_livers_ L2 - https://doi.org/10.1002/1096-9896(2000)9999:9999<::AID-PATH786>3.0.CO;2-G DB - PRIME DP - Unbound Medicine ER -