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[Partial nucleotide sequences of hepatitis E viruses isolated from patients in 14 cities of China].
Zhonghua Yi Xue Za Zhi. 2000 Dec; 80(12):893-6.ZY

Abstract

OBJECTIVE

To investigate the genotypes of hepatitis E viruses isolated in China.

METHODS

The partial genome of open reading frame 2 (nt6461-6860, nt5994-6294) of 45 HEV isolated from patients in 14 cities of China was amplified and sequenced using polymerase chain reaction and direct sequencing.

RESULTS

41 of 45 isolates (91%) shared the same genotype with HEV Burma strain (B), with nucleotide identities higher than 98% with the representative HEV Chinese strain. Only 4 HEV isolates were significantly divergent from the 3 prototype strains of HEV, with nucleotide identities of 77%-80% with HEV Burmese/Chinese strain, 74%-76% with Mexico strain and 74%-77% with the newly discovered HEV US/Swine strain, respectively. Phylogenetic analysis suggested that these 4 isolates represented 2 different subtypes belonging to a novel genotype of HEV, which was significantly divergent from the prototype Mexican, Burmese and US/Swine isolates.

CONCLUSION

Among patients with hepatitis E in China, most are infected with the Chinese prototype HEV, and only a small part with the new genotype HEV.

Authors+Show Affiliations

Department of Microbiology, Beijing University, Beijing 100083, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

11236627

Citation

Zhuang, H, et al. "[Partial Nucleotide Sequences of Hepatitis E Viruses Isolated From Patients in 14 Cities of China]." Zhonghua Yi Xue Za Zhi, vol. 80, no. 12, 2000, pp. 893-6.
Zhuang H, Li K, Zhu W. [Partial nucleotide sequences of hepatitis E viruses isolated from patients in 14 cities of China]. Zhonghua Yi Xue Za Zhi. 2000;80(12):893-6.
Zhuang, H., Li, K., & Zhu, W. (2000). [Partial nucleotide sequences of hepatitis E viruses isolated from patients in 14 cities of China]. Zhonghua Yi Xue Za Zhi, 80(12), 893-6.
Zhuang H, Li K, Zhu W. [Partial Nucleotide Sequences of Hepatitis E Viruses Isolated From Patients in 14 Cities of China]. Zhonghua Yi Xue Za Zhi. 2000;80(12):893-6. PubMed PMID: 11236627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Partial nucleotide sequences of hepatitis E viruses isolated from patients in 14 cities of China]. AU - Zhuang,H, AU - Li,K, AU - Zhu,W, PY - 2001/3/10/pubmed PY - 2002/10/3/medline PY - 2001/3/10/entrez SP - 893 EP - 6 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 80 IS - 12 N2 - OBJECTIVE: To investigate the genotypes of hepatitis E viruses isolated in China. METHODS: The partial genome of open reading frame 2 (nt6461-6860, nt5994-6294) of 45 HEV isolated from patients in 14 cities of China was amplified and sequenced using polymerase chain reaction and direct sequencing. RESULTS: 41 of 45 isolates (91%) shared the same genotype with HEV Burma strain (B), with nucleotide identities higher than 98% with the representative HEV Chinese strain. Only 4 HEV isolates were significantly divergent from the 3 prototype strains of HEV, with nucleotide identities of 77%-80% with HEV Burmese/Chinese strain, 74%-76% with Mexico strain and 74%-77% with the newly discovered HEV US/Swine strain, respectively. Phylogenetic analysis suggested that these 4 isolates represented 2 different subtypes belonging to a novel genotype of HEV, which was significantly divergent from the prototype Mexican, Burmese and US/Swine isolates. CONCLUSION: Among patients with hepatitis E in China, most are infected with the Chinese prototype HEV, and only a small part with the new genotype HEV. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/11236627/[Partial_nucleotide_sequences_of_hepatitis_E_viruses_isolated_from_patients_in_14_cities_of_China]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2000&vol=80&issue=12&fpage=893 DB - PRIME DP - Unbound Medicine ER -