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Cancer induction in the DMBA hamster cheek pouch: a modified technique using a promoter.
Laryngoscope. 2001 Feb; 111(2):204-6.L

Abstract

OBJECTIVE

To evaluate a modified method of carcinogenesis induction using the 9,10-dimethyl-1,2-benzanthracene (DMBA) sustained-release suture technique followed by arecaidine promotion in the hamster cheek pouch model.

STUDY DESIGN

Prospective, controlled animal study.

METHODS

Number 3-0 cotton sutures were impregnated with DMBA and coated with silicone elastomer. These sutures were placed in the cheek pouch of Syrian hamsters in the submucosal space to a length of approximately 1.5 cm. The suture placement was confirmed every 2 weeks and replaced if lost. After 12 weeks, the DMBA-coated sutures were removed. The cheek pouches were everted and painted with a solution of arecaidine three times weekly for up to an additional 4 weeks or until the tumor reached a size of 100 mm2.

RESULTS

We placed sutures in 165 Syrian hamster cheek pouches. Of these, 133 hamsters (80.6%) produced squamous cell carcinomas that reached a size of 100 mm2 and then were randomly selected for treatment in a new drug trial. Twenty-six hamsters (15.8%) were found dead and 6 (3.6%) were killed because of severe inflammation.

CONCLUSIONS

The DMBA hamster cheek pouch model is a reliable and efficient animal model for inducing squamous cell carcinoma and can be used to study upper aerodigestive tract tumors.

Authors+Show Affiliations

Department of Otolaryngology, University of Tennessee School of Medicine, Memphis 38163, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11210861

Citation

Wani, M K., et al. "Cancer Induction in the DMBA Hamster Cheek Pouch: a Modified Technique Using a Promoter." The Laryngoscope, vol. 111, no. 2, 2001, pp. 204-6.
Wani MK, Yarber RH, Ahmed A, et al. Cancer induction in the DMBA hamster cheek pouch: a modified technique using a promoter. Laryngoscope. 2001;111(2):204-6.
Wani, M. K., Yarber, R. H., Ahmed, A., Hengesteg, A., & Robbins, K. T. (2001). Cancer induction in the DMBA hamster cheek pouch: a modified technique using a promoter. The Laryngoscope, 111(2), 204-6.
Wani MK, et al. Cancer Induction in the DMBA Hamster Cheek Pouch: a Modified Technique Using a Promoter. Laryngoscope. 2001;111(2):204-6. PubMed PMID: 11210861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cancer induction in the DMBA hamster cheek pouch: a modified technique using a promoter. AU - Wani,M K, AU - Yarber,R H, AU - Ahmed,A, AU - Hengesteg,A, AU - Robbins,K T, PY - 2001/2/24/pubmed PY - 2001/3/3/medline PY - 2001/2/24/entrez SP - 204 EP - 6 JF - The Laryngoscope JO - Laryngoscope VL - 111 IS - 2 N2 - OBJECTIVE: To evaluate a modified method of carcinogenesis induction using the 9,10-dimethyl-1,2-benzanthracene (DMBA) sustained-release suture technique followed by arecaidine promotion in the hamster cheek pouch model. STUDY DESIGN: Prospective, controlled animal study. METHODS: Number 3-0 cotton sutures were impregnated with DMBA and coated with silicone elastomer. These sutures were placed in the cheek pouch of Syrian hamsters in the submucosal space to a length of approximately 1.5 cm. The suture placement was confirmed every 2 weeks and replaced if lost. After 12 weeks, the DMBA-coated sutures were removed. The cheek pouches were everted and painted with a solution of arecaidine three times weekly for up to an additional 4 weeks or until the tumor reached a size of 100 mm2. RESULTS: We placed sutures in 165 Syrian hamster cheek pouches. Of these, 133 hamsters (80.6%) produced squamous cell carcinomas that reached a size of 100 mm2 and then were randomly selected for treatment in a new drug trial. Twenty-six hamsters (15.8%) were found dead and 6 (3.6%) were killed because of severe inflammation. CONCLUSIONS: The DMBA hamster cheek pouch model is a reliable and efficient animal model for inducing squamous cell carcinoma and can be used to study upper aerodigestive tract tumors. SN - 0023-852X UR - https://www.unboundmedicine.com/medline/citation/11210861/Cancer_induction_in_the_DMBA_hamster_cheek_pouch:_a_modified_technique_using_a_promoter_ L2 - https://doi.org/10.1097/00005537-200102000-00003 DB - PRIME DP - Unbound Medicine ER -