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In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics.
J Endod. 2000 Jul; 26(7):391-4.JE

Abstract

The antimicrobial activity of four root canal sealers (AH Plus, Sealapex, Ketac Endo, and Fill Canal), two calcium hydroxide pastes (Calen and Calasept), and a zinc oxide paste was evaluated. Seven bacterial strains were used, six of them standard; Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, and Enterococcus faecalis ATCC 10541. There was a wild strain of Streptococcus mutans isolated from saliva obtained in an adult dental clinic. Activity was evaluated using the agar diffusion method with Brain Heart Infusion agar and Müller Hinton medium seeded by pour plate. Calcium hydroxide-based sealers and pastes were either placed directly into 4.0 x 4.0 mm wells or by using absorbent paper points. The plates were kept at room temperature for 2 hr for diffusion. After incubation at 37 degrees C for 24 hr, the medium was optimized with 0.05 g% TTC gel and inhibition haloes were measured. All bacterial strains were inhibited by all materials using the well method. However, when the materials were applied with absorbent paper points, Enterococcus faecalis was not inhibited by zinc oxide, and Pseudomonas aeruginosa was not inhibited by AH Plus, Fill Canal, and the zinc oxide-based paste. We conclude that sealers and pastes presented antimicrobial activity in vitro and culture medium optimization with 0.05 g% TTC gel facilitated observation of the inhibition haloes.

Authors+Show Affiliations

Departamento de Endodontia, Faculdade de Odontologia de Araraquara-UNESP, Caixa Postal 331, Rua Humaitá, 1680, 14801-903 Araraquara, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11199762

Citation

Leonardo, M R., et al. "In Vitro Evaluation of Antimicrobial Activity of Sealers and Pastes Used in Endodontics." Journal of Endodontics, vol. 26, no. 7, 2000, pp. 391-4.
Leonardo MR, da Silva LA, Tanomaru Filho M, et al. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. J Endod. 2000;26(7):391-4.
Leonardo, M. R., da Silva, L. A., Tanomaru Filho, M., Bonifácio, K. C., & Ito, I. Y. (2000). In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. Journal of Endodontics, 26(7), 391-4.
Leonardo MR, et al. In Vitro Evaluation of Antimicrobial Activity of Sealers and Pastes Used in Endodontics. J Endod. 2000;26(7):391-4. PubMed PMID: 11199762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. AU - Leonardo,M R, AU - da Silva,L A, AU - Tanomaru Filho,M, AU - Bonifácio,K C, AU - Ito,I Y, PY - 2001/2/24/pubmed PY - 2001/3/7/medline PY - 2001/2/24/entrez SP - 391 EP - 4 JF - Journal of endodontics JO - J Endod VL - 26 IS - 7 N2 - The antimicrobial activity of four root canal sealers (AH Plus, Sealapex, Ketac Endo, and Fill Canal), two calcium hydroxide pastes (Calen and Calasept), and a zinc oxide paste was evaluated. Seven bacterial strains were used, six of them standard; Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, and Enterococcus faecalis ATCC 10541. There was a wild strain of Streptococcus mutans isolated from saliva obtained in an adult dental clinic. Activity was evaluated using the agar diffusion method with Brain Heart Infusion agar and Müller Hinton medium seeded by pour plate. Calcium hydroxide-based sealers and pastes were either placed directly into 4.0 x 4.0 mm wells or by using absorbent paper points. The plates were kept at room temperature for 2 hr for diffusion. After incubation at 37 degrees C for 24 hr, the medium was optimized with 0.05 g% TTC gel and inhibition haloes were measured. All bacterial strains were inhibited by all materials using the well method. However, when the materials were applied with absorbent paper points, Enterococcus faecalis was not inhibited by zinc oxide, and Pseudomonas aeruginosa was not inhibited by AH Plus, Fill Canal, and the zinc oxide-based paste. We conclude that sealers and pastes presented antimicrobial activity in vitro and culture medium optimization with 0.05 g% TTC gel facilitated observation of the inhibition haloes. SN - 0099-2399 UR - https://www.unboundmedicine.com/medline/citation/11199762/In_vitro_evaluation_of_antimicrobial_activity_of_sealers_and_pastes_used_in_endodontics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0099-2399(05)60901-6 DB - PRIME DP - Unbound Medicine ER -