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Mechanism of induction of transforming growth factor-beta type II receptor gene expression by v-Src in murine myeloid cells.
Cell Growth Differ. 2001 Jan; 12(1):9-18.CG

Abstract

Transforming growth factor (TGF)-beta1 plays an important role during hematopoiesis. Previously, we had shown that the growth of a v-Src-transformed myeloid cell line was markedly more inhibited by TGF-beta treatment when compared with the wild-type myeloid cell line. To investigate the increased growth sensitivity of the v-Src-transformed myeloid cell line, 32D-src, to TGF-beta, we examined expression of the TGF-beta type II receptor (TGF-beta RII) gene in myeloid cell lines. Northem blot analysis showed that expression of approximately 8- and 6-kb species of TGF-beta RII transcripts was markedly increased in the 32D-src cell line. The expression of the TGF-beta RII promoter linked to a reporter gene was increased 23-fold by v-Src. DNA transfection and electrophoretic mobility shift assay revealed that v-Src induces TGF-beta RII promoter activity through an AP1/ATF2-like sequence (-219 to -172), ETS binding sites (+1 to +36), and the inverted CCAAT box (-81 to -77). Novel DNA-protein complexes with ETS binding sites are significantly increased in v-src-transformed cell lines compared with the control cell line. These results suggest that v-Src induces activity of the TGF-beta RII promoter through multiple elements by inducing expression of nuclear proteins interacting with these elements.

Authors+Show Affiliations

Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892-5055, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11205746

Citation

Park, S H., et al. "Mechanism of Induction of Transforming Growth Factor-beta Type II Receptor Gene Expression By v-Src in Murine Myeloid Cells." Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research, vol. 12, no. 1, 2001, pp. 9-18.
Park SH, Birchenall-Roberts MC, Yi Y, et al. Mechanism of induction of transforming growth factor-beta type II receptor gene expression by v-Src in murine myeloid cells. Cell Growth Differ. 2001;12(1):9-18.
Park, S. H., Birchenall-Roberts, M. C., Yi, Y., Lee, B. I., Lee, D. K., Bertolette, D. C., Fu, T., Ruscetti, F., & Kim, S. J. (2001). Mechanism of induction of transforming growth factor-beta type II receptor gene expression by v-Src in murine myeloid cells. Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research, 12(1), 9-18.
Park SH, et al. Mechanism of Induction of Transforming Growth Factor-beta Type II Receptor Gene Expression By v-Src in Murine Myeloid Cells. Cell Growth Differ. 2001;12(1):9-18. PubMed PMID: 11205746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of induction of transforming growth factor-beta type II receptor gene expression by v-Src in murine myeloid cells. AU - Park,S H, AU - Birchenall-Roberts,M C, AU - Yi,Y, AU - Lee,B I, AU - Lee,D K, AU - Bertolette,D C, AU - Fu,T, AU - Ruscetti,F, AU - Kim,S J, PY - 2001/2/24/pubmed PY - 2001/4/3/medline PY - 2001/2/24/entrez SP - 9 EP - 18 JF - Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research JO - Cell Growth Differ VL - 12 IS - 1 N2 - Transforming growth factor (TGF)-beta1 plays an important role during hematopoiesis. Previously, we had shown that the growth of a v-Src-transformed myeloid cell line was markedly more inhibited by TGF-beta treatment when compared with the wild-type myeloid cell line. To investigate the increased growth sensitivity of the v-Src-transformed myeloid cell line, 32D-src, to TGF-beta, we examined expression of the TGF-beta type II receptor (TGF-beta RII) gene in myeloid cell lines. Northem blot analysis showed that expression of approximately 8- and 6-kb species of TGF-beta RII transcripts was markedly increased in the 32D-src cell line. The expression of the TGF-beta RII promoter linked to a reporter gene was increased 23-fold by v-Src. DNA transfection and electrophoretic mobility shift assay revealed that v-Src induces TGF-beta RII promoter activity through an AP1/ATF2-like sequence (-219 to -172), ETS binding sites (+1 to +36), and the inverted CCAAT box (-81 to -77). Novel DNA-protein complexes with ETS binding sites are significantly increased in v-src-transformed cell lines compared with the control cell line. These results suggest that v-Src induces activity of the TGF-beta RII promoter through multiple elements by inducing expression of nuclear proteins interacting with these elements. SN - 1044-9523 UR - https://www.unboundmedicine.com/medline/citation/11205746/Mechanism_of_induction_of_transforming_growth_factor_beta_type_II_receptor_gene_expression_by_v_Src_in_murine_myeloid_cells_ L2 - http://cgd.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11205746 DB - PRIME DP - Unbound Medicine ER -