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Matrix metalloproteinases and tissue inhibitors of metalloproteinases in cerebrospinal fluid differ in multiple sclerosis and Devic's neuromyelitis optica.
Brain. 2001 Mar; 124(Pt 3):493-8.B

Abstract

Matrix metalloproteinases (MMPs) are increased in the CSF of patients with multiple sclerosis. Devic's neuromyelitis optica (DNO) is a demyelinating syndrome that involves the optic nerve and cervical cord but differs pathologically from multiple sclerosis. Therefore, we hypothesized that the type of inflammatory reaction that causes MMPs to be elevated in multiple sclerosis would be absent in patients with DNO. CSF was collected from 23 patients with relapsing-remitting or secondary progressive multiple sclerosis, all of whom were experiencing acute symptoms, from seven patients with DNO, and from seven normal volunteers. Diagnoses were made according to current criteria on the basis of clinical manifestations, imaging results and CSF studies. IgG synthesis was increased in the CSF of multiple sclerosis patients but not in that of DNO patients. Zymography, reverse zymography and ELISA (enzyme-linked immunosorbent assay) were used to measure gelatinase A (MMP-2), gelatinase B (MMP-9) and tissue inhibitors of metalloproteinases (TIMPs). Zymograms showed that multiple sclerosis patients had elevated MMP-9 compared with DNO patients and controls (P: < 0.05). TIMP-1 and TIMP-2 levels were similar in all three groups. We conclude that multiple sclerosis patients have higher MMP-9 levels in the CSF than patients with DNO, which supports the different pathological mechanisms of these diseases.

Authors+Show Affiliations

Department of Neurology, University of New Mexico Health Sciences Center, Albuquerque, NM 87107, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11222449

Citation

Mandler, R N., et al. "Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Cerebrospinal Fluid Differ in Multiple Sclerosis and Devic's Neuromyelitis Optica." Brain : a Journal of Neurology, vol. 124, no. Pt 3, 2001, pp. 493-8.
Mandler RN, Dencoff JD, Midani F, et al. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in cerebrospinal fluid differ in multiple sclerosis and Devic's neuromyelitis optica. Brain. 2001;124(Pt 3):493-8.
Mandler, R. N., Dencoff, J. D., Midani, F., Ford, C. C., Ahmed, W., & Rosenberg, G. A. (2001). Matrix metalloproteinases and tissue inhibitors of metalloproteinases in cerebrospinal fluid differ in multiple sclerosis and Devic's neuromyelitis optica. Brain : a Journal of Neurology, 124(Pt 3), 493-8.
Mandler RN, et al. Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Cerebrospinal Fluid Differ in Multiple Sclerosis and Devic's Neuromyelitis Optica. Brain. 2001;124(Pt 3):493-8. PubMed PMID: 11222449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix metalloproteinases and tissue inhibitors of metalloproteinases in cerebrospinal fluid differ in multiple sclerosis and Devic's neuromyelitis optica. AU - Mandler,R N, AU - Dencoff,J D, AU - Midani,F, AU - Ford,C C, AU - Ahmed,W, AU - Rosenberg,G A, PY - 2001/2/27/pubmed PY - 2001/4/6/medline PY - 2001/2/27/entrez SP - 493 EP - 8 JF - Brain : a journal of neurology JO - Brain VL - 124 IS - Pt 3 N2 - Matrix metalloproteinases (MMPs) are increased in the CSF of patients with multiple sclerosis. Devic's neuromyelitis optica (DNO) is a demyelinating syndrome that involves the optic nerve and cervical cord but differs pathologically from multiple sclerosis. Therefore, we hypothesized that the type of inflammatory reaction that causes MMPs to be elevated in multiple sclerosis would be absent in patients with DNO. CSF was collected from 23 patients with relapsing-remitting or secondary progressive multiple sclerosis, all of whom were experiencing acute symptoms, from seven patients with DNO, and from seven normal volunteers. Diagnoses were made according to current criteria on the basis of clinical manifestations, imaging results and CSF studies. IgG synthesis was increased in the CSF of multiple sclerosis patients but not in that of DNO patients. Zymography, reverse zymography and ELISA (enzyme-linked immunosorbent assay) were used to measure gelatinase A (MMP-2), gelatinase B (MMP-9) and tissue inhibitors of metalloproteinases (TIMPs). Zymograms showed that multiple sclerosis patients had elevated MMP-9 compared with DNO patients and controls (P: < 0.05). TIMP-1 and TIMP-2 levels were similar in all three groups. We conclude that multiple sclerosis patients have higher MMP-9 levels in the CSF than patients with DNO, which supports the different pathological mechanisms of these diseases. SN - 0006-8950 UR - https://www.unboundmedicine.com/medline/citation/11222449/Matrix_metalloproteinases_and_tissue_inhibitors_of_metalloproteinases_in_cerebrospinal_fluid_differ_in_multiple_sclerosis_and_Devic's_neuromyelitis_optica_ L2 - https://academic.oup.com/brain/article-lookup/doi/10.1093/brain/124.3.493 DB - PRIME DP - Unbound Medicine ER -