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Neurochemical characteristics associated with ethanol preference in selected alcohol-preferring and -nonpreferring rats: a quantitative microdialysis study.
Alcohol Clin Exp Res. 2001 Feb; 25(2):198-205.AC

Abstract

BACKGROUND

Rodent lines selected for alcohol preference and nonpreference have been used extensively to determine the neurobiological basis of alcohol-seeking behavior. Evidence suggests that innate differences in the mesolimbic dopamine and serotonin systems may contribute to disparate alcohol-seeking behaviors between these selected lines. Therefore, the purpose of this study was to identify neurochemical characteristics which may predict ethanol preference in selected alcohol-preferring and -nonpreferring rats [high-alcohol-preferring (HAD), low-alcohol-preferring (LAD), Alko alcohol (AA), Alko nonalcohol (ANA), Wistar].

METHODS

Basal release of dopamine (DA) and serotonin (5-HT) in the nucleus accumbens of ethanol-naive rats was analyzed for its relationship with subsequent measures of ethanol preference. Initially, basal extracellular DA and 5-HT levels were measured by "no-net-flux" quantitative microdialysis. Subsequently, the dopaminergic response to systemic ethanol administration (1.5 g/kg; intraperitoneal) was determined. After completion of the neurochemical tests, the rats received unlimited two-bottle, free-choice access to 10% (w/v) ethanol and water in the home cage for 28 days.

RESULTS

Analysis of the data across individual animals revealed that extracellular dopamine levels ([DA]e; r = + 0.64;p < 0.006) and the percent of baseline increase in DA (%incrDA; r = + 0.77;p < 0.001) due to ethanol were significant predictors of ethanol preference. Comparison of the data between genetic lines yielded a significant relationship between preference and %incrDA (r = + 0.87; p < 0.05). Analysis of the data across animals within each line and their respective control line determined that in the AA/ANA line pair (r = + 0.67; p < 0.03) and Wistar line (r = + 0.66; p < 0.03) %incrDA was a significant predictor of preference. In the HAD/LAD line pair, %incrDA (r = + 0.56; p < 0.005) and [DA]e (r = + 0.86; p < 0.004) were significant predictors of ethanol preference.

CONCLUSIONS

Overall, these findings suggest that elevated extracellular levels of dopamine within the nucleus accumbens and a greater responsivity to enhancements in DA release by ethanol may be factors which contribute to high-alcohol preference. Furthermore, the data suggest that alcohol may be more reinforcing in animals that exhibit an enhanced dopaminergic response to the first ethanol exposure, and that this effect may subsequently be associated with high-alcohol-seeking behavior.

Authors+Show Affiliations

Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California, USA. skatner@yahoo.comNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11236833

Citation

Katner, S N., and F Weiss. "Neurochemical Characteristics Associated With Ethanol Preference in Selected Alcohol-preferring and -nonpreferring Rats: a Quantitative Microdialysis Study." Alcoholism, Clinical and Experimental Research, vol. 25, no. 2, 2001, pp. 198-205.
Katner SN, Weiss F. Neurochemical characteristics associated with ethanol preference in selected alcohol-preferring and -nonpreferring rats: a quantitative microdialysis study. Alcohol Clin Exp Res. 2001;25(2):198-205.
Katner, S. N., & Weiss, F. (2001). Neurochemical characteristics associated with ethanol preference in selected alcohol-preferring and -nonpreferring rats: a quantitative microdialysis study. Alcoholism, Clinical and Experimental Research, 25(2), 198-205.
Katner SN, Weiss F. Neurochemical Characteristics Associated With Ethanol Preference in Selected Alcohol-preferring and -nonpreferring Rats: a Quantitative Microdialysis Study. Alcohol Clin Exp Res. 2001;25(2):198-205. PubMed PMID: 11236833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurochemical characteristics associated with ethanol preference in selected alcohol-preferring and -nonpreferring rats: a quantitative microdialysis study. AU - Katner,S N, AU - Weiss,F, PY - 2001/3/10/pubmed PY - 2001/5/1/medline PY - 2001/3/10/entrez SP - 198 EP - 205 JF - Alcoholism, clinical and experimental research JO - Alcohol Clin Exp Res VL - 25 IS - 2 N2 - BACKGROUND: Rodent lines selected for alcohol preference and nonpreference have been used extensively to determine the neurobiological basis of alcohol-seeking behavior. Evidence suggests that innate differences in the mesolimbic dopamine and serotonin systems may contribute to disparate alcohol-seeking behaviors between these selected lines. Therefore, the purpose of this study was to identify neurochemical characteristics which may predict ethanol preference in selected alcohol-preferring and -nonpreferring rats [high-alcohol-preferring (HAD), low-alcohol-preferring (LAD), Alko alcohol (AA), Alko nonalcohol (ANA), Wistar]. METHODS: Basal release of dopamine (DA) and serotonin (5-HT) in the nucleus accumbens of ethanol-naive rats was analyzed for its relationship with subsequent measures of ethanol preference. Initially, basal extracellular DA and 5-HT levels were measured by "no-net-flux" quantitative microdialysis. Subsequently, the dopaminergic response to systemic ethanol administration (1.5 g/kg; intraperitoneal) was determined. After completion of the neurochemical tests, the rats received unlimited two-bottle, free-choice access to 10% (w/v) ethanol and water in the home cage for 28 days. RESULTS: Analysis of the data across individual animals revealed that extracellular dopamine levels ([DA]e; r = + 0.64;p < 0.006) and the percent of baseline increase in DA (%incrDA; r = + 0.77;p < 0.001) due to ethanol were significant predictors of ethanol preference. Comparison of the data between genetic lines yielded a significant relationship between preference and %incrDA (r = + 0.87; p < 0.05). Analysis of the data across animals within each line and their respective control line determined that in the AA/ANA line pair (r = + 0.67; p < 0.03) and Wistar line (r = + 0.66; p < 0.03) %incrDA was a significant predictor of preference. In the HAD/LAD line pair, %incrDA (r = + 0.56; p < 0.005) and [DA]e (r = + 0.86; p < 0.004) were significant predictors of ethanol preference. CONCLUSIONS: Overall, these findings suggest that elevated extracellular levels of dopamine within the nucleus accumbens and a greater responsivity to enhancements in DA release by ethanol may be factors which contribute to high-alcohol preference. Furthermore, the data suggest that alcohol may be more reinforcing in animals that exhibit an enhanced dopaminergic response to the first ethanol exposure, and that this effect may subsequently be associated with high-alcohol-seeking behavior. SN - 0145-6008 UR - https://www.unboundmedicine.com/medline/citation/11236833/Neurochemical_characteristics_associated_with_ethanol_preference_in_selected_alcohol_preferring_and__nonpreferring_rats:_a_quantitative_microdialysis_study_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=11236833.ui DB - PRIME DP - Unbound Medicine ER -