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Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.
J Clin Microbiol 2001; 39(3):1008-16JC

Abstract

The performance of the Becton Dickinson BDProbe Tec ET System Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays (BD Biosciences, Sparks, Md.) was evaluated in a multicenter study. Specimens were collected from 2,109 men and women, with or without symptoms, attending sexually transmitted disease, family planning, and obstetrics and gynecology clinics. Both swab and urine samples were collected, and the results obtained from 4,131 specimens were compared to those from culture and the LCx nucleic acid amplification test (Abbott Industries, Abbott Park, Ill.). PCR and cytospin of the culture transport medium with chlamydia direct fluorescent antibody staining were used to adjudicate chlamydia culture-negative results. Sensitivity and specificity were calculated both with and without use of the amplification control (AC), with little apparent difference in the results. Without the AC result, sensitivity for C. trachomatis and N. gonorrhoeae were 92.8 and 96.6%, respectively, for cervical swabs and 80.5 and 84.9% for urine from women. C. trachomatis and N. gonorrhoeae sensitivities were 92.5 and 98.5%, respectively, for male urethral swabs and 93.1 and 97.9% for urine from men. This amplified DNA system for simultaneous detection of chlamydial and gonococcal infections demonstrated superior sensitivity compared to chlamydia culture and has performance characteristics comparable to those of other commercially available nucleic acid-based assays for these organisms.

Authors+Show Affiliations

Indiana University School of Medicine, Indianapolis, IN 46202, USA. bvanderp@iupui.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11230419

Citation

Van Der Pol, B, et al. "Multicenter Evaluation of the BDProbeTec ET System for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Urine Specimens, Female Endocervical Swabs, and Male Urethral Swabs." Journal of Clinical Microbiology, vol. 39, no. 3, 2001, pp. 1008-16.
Van Der Pol B, Ferrero DV, Buck-Barrington L, et al. Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J Clin Microbiol. 2001;39(3):1008-16.
Van Der Pol, B., Ferrero, D. V., Buck-Barrington, L., Hook, E., Lenderman, C., Quinn, T., ... Jones, R. B. (2001). Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. Journal of Clinical Microbiology, 39(3), pp. 1008-16.
Van Der Pol B, et al. Multicenter Evaluation of the BDProbeTec ET System for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Urine Specimens, Female Endocervical Swabs, and Male Urethral Swabs. J Clin Microbiol. 2001;39(3):1008-16. PubMed PMID: 11230419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. AU - Van Der Pol,B, AU - Ferrero,D V, AU - Buck-Barrington,L, AU - Hook,E,3rd AU - Lenderman,C, AU - Quinn,T, AU - Gaydos,C A, AU - Lovchik,J, AU - Schachter,J, AU - Moncada,J, AU - Hall,G, AU - Tuohy,M J, AU - Jones,R B, PY - 2001/3/7/pubmed PY - 2001/7/20/medline PY - 2001/3/7/entrez SP - 1008 EP - 16 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 39 IS - 3 N2 - The performance of the Becton Dickinson BDProbe Tec ET System Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays (BD Biosciences, Sparks, Md.) was evaluated in a multicenter study. Specimens were collected from 2,109 men and women, with or without symptoms, attending sexually transmitted disease, family planning, and obstetrics and gynecology clinics. Both swab and urine samples were collected, and the results obtained from 4,131 specimens were compared to those from culture and the LCx nucleic acid amplification test (Abbott Industries, Abbott Park, Ill.). PCR and cytospin of the culture transport medium with chlamydia direct fluorescent antibody staining were used to adjudicate chlamydia culture-negative results. Sensitivity and specificity were calculated both with and without use of the amplification control (AC), with little apparent difference in the results. Without the AC result, sensitivity for C. trachomatis and N. gonorrhoeae were 92.8 and 96.6%, respectively, for cervical swabs and 80.5 and 84.9% for urine from women. C. trachomatis and N. gonorrhoeae sensitivities were 92.5 and 98.5%, respectively, for male urethral swabs and 93.1 and 97.9% for urine from men. This amplified DNA system for simultaneous detection of chlamydial and gonococcal infections demonstrated superior sensitivity compared to chlamydia culture and has performance characteristics comparable to those of other commercially available nucleic acid-based assays for these organisms. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11230419/Multicenter_evaluation_of_the_BDProbeTec_ET_System_for_detection_of_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_in_urine_specimens_female_endocervical_swabs_and_male_urethral_swabs_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=11230419 DB - PRIME DP - Unbound Medicine ER -