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An iron-regulated ferric reductase associated with the absorption of dietary iron.
Science. 2001 Mar 02; 291(5509):1755-9.Sci

Abstract

The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal mucosa. Dcytb shared between 45 and 50% similarity to the cytochrome b561 family of plasma membrane reductases, was highly expressed in the brush-border membrane of duodenal enterocytes, and induced ferric reductase activity when expressed in Xenopus oocytes and cultured cells. Duodenal expression levels of Dcytb messenger RNA and protein were regulated by changes in physiological modulators of iron absorption. Thus, Dcytb provides an important element in the iron absorption pathway.

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Authors+Show Affiliations

McKie AT
Department of Molecular Medicine, Guy's, King's and St. Thomas' School of Medicine, King's College London, Rayne Institute, Denmark Hill Campus, 123 Coldharbour Lane, London SE5 9NU, UK. andrew.t.mckie@kcl.ac.uk
Barrow D
No affiliation info available
Latunde-Dada GO
No affiliation info available
Rolfs A
No affiliation info available
Sager G
No affiliation info available
Mudaly E
No affiliation info available
Mudaly M
No affiliation info available
Richardson C
No affiliation info available
Barlow D
No affiliation info available
Bomford A
No affiliation info available
Peters TJ
No affiliation info available
Raja KB
No affiliation info available
Shirali S
No affiliation info available
Hediger MA
No affiliation info available
Farzaneh F
No affiliation info available
Simpson RJ
No affiliation info available

MeSH

Amino Acid SequenceAnemiaAnimalsCell LineCloning, MolecularCytochrome b GroupDNA, ComplementaryDuodenumEnterocytesEnzyme InductionFerric CompoundsHypoxiaIntestinal AbsorptionIntestinal MucosaIron, DietaryMaleMiceMicrovilliMolecular Sequence DataNitroblue TetrazoliumOocytesOxidation-ReductionOxidoreductasesRNA, MessengerTetrazolium SaltsThiazolesTransfectionUp-RegulationXenopus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11230685

Clinical Trial Links

Evaluation of Calcium, Vitamin D, Magnesium, and Zinc Absorption in Children

Citation

McKie, A T., et al. "An Iron-regulated Ferric Reductase Associated With the Absorption of Dietary Iron." Science (New York, N.Y.), vol. 291, no. 5509, 2001, pp. 1755-9.
McKie AT, Barrow D, Latunde-Dada GO, et al. An iron-regulated ferric reductase associated with the absorption of dietary iron. Science. 2001;291(5509):1755-9.
McKie, A. T., Barrow, D., Latunde-Dada, G. O., Rolfs, A., Sager, G., Mudaly, E., Mudaly, M., Richardson, C., Barlow, D., Bomford, A., Peters, T. J., Raja, K. B., Shirali, S., Hediger, M. A., Farzaneh, F., & Simpson, R. J. (2001). An iron-regulated ferric reductase associated with the absorption of dietary iron. Science (New York, N.Y.), 291(5509), 1755-9.
McKie AT, et al. An Iron-regulated Ferric Reductase Associated With the Absorption of Dietary Iron. Science. 2001 Mar 2;291(5509):1755-9. PubMed PMID: 11230685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An iron-regulated ferric reductase associated with the absorption of dietary iron. AU - McKie,A T, AU - Barrow,D, AU - Latunde-Dada,G O, AU - Rolfs,A, AU - Sager,G, AU - Mudaly,E, AU - Mudaly,M, AU - Richardson,C, AU - Barlow,D, AU - Bomford,A, AU - Peters,T J, AU - Raja,K B, AU - Shirali,S, AU - Hediger,M A, AU - Farzaneh,F, AU - Simpson,R J, Y1 - 2001/02/01/ PY - 2001/3/7/pubmed PY - 2001/4/6/medline PY - 2001/3/7/entrez SP - 1755 EP - 9 JF - Science (New York, N.Y.) JO - Science VL - 291 IS - 5509 N2 - The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal mucosa. Dcytb shared between 45 and 50% similarity to the cytochrome b561 family of plasma membrane reductases, was highly expressed in the brush-border membrane of duodenal enterocytes, and induced ferric reductase activity when expressed in Xenopus oocytes and cultured cells. Duodenal expression levels of Dcytb messenger RNA and protein were regulated by changes in physiological modulators of iron absorption. Thus, Dcytb provides an important element in the iron absorption pathway. SN - 0036-8075 UR - https://www.unboundmedicine.com/medline/citation/11230685/An_iron_regulated_ferric_reductase_associated_with_the_absorption_of_dietary_iron_ L2 - https:///www.science.org/doi/10.1126/science.1057206?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -