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Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients.
J Clin Microbiol. 2001 Mar; 39(3):977-82.JC

Abstract

Using a serotype-specific monoclonal antibody (MAb) of dengue virus type 1 (DEN-1), 15F3-1, we identified the B-cell epitope of DEN-1 from a random peptide library displayed on phage. Fourteen immunopositive phage clones that bound specifically to MAb 15F3-1 were selected. These phage-borne peptides had a consensus motif of HxYaWb (a = S/T, b = K/H/R) that mimicked the sequence HKYSWK, which corresponded to amino acid residues 111 to 116 of the nonstructural protein 1 (NS1) of DEN-1. Among the four synthetic peptides corresponding to amino acid residues 110 to 117 of the NS1 of DEN-1, -2, -3, and -4, only one peptide, EHKYSWKS (P14M) of DEN-1, was found to bind to 15F3-1 specifically. Furthermore, P14M was shown to inhibit the binding of phage particles to 15F3-1 in a competitive inhibition assay. Histidine(111) (His(111)) was crucial to the binding of P14M to 15F3-1, since its binding activity dramatically reduced when it changed to leucine(111) (Leu(111)). This epitope-based peptide demonstrated its clinical diagnostic potential when it reacted with a high degree of specificity with serum samples obtained from both DEN-1-infected rabbits and patients. Based on these observations, our DEN-1 epitope-based serologic test could be useful in laboratory viral diagnosis and in understanding the pathogenesis of DEN-1.

Authors+Show Affiliations

Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, San-Hsia, Taipei, Taiwan, Republic of China. ipmc3@ms29.hinet.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11230414

Citation

Wu, H C., et al. "Identification of B-cell Epitope of Dengue Virus Type 1 and Its Application in Diagnosis of Patients." Journal of Clinical Microbiology, vol. 39, no. 3, 2001, pp. 977-82.
Wu HC, Huang YL, Chao TT, et al. Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients. J Clin Microbiol. 2001;39(3):977-82.
Wu, H. C., Huang, Y. L., Chao, T. T., Jan, J. T., Huang, J. L., Chiang, H. Y., King, C. C., & Shaio, M. F. (2001). Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients. Journal of Clinical Microbiology, 39(3), 977-82.
Wu HC, et al. Identification of B-cell Epitope of Dengue Virus Type 1 and Its Application in Diagnosis of Patients. J Clin Microbiol. 2001;39(3):977-82. PubMed PMID: 11230414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients. AU - Wu,H C, AU - Huang,Y L, AU - Chao,T T, AU - Jan,J T, AU - Huang,J L, AU - Chiang,H Y, AU - King,C C, AU - Shaio,M F, PY - 2001/3/7/pubmed PY - 2001/7/20/medline PY - 2001/3/7/entrez SP - 977 EP - 82 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 39 IS - 3 N2 - Using a serotype-specific monoclonal antibody (MAb) of dengue virus type 1 (DEN-1), 15F3-1, we identified the B-cell epitope of DEN-1 from a random peptide library displayed on phage. Fourteen immunopositive phage clones that bound specifically to MAb 15F3-1 were selected. These phage-borne peptides had a consensus motif of HxYaWb (a = S/T, b = K/H/R) that mimicked the sequence HKYSWK, which corresponded to amino acid residues 111 to 116 of the nonstructural protein 1 (NS1) of DEN-1. Among the four synthetic peptides corresponding to amino acid residues 110 to 117 of the NS1 of DEN-1, -2, -3, and -4, only one peptide, EHKYSWKS (P14M) of DEN-1, was found to bind to 15F3-1 specifically. Furthermore, P14M was shown to inhibit the binding of phage particles to 15F3-1 in a competitive inhibition assay. Histidine(111) (His(111)) was crucial to the binding of P14M to 15F3-1, since its binding activity dramatically reduced when it changed to leucine(111) (Leu(111)). This epitope-based peptide demonstrated its clinical diagnostic potential when it reacted with a high degree of specificity with serum samples obtained from both DEN-1-infected rabbits and patients. Based on these observations, our DEN-1 epitope-based serologic test could be useful in laboratory viral diagnosis and in understanding the pathogenesis of DEN-1. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11230414/Identification_of_B_cell_epitope_of_dengue_virus_type_1_and_its_application_in_diagnosis_of_patients_ DB - PRIME DP - Unbound Medicine ER -