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Effects of intraarticular glucocorticoids on macrophage infiltration and mediators of joint damage in osteoarthritis synovial membranes: findings in a double-blind, placebo-controlled study.
Arthritis Rheum. 2001 Feb; 44(2):343-50.AR

Abstract

OBJECTIVE

To investigate the effects of intraarticular glucocorticoid treatment on macrophage infiltration, the expression of the chemokines monocyte chemoattractant protein 1 (MCP-1) and macrophage inflammatory protein 1alpha (MIP-1alpha), and the expression of matrix metalloproteinases 1 and 3 (MMPs 1 and 3) and their inhibitors, the tissue inhibitors of metalloproteinases 1 and 2 (TIMPs 1 and 2), in osteoarthritis (OA) synovial membranes.

METHODS

Forty patients underwent arthroscopic biopsy before and 1 month after intraarticular injection of glucocorticoids. Twenty-one patients received 120 mg of methylprednisolone acetate (Depo-Medrol; Upjohn, Kalamazoo, MI), and 20 patients received placebo (1 patient received placebo in 1 knee and methylprednisolone acetate in the other). Immunoperoxidase staining for the expression of CD68, MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 was performed, and the immunostaining was quantified by color video image analysis.

RESULTS

CD68, MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining was observed in all synovial membranes. Intraarticular glucocorticoid treatment was associated with a small (30%) but statistically significant (P = 0.048) reduction in CD68+ macrophage staining in the synovial lining layer, but there was no change in the CD68 expression in the synovial sublining layer. No significant differences were observed for MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining in the synovial lining or sublining layers.

CONCLUSION

Intraarticular glucocorticoids may reduce CD68+ macrophage infiltration into the synovial lining layer, but not the expression of MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 in the synovial membrane, in patients with OA.

Authors+Show Affiliations

Prince of Wales Hospital, Sydney, New South Wales, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11229465

Citation

Young, L, et al. "Effects of Intraarticular Glucocorticoids On Macrophage Infiltration and Mediators of Joint Damage in Osteoarthritis Synovial Membranes: Findings in a Double-blind, Placebo-controlled Study." Arthritis and Rheumatism, vol. 44, no. 2, 2001, pp. 343-50.
Young L, Katrib A, Cuello C, et al. Effects of intraarticular glucocorticoids on macrophage infiltration and mediators of joint damage in osteoarthritis synovial membranes: findings in a double-blind, placebo-controlled study. Arthritis Rheum. 2001;44(2):343-50.
Young, L., Katrib, A., Cuello, C., Vollmer-Conna, U., Bertouch, J. V., Roberts-Thomson, P. J., Ahern, M. J., Smith, M. D., & Youssef, P. P. (2001). Effects of intraarticular glucocorticoids on macrophage infiltration and mediators of joint damage in osteoarthritis synovial membranes: findings in a double-blind, placebo-controlled study. Arthritis and Rheumatism, 44(2), 343-50.
Young L, et al. Effects of Intraarticular Glucocorticoids On Macrophage Infiltration and Mediators of Joint Damage in Osteoarthritis Synovial Membranes: Findings in a Double-blind, Placebo-controlled Study. Arthritis Rheum. 2001;44(2):343-50. PubMed PMID: 11229465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of intraarticular glucocorticoids on macrophage infiltration and mediators of joint damage in osteoarthritis synovial membranes: findings in a double-blind, placebo-controlled study. AU - Young,L, AU - Katrib,A, AU - Cuello,C, AU - Vollmer-Conna,U, AU - Bertouch,J V, AU - Roberts-Thomson,P J, AU - Ahern,M J, AU - Smith,M D, AU - Youssef,P P, PY - 2001/3/7/pubmed PY - 2001/3/27/medline PY - 2001/3/7/entrez SP - 343 EP - 50 JF - Arthritis and rheumatism JO - Arthritis Rheum VL - 44 IS - 2 N2 - OBJECTIVE: To investigate the effects of intraarticular glucocorticoid treatment on macrophage infiltration, the expression of the chemokines monocyte chemoattractant protein 1 (MCP-1) and macrophage inflammatory protein 1alpha (MIP-1alpha), and the expression of matrix metalloproteinases 1 and 3 (MMPs 1 and 3) and their inhibitors, the tissue inhibitors of metalloproteinases 1 and 2 (TIMPs 1 and 2), in osteoarthritis (OA) synovial membranes. METHODS: Forty patients underwent arthroscopic biopsy before and 1 month after intraarticular injection of glucocorticoids. Twenty-one patients received 120 mg of methylprednisolone acetate (Depo-Medrol; Upjohn, Kalamazoo, MI), and 20 patients received placebo (1 patient received placebo in 1 knee and methylprednisolone acetate in the other). Immunoperoxidase staining for the expression of CD68, MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 was performed, and the immunostaining was quantified by color video image analysis. RESULTS: CD68, MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining was observed in all synovial membranes. Intraarticular glucocorticoid treatment was associated with a small (30%) but statistically significant (P = 0.048) reduction in CD68+ macrophage staining in the synovial lining layer, but there was no change in the CD68 expression in the synovial sublining layer. No significant differences were observed for MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining in the synovial lining or sublining layers. CONCLUSION: Intraarticular glucocorticoids may reduce CD68+ macrophage infiltration into the synovial lining layer, but not the expression of MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 in the synovial membrane, in patients with OA. SN - 0004-3591 UR - https://www.unboundmedicine.com/medline/citation/11229465/Effects_of_intraarticular_glucocorticoids_on_macrophage_infiltration_and_mediators_of_joint_damage_in_osteoarthritis_synovial_membranes:_findings_in_a_double_blind_placebo_controlled_study_ L2 - https://doi.org/10.1002/1529-0131(200102)44:2<343::AID-ANR52>3.0.CO;2-Q DB - PRIME DP - Unbound Medicine ER -