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Chiral analysis of baclofen by alpha-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection.
Electrophoresis. 2001 Jan; 22(1):123-7.E

Abstract

An enantioselective method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoresis (CE) separation and laser-induced fluorescence (LIF) detection has been developed. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of the nonfluorescent drug. alpha-Cyclodextrin (alpha-CD) was included in the buffer as a chiral selector for the separation of NDA-labeled S-(+)- and R-(-)-baclofen. Optimal resolution and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) containing 7 mM alpha-CD and a He-Cd laser (lambda ex = 442 nm, lambda em = 500 nm). Combined with a simple cleanup procedure, this method can be applied to the analysis of baclofen enantiomers in human plasma. The relative standard deviation (RSD) values on peak areas of a plasma sample containing 1.0 microM racemic baclofen were 6.4 and 4.9% (n = 8) for the S-(+)- and R-(-)-enantiomer, respectively. The RSD value on migration times of both enantiomers was 0.5% (n = 8). Calibration graphs for S-(+)- and R-(-)-baclofen in plasma showed a good linearity (r > or = 0.999) in the concentration range of 0.1-2.0 microM. The limit of detection of baclofen in plasma was about 10 ng/mL.

Authors+Show Affiliations

Department of Chemistry, Tunghai University, Taichung, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11197160

Citation

Chiang, M T., et al. "Chiral Analysis of Baclofen By Alpha-cyclodextrin-modified Capillary Electrophoresis and Laser-induced Fluorescence Detection." Electrophoresis, vol. 22, no. 1, 2001, pp. 123-7.
Chiang MT, Chang SY, Whang CW. Chiral analysis of baclofen by alpha-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection. Electrophoresis. 2001;22(1):123-7.
Chiang, M. T., Chang, S. Y., & Whang, C. W. (2001). Chiral analysis of baclofen by alpha-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection. Electrophoresis, 22(1), 123-7.
Chiang MT, Chang SY, Whang CW. Chiral Analysis of Baclofen By Alpha-cyclodextrin-modified Capillary Electrophoresis and Laser-induced Fluorescence Detection. Electrophoresis. 2001;22(1):123-7. PubMed PMID: 11197160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chiral analysis of baclofen by alpha-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection. AU - Chiang,M T, AU - Chang,S Y, AU - Whang,C W, PY - 2001/2/24/pubmed PY - 2001/5/1/medline PY - 2001/2/24/entrez SP - 123 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 1 N2 - An enantioselective method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoresis (CE) separation and laser-induced fluorescence (LIF) detection has been developed. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of the nonfluorescent drug. alpha-Cyclodextrin (alpha-CD) was included in the buffer as a chiral selector for the separation of NDA-labeled S-(+)- and R-(-)-baclofen. Optimal resolution and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) containing 7 mM alpha-CD and a He-Cd laser (lambda ex = 442 nm, lambda em = 500 nm). Combined with a simple cleanup procedure, this method can be applied to the analysis of baclofen enantiomers in human plasma. The relative standard deviation (RSD) values on peak areas of a plasma sample containing 1.0 microM racemic baclofen were 6.4 and 4.9% (n = 8) for the S-(+)- and R-(-)-enantiomer, respectively. The RSD value on migration times of both enantiomers was 0.5% (n = 8). Calibration graphs for S-(+)- and R-(-)-baclofen in plasma showed a good linearity (r > or = 0.999) in the concentration range of 0.1-2.0 microM. The limit of detection of baclofen in plasma was about 10 ng/mL. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11197160/Chiral_analysis_of_baclofen_by_alpha_cyclodextrin_modified_capillary_electrophoresis_and_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -