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Delta-aminolevulinate dehydratase polymorphism and blood lead levels in Chinese children.
Environ Res. 2001 Mar; 85(3):185-90.ER

Abstract

This study investigated the relationship between the delta-aminolevulinate dehydratase (ALAD) isozymes and the blood lead levels of Chinese children. The purpose of this study was to determine the precise ALAD genotyping in Chinese children and identify the contribution of the ALAD genotype to the body lead burden. Blood samples were obtained from 109 boys and 120 girls. These children were 6-10 years old and from a single primary school. Both the school and their homes were within a community in which a large smelter was located. An environmental questionnaire was obtained for each subject, and blood lead levels and ALAD isozyme phenotype were analyzed in a double-blinded fashion. The blood lead levels of 229 children ranged from 4.5 to 26.4 microg/dl; the mean was 10.3 microg/dl and the standard deviation was 3.3 microg/dl. The gene distribution of the ALAD isozyme phenotypes in these environmentally exposed children was ALAD 1-1 (92%), ALAD 1-2, (8%), and ALAD 2-2 (0%). The mean blood level of the environmentally exposed children, who were homozygous for the ALAD1 allele, was 9.7 microg/dl; the mean for those who were heterozygous for the ALAD2 allele was 11.7 microg/dl. Using the t test, the means of the groups were different at the level of t=2.2058, P<0.05. Step-wise regression and multiple analyses of covariance were employed to control the confounders to measuring the independent contribution of the ALAD genotype on blood lead levels. After controlling the confounders, the contribution of the ALAD genotype to the blood lead level was greater and still statistically significant (F=7.3201, P<0.01). These results indicate that individuals carrying the ALAD2 allele are more likely to have sustained increases in blood lead levels when exposed to a lead-contaminated environment.

Authors+Show Affiliations

Shen XM
Research Center for Childhood Lead Poisoning Prevention, Xin-Hua Hospital, Shanghai Second Medical University, 1665 Kong Jiang Road, Shanghai, 200092, China.
Wu SH
No affiliation info available
Yan CH
No affiliation info available
Zhao W
No affiliation info available
Ao LM
No affiliation info available
Zhang YW
No affiliation info available
He JM
No affiliation info available
Ying JM
No affiliation info available
Li RQ
No affiliation info available
Wu SM
No affiliation info available
Guo D
No affiliation info available

MeSH

AllelesChildChinaFemaleHumansIsoenzymesLeadMalePhenotypePolymorphism, GeneticPorphobilinogen Synthase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11237505

Citation

Shen, X M., et al. "Delta-aminolevulinate Dehydratase Polymorphism and Blood Lead Levels in Chinese Children." Environmental Research, vol. 85, no. 3, 2001, pp. 185-90.
Shen XM, Wu SH, Yan CH, et al. Delta-aminolevulinate dehydratase polymorphism and blood lead levels in Chinese children. Environ Res. 2001;85(3):185-90.
Shen, X. M., Wu, S. H., Yan, C. H., Zhao, W., Ao, L. M., Zhang, Y. W., He, J. M., Ying, J. M., Li, R. Q., Wu, S. M., & Guo, D. (2001). Delta-aminolevulinate dehydratase polymorphism and blood lead levels in Chinese children. Environmental Research, 85(3), 185-90.
Shen XM, et al. Delta-aminolevulinate Dehydratase Polymorphism and Blood Lead Levels in Chinese Children. Environ Res. 2001;85(3):185-90. PubMed PMID: 11237505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Delta-aminolevulinate dehydratase polymorphism and blood lead levels in Chinese children. AU - Shen,X M, AU - Wu,S H, AU - Yan,C H, AU - Zhao,W, AU - Ao,L M, AU - Zhang,Y W, AU - He,J M, AU - Ying,J M, AU - Li,R Q, AU - Wu,S M, AU - Guo,D, PY - 2001/3/10/pubmed PY - 2001/4/6/medline PY - 2001/3/10/entrez SP - 185 EP - 90 JF - Environmental research JO - Environ Res VL - 85 IS - 3 N2 - This study investigated the relationship between the delta-aminolevulinate dehydratase (ALAD) isozymes and the blood lead levels of Chinese children. The purpose of this study was to determine the precise ALAD genotyping in Chinese children and identify the contribution of the ALAD genotype to the body lead burden. Blood samples were obtained from 109 boys and 120 girls. These children were 6-10 years old and from a single primary school. Both the school and their homes were within a community in which a large smelter was located. An environmental questionnaire was obtained for each subject, and blood lead levels and ALAD isozyme phenotype were analyzed in a double-blinded fashion. The blood lead levels of 229 children ranged from 4.5 to 26.4 microg/dl; the mean was 10.3 microg/dl and the standard deviation was 3.3 microg/dl. The gene distribution of the ALAD isozyme phenotypes in these environmentally exposed children was ALAD 1-1 (92%), ALAD 1-2, (8%), and ALAD 2-2 (0%). The mean blood level of the environmentally exposed children, who were homozygous for the ALAD1 allele, was 9.7 microg/dl; the mean for those who were heterozygous for the ALAD2 allele was 11.7 microg/dl. Using the t test, the means of the groups were different at the level of t=2.2058, P<0.05. Step-wise regression and multiple analyses of covariance were employed to control the confounders to measuring the independent contribution of the ALAD genotype on blood lead levels. After controlling the confounders, the contribution of the ALAD genotype to the blood lead level was greater and still statistically significant (F=7.3201, P<0.01). These results indicate that individuals carrying the ALAD2 allele are more likely to have sustained increases in blood lead levels when exposed to a lead-contaminated environment. SN - 0013-9351 UR - https://www.unboundmedicine.com/medline/citation/11237505/Delta_aminolevulinate_dehydratase_polymorphism_and_blood_lead_levels_in_Chinese_children_ DB - PRIME DP - Unbound Medicine ER -