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Protein-DNA binding and CpG methylation at nucleotide resolution of latency-associated promoters Qp, Cp, and LMP1p of Epstein-Barr virus.
J Virol. 2001 Mar; 75(6):2584-96.JV

Abstract

Epstein-Barr viral (EBV) latency-associated promoters Qp, Cp, and LMP1p are crucial for the regulated expression of the EBNA and LMP transcripts in dependence of the latency type. By transient transfection and in vitro binding analyses, many promoter elements and transcription factors have previously been shown to be involved in the activities of these promoters. However, the latency promoters have only partially been examined at the nucleotide level in vivo. Therefore, we undertook a comprehensive analysis of in vivo protein binding and CpG methylation patterns at these promoters in five representative cell lines and correlated the results with the known in vitro binding data and activities of these promoters from previous transfection experiments. Promoter activity inversely correlated with the methylation state of promoters, although Qp was a remarkable exception. Novel protein binding data were obtained for all promoters. For Cp, binding correlated well with promoter activity; for LMP1p and Qp, binding patterns looked similar regardless of promoter activity.

Authors+Show Affiliations

Microbiological Research Group, National Center for Epidemiology, H-1529 Budapest, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11222681

Citation

Salamon, D, et al. "Protein-DNA Binding and CpG Methylation at Nucleotide Resolution of Latency-associated Promoters Qp, Cp, and LMP1p of Epstein-Barr Virus." Journal of Virology, vol. 75, no. 6, 2001, pp. 2584-96.
Salamon D, Takacs M, Ujvari D, et al. Protein-DNA binding and CpG methylation at nucleotide resolution of latency-associated promoters Qp, Cp, and LMP1p of Epstein-Barr virus. J Virol. 2001;75(6):2584-96.
Salamon, D., Takacs, M., Ujvari, D., Uhlig, J., Wolf, H., Minarovits, J., & Niller, H. H. (2001). Protein-DNA binding and CpG methylation at nucleotide resolution of latency-associated promoters Qp, Cp, and LMP1p of Epstein-Barr virus. Journal of Virology, 75(6), 2584-96.
Salamon D, et al. Protein-DNA Binding and CpG Methylation at Nucleotide Resolution of Latency-associated Promoters Qp, Cp, and LMP1p of Epstein-Barr Virus. J Virol. 2001;75(6):2584-96. PubMed PMID: 11222681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein-DNA binding and CpG methylation at nucleotide resolution of latency-associated promoters Qp, Cp, and LMP1p of Epstein-Barr virus. AU - Salamon,D, AU - Takacs,M, AU - Ujvari,D, AU - Uhlig,J, AU - Wolf,H, AU - Minarovits,J, AU - Niller,H H, PY - 2001/2/27/pubmed PY - 2001/4/6/medline PY - 2001/2/27/entrez SP - 2584 EP - 96 JF - Journal of virology JO - J. Virol. VL - 75 IS - 6 N2 - Epstein-Barr viral (EBV) latency-associated promoters Qp, Cp, and LMP1p are crucial for the regulated expression of the EBNA and LMP transcripts in dependence of the latency type. By transient transfection and in vitro binding analyses, many promoter elements and transcription factors have previously been shown to be involved in the activities of these promoters. However, the latency promoters have only partially been examined at the nucleotide level in vivo. Therefore, we undertook a comprehensive analysis of in vivo protein binding and CpG methylation patterns at these promoters in five representative cell lines and correlated the results with the known in vitro binding data and activities of these promoters from previous transfection experiments. Promoter activity inversely correlated with the methylation state of promoters, although Qp was a remarkable exception. Novel protein binding data were obtained for all promoters. For Cp, binding correlated well with promoter activity; for LMP1p and Qp, binding patterns looked similar regardless of promoter activity. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/11222681/Protein_DNA_binding_and_CpG_methylation_at_nucleotide_resolution_of_latency_associated_promoters_Qp_Cp_and_LMP1p_of_Epstein_Barr_virus_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=11222681 DB - PRIME DP - Unbound Medicine ER -