Tags

Type your tag names separated by a space and hit enter

Requirement of mitogen-activated protein kinases and I kappa B phosphorylation for induction of proinflammatory cytokines synthesis by macrophages indicates functional similarity of receptors triggered by glycosylphosphatidylinositol anchors from parasitic protozoa and bacterial lipopolysaccharide.
J Immunol. 2001 Mar 01; 166(5):3423-31.JI

Abstract

In the present study, we evaluated the ability of GPI-anchored mucin-like glycoproteins purified from Trypanosoma cruzi trypomastigotes (tGPI-mucin) to trigger phosphorylation of different mitogen-activated protein kinases (MAPKs) and related transcription factors in inflammatory macrophages. Kinetic experiments show that the peak of extracellular signal-related kinase (ERK)-1/ERK-2, stress-activated protein kinase (SAPK) kinase-1/mitogen-activated protein kinase (MAPK) kinase-4, and p38/SAPK-2, phosphorylation occurs between 15 and 30 min after macrophage stimulation with tGPI-mucin or GPI anchors highly purified from tGPI-mucins (tGPI). The use of the specific inhibitors of ERK-1/ERK-2 (PD 98059) and p38/SAPK-2 (SB 203580) phosphorylation also indicates the role of MAPKs, with possible involvement of cAMP response element binding protein, in triggering TNF-alpha and IL-12 synthesis by IFN-gamma-primed-macrophages exposed to tGPI or tGPI-mucin. In addition, tGPI-mucin and tGPI were able to induce phosphorylation of I kappa B, and the use of SN50 peptide, an inhibitor of NF-kappa B translocation, resulted in 70% of TNF-alpha synthesis by macrophages exposed to tGPI-mucin. Finally, the similarity of patterns of MAPK and I kappa B phosphorylation, the concentration of drugs required to inhibit cytokine synthesis, as well as cross-tolerization exhibited by macrophages exposed to tGPI, tGPI-mucin, or bacterial LPS, suggest that receptors with the same functional properties are triggered by these different microbial glycoconjugates.

Authors+Show Affiliations

René Rachou Research Center-Fundaçao Oswaldo Cruz, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11207300

Citation

Ropert, C, et al. "Requirement of Mitogen-activated Protein Kinases and I Kappa B Phosphorylation for Induction of Proinflammatory Cytokines Synthesis By Macrophages Indicates Functional Similarity of Receptors Triggered By Glycosylphosphatidylinositol Anchors From Parasitic Protozoa and Bacterial Lipopolysaccharide." Journal of Immunology (Baltimore, Md. : 1950), vol. 166, no. 5, 2001, pp. 3423-31.
Ropert C, Almeida IC, Closel M, et al. Requirement of mitogen-activated protein kinases and I kappa B phosphorylation for induction of proinflammatory cytokines synthesis by macrophages indicates functional similarity of receptors triggered by glycosylphosphatidylinositol anchors from parasitic protozoa and bacterial lipopolysaccharide. J Immunol. 2001;166(5):3423-31.
Ropert, C., Almeida, I. C., Closel, M., Travassos, L. R., Ferguson, M. A., Cohen, P., & Gazzinelli, R. T. (2001). Requirement of mitogen-activated protein kinases and I kappa B phosphorylation for induction of proinflammatory cytokines synthesis by macrophages indicates functional similarity of receptors triggered by glycosylphosphatidylinositol anchors from parasitic protozoa and bacterial lipopolysaccharide. Journal of Immunology (Baltimore, Md. : 1950), 166(5), 3423-31.
Ropert C, et al. Requirement of Mitogen-activated Protein Kinases and I Kappa B Phosphorylation for Induction of Proinflammatory Cytokines Synthesis By Macrophages Indicates Functional Similarity of Receptors Triggered By Glycosylphosphatidylinositol Anchors From Parasitic Protozoa and Bacterial Lipopolysaccharide. J Immunol. 2001 Mar 1;166(5):3423-31. PubMed PMID: 11207300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Requirement of mitogen-activated protein kinases and I kappa B phosphorylation for induction of proinflammatory cytokines synthesis by macrophages indicates functional similarity of receptors triggered by glycosylphosphatidylinositol anchors from parasitic protozoa and bacterial lipopolysaccharide. AU - Ropert,C, AU - Almeida,I C, AU - Closel,M, AU - Travassos,L R, AU - Ferguson,M A, AU - Cohen,P, AU - Gazzinelli,R T, PY - 2001/2/24/pubmed PY - 2001/5/1/medline PY - 2001/2/24/entrez SP - 3423 EP - 31 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 166 IS - 5 N2 - In the present study, we evaluated the ability of GPI-anchored mucin-like glycoproteins purified from Trypanosoma cruzi trypomastigotes (tGPI-mucin) to trigger phosphorylation of different mitogen-activated protein kinases (MAPKs) and related transcription factors in inflammatory macrophages. Kinetic experiments show that the peak of extracellular signal-related kinase (ERK)-1/ERK-2, stress-activated protein kinase (SAPK) kinase-1/mitogen-activated protein kinase (MAPK) kinase-4, and p38/SAPK-2, phosphorylation occurs between 15 and 30 min after macrophage stimulation with tGPI-mucin or GPI anchors highly purified from tGPI-mucins (tGPI). The use of the specific inhibitors of ERK-1/ERK-2 (PD 98059) and p38/SAPK-2 (SB 203580) phosphorylation also indicates the role of MAPKs, with possible involvement of cAMP response element binding protein, in triggering TNF-alpha and IL-12 synthesis by IFN-gamma-primed-macrophages exposed to tGPI or tGPI-mucin. In addition, tGPI-mucin and tGPI were able to induce phosphorylation of I kappa B, and the use of SN50 peptide, an inhibitor of NF-kappa B translocation, resulted in 70% of TNF-alpha synthesis by macrophages exposed to tGPI-mucin. Finally, the similarity of patterns of MAPK and I kappa B phosphorylation, the concentration of drugs required to inhibit cytokine synthesis, as well as cross-tolerization exhibited by macrophages exposed to tGPI, tGPI-mucin, or bacterial LPS, suggest that receptors with the same functional properties are triggered by these different microbial glycoconjugates. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/11207300/Requirement_of_mitogen_activated_protein_kinases_and_I_kappa_B_phosphorylation_for_induction_of_proinflammatory_cytokines_synthesis_by_macrophages_indicates_functional_similarity_of_receptors_triggered_by_glycosylphosphatidylinositol_anchors_from_parasitic_protozoa_and_bacterial_lipopolysaccharide_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=11207300 DB - PRIME DP - Unbound Medicine ER -