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Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break.
Mol Cell Biol. 2001 Mar; 21(6):2048-56.MC

Abstract

Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence of RAD52, repair is nearly absent and diploid cells lose the broken chromosome; however, in cells lacking RAD51, gene conversion is absent but cells can repair the DSB by BIR. We now report that gene conversion is also abolished when RAD54, RAD55, and RAD57 are deleted but BIR occurs, as with rad51Delta cells. DSB-induced gene conversion is not significantly affected when RAD50, RAD59, TID1 (RDH54), SRS2, or SGS1 is deleted. Various double mutations largely eliminate both gene conversion and BIR, including rad51Delta rad50Delta, rad51Delta rad59Delta, and rad54Delta tid1Delta. These results demonstrate that there is a RAD51- and RAD54-independent BIR pathway that requires RAD59, TID1, RAD50, and presumably MRE11 and XRS2. The similar genetic requirements for BIR and telomere maintenance in the absence of telomerase also suggest that these two processes proceed by similar mechanisms.

Authors+Show Affiliations

Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11238940

Citation

Signon, L, et al. "Genetic Requirements for RAD51- and RAD54-independent Break-induced Replication Repair of a Chromosomal Double-strand Break." Molecular and Cellular Biology, vol. 21, no. 6, 2001, pp. 2048-56.
Signon L, Malkova A, Naylor ML, et al. Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. Mol Cell Biol. 2001;21(6):2048-56.
Signon, L., Malkova, A., Naylor, M. L., Klein, H., & Haber, J. E. (2001). Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. Molecular and Cellular Biology, 21(6), 2048-56.
Signon L, et al. Genetic Requirements for RAD51- and RAD54-independent Break-induced Replication Repair of a Chromosomal Double-strand Break. Mol Cell Biol. 2001;21(6):2048-56. PubMed PMID: 11238940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. AU - Signon,L, AU - Malkova,A, AU - Naylor,M L, AU - Klein,H, AU - Haber,J E, PY - 2001/3/10/pubmed PY - 2001/4/21/medline PY - 2001/3/10/entrez SP - 2048 EP - 56 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 21 IS - 6 N2 - Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence of RAD52, repair is nearly absent and diploid cells lose the broken chromosome; however, in cells lacking RAD51, gene conversion is absent but cells can repair the DSB by BIR. We now report that gene conversion is also abolished when RAD54, RAD55, and RAD57 are deleted but BIR occurs, as with rad51Delta cells. DSB-induced gene conversion is not significantly affected when RAD50, RAD59, TID1 (RDH54), SRS2, or SGS1 is deleted. Various double mutations largely eliminate both gene conversion and BIR, including rad51Delta rad50Delta, rad51Delta rad59Delta, and rad54Delta tid1Delta. These results demonstrate that there is a RAD51- and RAD54-independent BIR pathway that requires RAD59, TID1, RAD50, and presumably MRE11 and XRS2. The similar genetic requirements for BIR and telomere maintenance in the absence of telomerase also suggest that these two processes proceed by similar mechanisms. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/11238940/Genetic_requirements_for_RAD51__and_RAD54_independent_break_induced_replication_repair_of_a_chromosomal_double_strand_break_ L2 - https://journals.asm.org/doi/10.1128/MCB.21.6.2048-2056.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -