Tags

Type your tag names separated by a space and hit enter

[Cloning and expression of the E. coli serine hydroxymethyltransferase gene (glyA)].
Wei Sheng Wu Xue Bao. 1997 Dec; 37(6):423-8.WS

Abstract

The E. coli K12 glyA gene(13 kb), encoding serine hydroxymethyltransferase (SHMT), has been cloned in the plasmid vector pBR329 using insertion inactivation and complementation test. Subcloning of segments of the original insert (13 kb) into plasmids pBR322, pBR329 and pSMY901 established that a 2.6 kb PstI-EcoR fragment carries the glyA gene. The 12 strains of transforments containing recombined plasmid. were obtained. SHMT and glyA gene product level in strains carrying glyA plasmids were different. No SHMT activity was observed in host strains. The glyA gene products for JM109(pSM13), K12(pSM13), K12(pSM14) and K12(pSM15) account for 15.7%, 15.4%, 11.8%, and 9.48% of the total dissoluble cell protein, respectively.

Authors+Show Affiliations

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

11189372

Citation

Shen, T, et al. "[Cloning and Expression of the E. Coli Serine Hydroxymethyltransferase Gene (glyA)]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 37, no. 6, 1997, pp. 423-8.
Shen T, Na S, Yu G, et al. [Cloning and expression of the E. coli serine hydroxymethyltransferase gene (glyA)]. Wei Sheng Wu Xue Bao. 1997;37(6):423-8.
Shen, T., Na, S., Yu, G., Xie, J., & Jia, P. (1997). [Cloning and expression of the E. coli serine hydroxymethyltransferase gene (glyA)]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 37(6), 423-8.
Shen T, et al. [Cloning and Expression of the E. Coli Serine Hydroxymethyltransferase Gene (glyA)]. Wei Sheng Wu Xue Bao. 1997;37(6):423-8. PubMed PMID: 11189372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cloning and expression of the E. coli serine hydroxymethyltransferase gene (glyA)]. AU - Shen,T, AU - Na,S, AU - Yu,G, AU - Xie,J, AU - Jia,P, PY - 2001/2/24/pubmed PY - 2001/3/3/medline PY - 2001/2/24/entrez SP - 423 EP - 8 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 37 IS - 6 N2 - The E. coli K12 glyA gene(13 kb), encoding serine hydroxymethyltransferase (SHMT), has been cloned in the plasmid vector pBR329 using insertion inactivation and complementation test. Subcloning of segments of the original insert (13 kb) into plasmids pBR322, pBR329 and pSMY901 established that a 2.6 kb PstI-EcoR fragment carries the glyA gene. The 12 strains of transforments containing recombined plasmid. were obtained. SHMT and glyA gene product level in strains carrying glyA plasmids were different. No SHMT activity was observed in host strains. The glyA gene products for JM109(pSM13), K12(pSM13), K12(pSM14) and K12(pSM15) account for 15.7%, 15.4%, 11.8%, and 9.48% of the total dissoluble cell protein, respectively. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/11189372/[Cloning_and_expression_of_the_E__coli_serine_hydroxymethyltransferase_gene__glyA_]_ DB - PRIME DP - Unbound Medicine ER -