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Acute effects of peritoneal dialysis with dialysates containing dextrose or dextrose and amino acids on muscle protein turnover in patients with chronic renal failure.
J Am Soc Nephrol. 2001 Mar; 12(3):557-67.JA

Abstract

Whether changes in substrate and insulin levels that occur during peritoneal dialysis (PD) have effects on muscle protein dynamics was evaluated by studying muscle protein synthesis (PS), breakdown (PB), and net protein balance (NB) by the forearm perfusion method associated with the kinetics of 3H-phenylalanine in acute, crossover studies in which PD patients served as their own controls. Studies were performed (1) in the basal state and during PD with dialysates that contained dextrose alone in different concentrations (protocol 1: eight patients), (2) during PD with dialysates that contained dextrose alone or dextrose and amino acids (AA) (protocol 2: five patients), and (3) in time controls (five patients). PD with dextrose alone induced (1) a two- to threefold increase in insulin, as well as a 20 to 25% decrease in AA, mainly BCAA, levels; (2) an insulin-related decline (-18%) in forearm PB (P<0.002); (3) a 20% decrease in muscle PS (P<0.04), which was related to arterial BCAA and K+ (P<0.02 to 0.05); (4) a persistent negative NB; and (5) a decrease in the efficiency of muscle protein turnover, expressed as the ratio NB/PB. PD with dextrose+AA versus PD with dextrose induced (1) similarly high insulin levels but with a significant increase in total arterial AA (+30 to 110%), mainly valine; (2) a reduced release of AA from muscle (P<0.05); and (3) a decrease in the negative NB observed during PD with dextrose, owing to an increase (approximately 20%) in muscle PS, without any further effect on muscle PB. This study indicates that in PD patients in the fasting state, the moderate hyperinsulinemia that occurs during PD with dextrose alone causes an antiproteolytic action that is obscured by a parallel decrease in AA availability for PS. Conversely, the combined use of dextrose and AA results in a cumulative effect, because of the suppression of endogenous muscle PB (induced by insulin) and the stimulation of muscle PS (induced by AA availability). The hypothesis, therefore, is that in patients who are treated with PD, when fasting or when nutrient intake is reduced, muscle mass could be maintained better by the combined use of dextrose and AA.

Authors+Show Affiliations

Department of Internal Medicine, Division of Nephrology, University of Genoa, Genoa, Italy. gari@unige.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11181804

Citation

Garibotto, G, et al. "Acute Effects of Peritoneal Dialysis With Dialysates Containing Dextrose or Dextrose and Amino Acids On Muscle Protein Turnover in Patients With Chronic Renal Failure." Journal of the American Society of Nephrology : JASN, vol. 12, no. 3, 2001, pp. 557-67.
Garibotto G, Sofia A, Canepa A, et al. Acute effects of peritoneal dialysis with dialysates containing dextrose or dextrose and amino acids on muscle protein turnover in patients with chronic renal failure. J Am Soc Nephrol. 2001;12(3):557-67.
Garibotto, G., Sofia, A., Canepa, A., Saffioti, S., Sacco, P., Sala, M., Dertenois, L., Pastorino, N., Deferrari, G., & Russo, R. (2001). Acute effects of peritoneal dialysis with dialysates containing dextrose or dextrose and amino acids on muscle protein turnover in patients with chronic renal failure. Journal of the American Society of Nephrology : JASN, 12(3), 557-67.
Garibotto G, et al. Acute Effects of Peritoneal Dialysis With Dialysates Containing Dextrose or Dextrose and Amino Acids On Muscle Protein Turnover in Patients With Chronic Renal Failure. J Am Soc Nephrol. 2001;12(3):557-67. PubMed PMID: 11181804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute effects of peritoneal dialysis with dialysates containing dextrose or dextrose and amino acids on muscle protein turnover in patients with chronic renal failure. AU - Garibotto,G, AU - Sofia,A, AU - Canepa,A, AU - Saffioti,S, AU - Sacco,P, AU - Sala,M, AU - Dertenois,L, AU - Pastorino,N, AU - Deferrari,G, AU - Russo,R, PY - 2001/2/22/pubmed PY - 2001/5/25/medline PY - 2001/2/22/entrez SP - 557 EP - 67 JF - Journal of the American Society of Nephrology : JASN JO - J. Am. Soc. Nephrol. VL - 12 IS - 3 N2 - Whether changes in substrate and insulin levels that occur during peritoneal dialysis (PD) have effects on muscle protein dynamics was evaluated by studying muscle protein synthesis (PS), breakdown (PB), and net protein balance (NB) by the forearm perfusion method associated with the kinetics of 3H-phenylalanine in acute, crossover studies in which PD patients served as their own controls. Studies were performed (1) in the basal state and during PD with dialysates that contained dextrose alone in different concentrations (protocol 1: eight patients), (2) during PD with dialysates that contained dextrose alone or dextrose and amino acids (AA) (protocol 2: five patients), and (3) in time controls (five patients). PD with dextrose alone induced (1) a two- to threefold increase in insulin, as well as a 20 to 25% decrease in AA, mainly BCAA, levels; (2) an insulin-related decline (-18%) in forearm PB (P<0.002); (3) a 20% decrease in muscle PS (P<0.04), which was related to arterial BCAA and K+ (P<0.02 to 0.05); (4) a persistent negative NB; and (5) a decrease in the efficiency of muscle protein turnover, expressed as the ratio NB/PB. PD with dextrose+AA versus PD with dextrose induced (1) similarly high insulin levels but with a significant increase in total arterial AA (+30 to 110%), mainly valine; (2) a reduced release of AA from muscle (P<0.05); and (3) a decrease in the negative NB observed during PD with dextrose, owing to an increase (approximately 20%) in muscle PS, without any further effect on muscle PB. This study indicates that in PD patients in the fasting state, the moderate hyperinsulinemia that occurs during PD with dextrose alone causes an antiproteolytic action that is obscured by a parallel decrease in AA availability for PS. Conversely, the combined use of dextrose and AA results in a cumulative effect, because of the suppression of endogenous muscle PB (induced by insulin) and the stimulation of muscle PS (induced by AA availability). The hypothesis, therefore, is that in patients who are treated with PD, when fasting or when nutrient intake is reduced, muscle mass could be maintained better by the combined use of dextrose and AA. SN - 1046-6673 UR - https://www.unboundmedicine.com/medline/citation/11181804/Acute_effects_of_peritoneal_dialysis_with_dialysates_containing_dextrose_or_dextrose_and_amino_acids_on_muscle_protein_turnover_in_patients_with_chronic_renal_failure_ L2 - http://jasn.asnjournals.org/cgi/pmidlookup?view=long&amp;pmid=11181804 DB - PRIME DP - Unbound Medicine ER -