Tags

Type your tag names separated by a space and hit enter

[Purification and biochemical characterization of high-molecular-weight-glutenin subunits 14 and 15].
Yi Chuan Xue Bao. 2001; 28(1):46-51.YC

Abstract

The high molecular weight glutenin subunits 14 and 15 were purified from cultivars of bread wheat (Triticum aestivum) Xiaoyan 6 by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) appled a new method for visualizing protein in gels. N-terminal amino acid sequences were homologous comparing with other High-Molecular-Weight glutenin subunits. The result suggested that they were basic protein analyzed by two-dimensional electrophoresis of IEF(Isoelectric Focussing) x SDS-PAGE and NEPHGE(Non-Equilibrium PI-gradient Electrophoresis) x SDS-PAGE.

Authors+Show Affiliations

Deng ZY
Northwestern Agricultural-Forestry University of Science & Technical, Yangling Shaanxi 712100, China.
Zhao HX
No affiliation info available
Fan SH
No affiliation info available
Ji WQ
No affiliation info available
Guo AG
No affiliation info available
Xue XZ
No affiliation info available

MeSH

Amino Acid SequenceElectrophoresis, Gel, Two-DimensionalGlutensMolecular Sequence DataMolecular WeightProtein Subunits

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

11209711

Citation

Deng, Z Y., et al. "[Purification and Biochemical Characterization of High-molecular-weight-glutenin Subunits 14 and 15]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 28, no. 1, 2001, pp. 46-51.
Deng ZY, Zhao HX, Fan SH, et al. [Purification and biochemical characterization of high-molecular-weight-glutenin subunits 14 and 15]. Yi Chuan Xue Bao. 2001;28(1):46-51.
Deng, Z. Y., Zhao, H. X., Fan, S. H., Ji, W. Q., Guo, A. G., & Xue, X. Z. (2001). [Purification and biochemical characterization of high-molecular-weight-glutenin subunits 14 and 15]. Yi Chuan Xue Bao = Acta Genetica Sinica, 28(1), 46-51.
Deng ZY, et al. [Purification and Biochemical Characterization of High-molecular-weight-glutenin Subunits 14 and 15]. Yi Chuan Xue Bao. 2001;28(1):46-51. PubMed PMID: 11209711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Purification and biochemical characterization of high-molecular-weight-glutenin subunits 14 and 15]. AU - Deng,Z Y, AU - Zhao,H X, AU - Fan,S H, AU - Ji,W Q, AU - Guo,A G, AU - Xue,X Z, PY - 2001/2/24/pubmed PY - 2001/3/17/medline PY - 2001/2/24/entrez SP - 46 EP - 51 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 28 IS - 1 N2 - The high molecular weight glutenin subunits 14 and 15 were purified from cultivars of bread wheat (Triticum aestivum) Xiaoyan 6 by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) appled a new method for visualizing protein in gels. N-terminal amino acid sequences were homologous comparing with other High-Molecular-Weight glutenin subunits. The result suggested that they were basic protein analyzed by two-dimensional electrophoresis of IEF(Isoelectric Focussing) x SDS-PAGE and NEPHGE(Non-Equilibrium PI-gradient Electrophoresis) x SDS-PAGE. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/11209711/[Purification_and_biochemical_characterization_of_high_molecular_weight_glutenin_subunits_14_and_15]_ DB - PRIME DP - Unbound Medicine ER -