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NF-kappa B does not modulate sensitivity of renal carcinoma cells to TNF alpha-related apoptosis-inducing ligand (TRAIL).
Anticancer Res. 2000 Nov-Dec; 20(6B):4243-55.AR

Abstract

BACKGROUND

Immune cells express TNF-related apoptosis-inducing ligand (TRAIL) and may play an important role in controlling the growth of renal cancer. For this reason we examined the potential cytotoxic and intracellular signaling effects of TRAIL on several renal cancer cell (RCC) lines.

METHODS

Cytotoxicity was determined using an MTS tetrazolium based assay. Death receptor expression was measured by reverse-transcription polymerase chain reaction. Immunoblot analysis was used to measure cFLIP, caspase-8 and phosphorylated JNK and p38. Activation of NF-kappa B was determined by electrophoretic mobility shift assays.

RESULTS

Two RCC lines were highly sensitive to TRAIL-induced cell death, two were of intermediate sensitivity, and two were insensitive. Sensitivity to TRAIL-stimulated apoptosis did not correlate with expression of death receptors, decoy receptors, or expression of the caspase-8-inhibitory protein cFLIP. Neither activation or inhibition of the NF-kappa B signal transduction pathway protected sensitive cells from TRAIL-induced cell death.

CONCLUSIONS

NF-kappa B, although stimulated in these cell lines, does not play a role in the sensitivity of RCC to TRAIL and could play other functions in TRAIL signaling.

Authors+Show Affiliations

Division of Medical Oncology, School of Medicine, University of Colorado Health Sciences Center, 4200 East Ninth Ave., Denver, CO 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11205254

Citation

Pawlowski, J E., et al. "NF-kappa B Does Not Modulate Sensitivity of Renal Carcinoma Cells to TNF Alpha-related Apoptosis-inducing Ligand (TRAIL)." Anticancer Research, vol. 20, no. 6B, 2000, pp. 4243-55.
Pawlowski JE, Nesterov A, Scheinman RI, et al. NF-kappa B does not modulate sensitivity of renal carcinoma cells to TNF alpha-related apoptosis-inducing ligand (TRAIL). Anticancer Res. 2000;20(6B):4243-55.
Pawlowski, J. E., Nesterov, A., Scheinman, R. I., Johnson, T. R., & Kraft, A. S. (2000). NF-kappa B does not modulate sensitivity of renal carcinoma cells to TNF alpha-related apoptosis-inducing ligand (TRAIL). Anticancer Research, 20(6B), 4243-55.
Pawlowski JE, et al. NF-kappa B Does Not Modulate Sensitivity of Renal Carcinoma Cells to TNF Alpha-related Apoptosis-inducing Ligand (TRAIL). Anticancer Res. 2000 Nov-Dec;20(6B):4243-55. PubMed PMID: 11205254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NF-kappa B does not modulate sensitivity of renal carcinoma cells to TNF alpha-related apoptosis-inducing ligand (TRAIL). AU - Pawlowski,J E, AU - Nesterov,A, AU - Scheinman,R I, AU - Johnson,T R, AU - Kraft,A S, PY - 2001/2/24/pubmed PY - 2001/3/7/medline PY - 2001/2/24/entrez SP - 4243 EP - 55 JF - Anticancer research JO - Anticancer Res VL - 20 IS - 6B N2 - BACKGROUND: Immune cells express TNF-related apoptosis-inducing ligand (TRAIL) and may play an important role in controlling the growth of renal cancer. For this reason we examined the potential cytotoxic and intracellular signaling effects of TRAIL on several renal cancer cell (RCC) lines. METHODS: Cytotoxicity was determined using an MTS tetrazolium based assay. Death receptor expression was measured by reverse-transcription polymerase chain reaction. Immunoblot analysis was used to measure cFLIP, caspase-8 and phosphorylated JNK and p38. Activation of NF-kappa B was determined by electrophoretic mobility shift assays. RESULTS: Two RCC lines were highly sensitive to TRAIL-induced cell death, two were of intermediate sensitivity, and two were insensitive. Sensitivity to TRAIL-stimulated apoptosis did not correlate with expression of death receptors, decoy receptors, or expression of the caspase-8-inhibitory protein cFLIP. Neither activation or inhibition of the NF-kappa B signal transduction pathway protected sensitive cells from TRAIL-induced cell death. CONCLUSIONS: NF-kappa B, although stimulated in these cell lines, does not play a role in the sensitivity of RCC to TRAIL and could play other functions in TRAIL signaling. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/11205254/NF_kappa_B_does_not_modulate_sensitivity_of_renal_carcinoma_cells_to_TNF_alpha_related_apoptosis_inducing_ligand__TRAIL__ L2 - https://medlineplus.gov/kidneycancer.html DB - PRIME DP - Unbound Medicine ER -