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Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection.
Electrophoresis. 2001 Jan; 22(1):128-33.E

Abstract

Capillary zone electrophoresis was employed for the determination of human serum transferrin using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.9 V vs. saturated calomel electrode (SCE). The optimum conditions of separation and detection are 7.5 x 10(-4) mol/L Tris-3.44 x 10(-4) mol/L HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 6.7 x 10(-8) mol/L or 440 amol (S/N = 2). The relative standard deviations are 0.67% for the migration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of transferrin in human serum. The recovery is between 93-104%.

Authors+Show Affiliations

Dong Q
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China.
Yu D
No affiliation info available
Ye X
No affiliation info available
Jin W
No affiliation info available

MeSH

AdultElectrophoresis, CapillaryHumansTransferrin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11197161

Citation

Dong, Q, et al. "Monitoring Human Serum Transferrin By Capillary Zone Electrophoresis With End-column Amperometric Detection." Electrophoresis, vol. 22, no. 1, 2001, pp. 128-33.
Dong Q, Yu D, Ye X, et al. Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis. 2001;22(1):128-33.
Dong, Q., Yu, D., Ye, X., & Jin, W. (2001). Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection. Electrophoresis, 22(1), 128-33.
Dong Q, et al. Monitoring Human Serum Transferrin By Capillary Zone Electrophoresis With End-column Amperometric Detection. Electrophoresis. 2001;22(1):128-33. PubMed PMID: 11197161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection. AU - Dong,Q, AU - Yu,D, AU - Ye,X, AU - Jin,W, PY - 2001/2/24/pubmed PY - 2001/5/1/medline PY - 2001/2/24/entrez SP - 128 EP - 33 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 1 N2 - Capillary zone electrophoresis was employed for the determination of human serum transferrin using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.9 V vs. saturated calomel electrode (SCE). The optimum conditions of separation and detection are 7.5 x 10(-4) mol/L Tris-3.44 x 10(-4) mol/L HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 6.7 x 10(-8) mol/L or 440 amol (S/N = 2). The relative standard deviations are 0.67% for the migration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of transferrin in human serum. The recovery is between 93-104%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11197161/Monitoring_human_serum_transferrin_by_capillary_zone_electrophoresis_with_end_column_amperometric_detection_ DB - PRIME DP - Unbound Medicine ER -
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