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Genotyping of human platelet antigens in ethnic Northeastern Thais by the polymerase chain reaction-sequence specific primer technique.
J Med Assoc Thai. 2000 Nov; 83(11):1333-9.JM

Abstract

Human platelet antigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAITP), post-transfusion purpura (PTP), refractoriness to platelet transfusion therapy and population genetics. The distribution of HPA in a Northeast Thai population was studied. 300 healthy, unrelated, and ethnic Northeastern Thais were randomly selected. Using the polymerase chain reaction-sequence specific primer technique (PCR-SSP), the frequency of HPA-1, -2, -3, -4, -5 and -6 were determined. The phenotype frequencies were 100 per cent for HPA-1a, 4a, 5a, and 6a. For HPA-1b, 2a, 2b, 3a, 3b, 5b and 6b, the frequencies were 5.7, 99.7, 12.3, 78.0, 71.3, 7.3 and 3.0 per cent, respectively. The HPA-4b was not found. The HPA frequencies in our subjects were quite similar to other Asian populations but were different from Caucasians. The distribution of HPA genotypes encountered in our study indicate that HPA-1a, -4a, -4b, -5a and -6a will not be involved in NAITP, PTP and refractoriness to platelet transfusion therapy in Northeastern Thais. Moreover, HPA-1b, -2a, -2b, -3a, -3b, -5b and -6b may induce alloantibodies in these patients.

Authors+Show Affiliations

Blood Transfusion Centre, Faculty of Medicine, Khon Kaen University, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11215863

Citation

Romphruk, A V., et al. "Genotyping of Human Platelet Antigens in Ethnic Northeastern Thais By the Polymerase Chain Reaction-sequence Specific Primer Technique." Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, vol. 83, no. 11, 2000, pp. 1333-9.
Romphruk AV, Akahat J, Srivanichrak P, et al. Genotyping of human platelet antigens in ethnic Northeastern Thais by the polymerase chain reaction-sequence specific primer technique. J Med Assoc Thai. 2000;83(11):1333-9.
Romphruk, A. V., Akahat, J., Srivanichrak, P., Puapairoj, C., Romphruk, A., & Leelayuwat, C. (2000). Genotyping of human platelet antigens in ethnic Northeastern Thais by the polymerase chain reaction-sequence specific primer technique. Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 83(11), 1333-9.
Romphruk AV, et al. Genotyping of Human Platelet Antigens in Ethnic Northeastern Thais By the Polymerase Chain Reaction-sequence Specific Primer Technique. J Med Assoc Thai. 2000;83(11):1333-9. PubMed PMID: 11215863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping of human platelet antigens in ethnic Northeastern Thais by the polymerase chain reaction-sequence specific primer technique. AU - Romphruk,A V, AU - Akahat,J, AU - Srivanichrak,P, AU - Puapairoj,C, AU - Romphruk,A, AU - Leelayuwat,C, PY - 2001/2/24/pubmed PY - 2001/5/5/medline PY - 2001/2/24/entrez SP - 1333 EP - 9 JF - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JO - J Med Assoc Thai VL - 83 IS - 11 N2 - Human platelet antigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAITP), post-transfusion purpura (PTP), refractoriness to platelet transfusion therapy and population genetics. The distribution of HPA in a Northeast Thai population was studied. 300 healthy, unrelated, and ethnic Northeastern Thais were randomly selected. Using the polymerase chain reaction-sequence specific primer technique (PCR-SSP), the frequency of HPA-1, -2, -3, -4, -5 and -6 were determined. The phenotype frequencies were 100 per cent for HPA-1a, 4a, 5a, and 6a. For HPA-1b, 2a, 2b, 3a, 3b, 5b and 6b, the frequencies were 5.7, 99.7, 12.3, 78.0, 71.3, 7.3 and 3.0 per cent, respectively. The HPA-4b was not found. The HPA frequencies in our subjects were quite similar to other Asian populations but were different from Caucasians. The distribution of HPA genotypes encountered in our study indicate that HPA-1a, -4a, -4b, -5a and -6a will not be involved in NAITP, PTP and refractoriness to platelet transfusion therapy in Northeastern Thais. Moreover, HPA-1b, -2a, -2b, -3a, -3b, -5b and -6b may induce alloantibodies in these patients. SN - 0125-2208 UR - https://www.unboundmedicine.com/medline/citation/11215863/Genotyping_of_human_platelet_antigens_in_ethnic_Northeastern_Thais_by_the_polymerase_chain_reaction_sequence_specific_primer_technique_ DB - PRIME DP - Unbound Medicine ER -