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Determination of synthesized alpha-vitamin E by micellar electrokinetic chromatography.
Electrophoresis. 2001 Jan; 22(1):151-4.E

Abstract

We developed a micellar electrokinetic chromatography method (MEKC) for the direct determination of the content of synthesized alpha-vitamin E. It was found that under the optimum separation conditions 7 mM borate + 14 mM phosphate + 15 mM sodium dodecyl sulfate (SDS) + 10 mM sodium cholate (NaCh) + 8% acetonitrile (pH 9.2) with UV detection wavelength at 214 nm, 16 kV constant voltage, and 26 degrees C constant temperature, alpha-vitamin E and its isomers can be baseline separated and alpha-vitamin E was quantitatively analyzed. In addition, the sample recovery, the limit of detection and the repeatability of the method were investigated. The influence of various parameters on the separation such as SDS concentration, NaCh concentration, buffer pH and acetonitrile percentage were also discussed.

Authors+Show Affiliations

Zhao J
Department of Chemistry, Hebei University, Baoding 071002, China.
Yang G
No affiliation info available
Duan H
No affiliation info available
Li J
No affiliation info available

MeSH

AcetonitrilesChromatography, Micellar Electrokinetic CapillaryHydrogen-Ion ConcentrationMolecular StructureSodium CholateSodium Dodecyl SulfateVitamin E

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11197165

Citation

Zhao, J, et al. "Determination of Synthesized Alpha-vitamin E By Micellar Electrokinetic Chromatography." Electrophoresis, vol. 22, no. 1, 2001, pp. 151-4.
Zhao J, Yang G, Duan H, et al. Determination of synthesized alpha-vitamin E by micellar electrokinetic chromatography. Electrophoresis. 2001;22(1):151-4.
Zhao, J., Yang, G., Duan, H., & Li, J. (2001). Determination of synthesized alpha-vitamin E by micellar electrokinetic chromatography. Electrophoresis, 22(1), 151-4.
Zhao J, et al. Determination of Synthesized Alpha-vitamin E By Micellar Electrokinetic Chromatography. Electrophoresis. 2001;22(1):151-4. PubMed PMID: 11197165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of synthesized alpha-vitamin E by micellar electrokinetic chromatography. AU - Zhao,J, AU - Yang,G, AU - Duan,H, AU - Li,J, PY - 2001/2/24/pubmed PY - 2001/5/1/medline PY - 2001/2/24/entrez SP - 151 EP - 4 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 1 N2 - We developed a micellar electrokinetic chromatography method (MEKC) for the direct determination of the content of synthesized alpha-vitamin E. It was found that under the optimum separation conditions 7 mM borate + 14 mM phosphate + 15 mM sodium dodecyl sulfate (SDS) + 10 mM sodium cholate (NaCh) + 8% acetonitrile (pH 9.2) with UV detection wavelength at 214 nm, 16 kV constant voltage, and 26 degrees C constant temperature, alpha-vitamin E and its isomers can be baseline separated and alpha-vitamin E was quantitatively analyzed. In addition, the sample recovery, the limit of detection and the repeatability of the method were investigated. The influence of various parameters on the separation such as SDS concentration, NaCh concentration, buffer pH and acetonitrile percentage were also discussed. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11197165/Determination_of_synthesized_alpha_vitamin_E_by_micellar_electrokinetic_chromatography_ DB - PRIME DP - Unbound Medicine ER -