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Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide.
Cancer Res. 2001 Jan 15; 61(2):725-31.CR

Abstract

Epidemiological studies link increased garlic (Allium sativum) consumption with a reduced incidence of colon cancer in various human populations. Experimental carcinogenesis studies in animal models and in cell culture systems indicate that several allium-derived compounds exhibit inhibitory effects and that the underlying mechanisms may involve both the initiation and promotion phases of carcinogenesis. To provide a better understanding of the effects of allium derivatives on the prevention of colon cancer, we examined two water-soluble derivatives of garlic, S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC), for their effects on proliferation and cell cycle progression in two human colon cancer cell lines, SW-480 and HT-29. For comparison, we included the compound sulindac sulfide (SS), because sulindac compounds are well-established colon cancer chemopreventive agents. We found that SAMC, but not SAC, inhibited the growth of both cell lines at doses similar to that of SS. SAMC also induced apoptosis, and this was associated with an increase in caspase3-like activity. These affects of SAMC were accompanied by induction of jun kinase activity and a marked increase in endogenous levels of reduced glutathione. Although SS caused inhibition of cell cycle progression from G1 to S, SAMC inhibited progression at G2-M, and a fraction of the SW-480 and HT-29 cells were specifically arrested in mitosis. Coadministration of SS with SAMC enhanced the growth inhibitory and apoptotic effects of SS. These findings suggest that SAMC may be useful in colon cancer prevention when used alone or in combination with SS or other chemopreventive agents.

Authors+Show Affiliations

Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11212275

Citation

Shirin, H, et al. "Antiproliferative Effects of S-allylmercaptocysteine On Colon Cancer Cells when Tested Alone or in Combination With Sulindac Sulfide." Cancer Research, vol. 61, no. 2, 2001, pp. 725-31.
Shirin H, Pinto JT, Kawabata Y, et al. Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide. Cancer Res. 2001;61(2):725-31.
Shirin, H., Pinto, J. T., Kawabata, Y., Soh, J. W., Delohery, T., Moss, S. F., Murty, V., Rivlin, R. S., Holt, P. R., & Weinstein, I. B. (2001). Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide. Cancer Research, 61(2), 725-31.
Shirin H, et al. Antiproliferative Effects of S-allylmercaptocysteine On Colon Cancer Cells when Tested Alone or in Combination With Sulindac Sulfide. Cancer Res. 2001 Jan 15;61(2):725-31. PubMed PMID: 11212275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide. AU - Shirin,H, AU - Pinto,J T, AU - Kawabata,Y, AU - Soh,J W, AU - Delohery,T, AU - Moss,S F, AU - Murty,V, AU - Rivlin,R S, AU - Holt,P R, AU - Weinstein,I B, PY - 2001/2/24/pubmed PY - 2001/3/3/medline PY - 2001/2/24/entrez SP - 725 EP - 31 JF - Cancer research JO - Cancer Res VL - 61 IS - 2 N2 - Epidemiological studies link increased garlic (Allium sativum) consumption with a reduced incidence of colon cancer in various human populations. Experimental carcinogenesis studies in animal models and in cell culture systems indicate that several allium-derived compounds exhibit inhibitory effects and that the underlying mechanisms may involve both the initiation and promotion phases of carcinogenesis. To provide a better understanding of the effects of allium derivatives on the prevention of colon cancer, we examined two water-soluble derivatives of garlic, S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC), for their effects on proliferation and cell cycle progression in two human colon cancer cell lines, SW-480 and HT-29. For comparison, we included the compound sulindac sulfide (SS), because sulindac compounds are well-established colon cancer chemopreventive agents. We found that SAMC, but not SAC, inhibited the growth of both cell lines at doses similar to that of SS. SAMC also induced apoptosis, and this was associated with an increase in caspase3-like activity. These affects of SAMC were accompanied by induction of jun kinase activity and a marked increase in endogenous levels of reduced glutathione. Although SS caused inhibition of cell cycle progression from G1 to S, SAMC inhibited progression at G2-M, and a fraction of the SW-480 and HT-29 cells were specifically arrested in mitosis. Coadministration of SS with SAMC enhanced the growth inhibitory and apoptotic effects of SS. These findings suggest that SAMC may be useful in colon cancer prevention when used alone or in combination with SS or other chemopreventive agents. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/11212275/Antiproliferative_effects_of_S_allylmercaptocysteine_on_colon_cancer_cells_when_tested_alone_or_in_combination_with_sulindac_sulfide_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11212275 DB - PRIME DP - Unbound Medicine ER -