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Sensitive determination of n-hexane and cyclohexane in human body fluids by capillary gas chromatography with cryogenic oven trapping.
J AOAC Int. 2001 Jan-Feb; 84(1):19-23.JA

Abstract

A sensitive method was developed for determination of n-hexane and cyclohexane in human body fluids by headspace capillary gas chromatography (GC) with cryogenic oven trapping. Whole blood and urine samples containing n-hexane and cyclohexane were heated in a 7.5 mL vial at 70 degrees C for 15 min, and 5 mL of the headspace vapor was drawn into a glass syringe. All vapor was introduced through an injection port of a GC instrument in the splitless mode into an Rtx-Volatiles middle-bore capillary column at an oven temperature of -40 degrees C for trapping volatile compounds. The oven temperature was programmed to 180 degrees C for GC with flame ionization detection. These conditions gave sharp peaks for both n-hexane and cyclohexane, a good separation of each peak, and low background impurities for whole blood and urine. The extraction efficiencies of n-hexane and cyclohexane were 13.2-30.3% for whole blood and 12.7-20.7% for urine. The coefficients of within-day variation in terms of extraction efficiency of both compounds were 5.0-9.5% for whole blood and 3.8-10.8% for urine; those of day-to-day variation for the compounds were not greater than 16.6%. The regression equations for n-hexane and cyclohexane showed good linearity in the range of 5-500 ng/0.5 mL for whole blood and urine. The detection limits (signal-to-noise ratio = 3) for both compounds were 1.2 and 0.5 ng/0.5 mL for whole blood and urine, respectively. The data on n-hexane or cyclohexane in rat blood after inhalation of each compound are also presented.

Authors+Show Affiliations

Showa University School of Medicine, Department of Legal Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11234809

Citation

Kondo, K, et al. "Sensitive Determination of N-hexane and Cyclohexane in Human Body Fluids By Capillary Gas Chromatography With Cryogenic Oven Trapping." Journal of AOAC International, vol. 84, no. 1, 2001, pp. 19-23.
Kondo K, Lee XP, Kumazawa T, et al. Sensitive determination of n-hexane and cyclohexane in human body fluids by capillary gas chromatography with cryogenic oven trapping. J AOAC Int. 2001;84(1):19-23.
Kondo, K., Lee, X. P., Kumazawa, T., Sato, K., Watanabe-Suzuki, K., Seno, H., & Suzuki, O. (2001). Sensitive determination of n-hexane and cyclohexane in human body fluids by capillary gas chromatography with cryogenic oven trapping. Journal of AOAC International, 84(1), 19-23.
Kondo K, et al. Sensitive Determination of N-hexane and Cyclohexane in Human Body Fluids By Capillary Gas Chromatography With Cryogenic Oven Trapping. J AOAC Int. 2001 Jan-Feb;84(1):19-23. PubMed PMID: 11234809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of n-hexane and cyclohexane in human body fluids by capillary gas chromatography with cryogenic oven trapping. AU - Kondo,K, AU - Lee,X P, AU - Kumazawa,T, AU - Sato,K, AU - Watanabe-Suzuki,K, AU - Seno,H, AU - Suzuki,O, PY - 2001/3/10/pubmed PY - 2001/6/2/medline PY - 2001/3/10/entrez SP - 19 EP - 23 JF - Journal of AOAC International JO - J AOAC Int VL - 84 IS - 1 N2 - A sensitive method was developed for determination of n-hexane and cyclohexane in human body fluids by headspace capillary gas chromatography (GC) with cryogenic oven trapping. Whole blood and urine samples containing n-hexane and cyclohexane were heated in a 7.5 mL vial at 70 degrees C for 15 min, and 5 mL of the headspace vapor was drawn into a glass syringe. All vapor was introduced through an injection port of a GC instrument in the splitless mode into an Rtx-Volatiles middle-bore capillary column at an oven temperature of -40 degrees C for trapping volatile compounds. The oven temperature was programmed to 180 degrees C for GC with flame ionization detection. These conditions gave sharp peaks for both n-hexane and cyclohexane, a good separation of each peak, and low background impurities for whole blood and urine. The extraction efficiencies of n-hexane and cyclohexane were 13.2-30.3% for whole blood and 12.7-20.7% for urine. The coefficients of within-day variation in terms of extraction efficiency of both compounds were 5.0-9.5% for whole blood and 3.8-10.8% for urine; those of day-to-day variation for the compounds were not greater than 16.6%. The regression equations for n-hexane and cyclohexane showed good linearity in the range of 5-500 ng/0.5 mL for whole blood and urine. The detection limits (signal-to-noise ratio = 3) for both compounds were 1.2 and 0.5 ng/0.5 mL for whole blood and urine, respectively. The data on n-hexane or cyclohexane in rat blood after inhalation of each compound are also presented. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/11234809/Sensitive_determination_of_n_hexane_and_cyclohexane_in_human_body_fluids_by_capillary_gas_chromatography_with_cryogenic_oven_trapping_ DB - PRIME DP - Unbound Medicine ER -