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The fine specificity of the myelin oligodendrocyte glycoprotein autoantibody response in patients with multiple sclerosis and normal healthy controls.
J Neuroimmunol 2001; 114(1-2):220-5JN

Abstract

Antibodies directed against the extracellular immunoglobulin (Ig)-like domain of the myelin oligodendrocyte glycoprotein (MOG(Igd)) mediate demyelination in experimental autoimmune encephalomyelitis (EAE) and are implicated in the immunopathogenesis of multiple sclerosis (MS). In this study we investigated the epitope specificity of MOG(Igd)-specific autoantibodies immunopurified from MS patients (n=17) and normal healthy controls (HD; n=9). ELISA, using a panel of synthetic MOG(Igd) peptides, revealed that the epitope specificity of this response was heterogeneous in both groups. The most frequently recognised epitopes were located in amino acid sequences (a.a.) 1-26 (13/17) and 63-87 (15/17) in MS patients, and 14-39 (6/9) and 63-87 (6/9) in HDs, but there was no association between MS and any particular peptide specificity. We therefore investigated the ability of the immunopurified antibodies to recognise native MOG(Igd) expressed on at the membrane surface by FACS. Unexpectedly, antibodies fulfilling this essential criterion for a demyelinating antibody response were detected only in one of the MS samples. These results indicate that the epitope specificity of the human B cell response to MOG is not only heterogeneous, but may only mediate demyelination in a limited subset of MS patients.

Authors+Show Affiliations

Department of Neuroimmunology, Max-Planck Institute of Neurobiology, Am Klopferspitz 18a; 82152, Martinsried, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11240035

Citation

Haase, C G., et al. "The Fine Specificity of the Myelin Oligodendrocyte Glycoprotein Autoantibody Response in Patients With Multiple Sclerosis and Normal Healthy Controls." Journal of Neuroimmunology, vol. 114, no. 1-2, 2001, pp. 220-5.
Haase CG, Guggenmos J, Brehm U, et al. The fine specificity of the myelin oligodendrocyte glycoprotein autoantibody response in patients with multiple sclerosis and normal healthy controls. J Neuroimmunol. 2001;114(1-2):220-5.
Haase, C. G., Guggenmos, J., Brehm, U., Andersson, M., Olsson, T., Reindl, M., ... Linington, C. (2001). The fine specificity of the myelin oligodendrocyte glycoprotein autoantibody response in patients with multiple sclerosis and normal healthy controls. Journal of Neuroimmunology, 114(1-2), pp. 220-5.
Haase CG, et al. The Fine Specificity of the Myelin Oligodendrocyte Glycoprotein Autoantibody Response in Patients With Multiple Sclerosis and Normal Healthy Controls. J Neuroimmunol. 2001 Mar 1;114(1-2):220-5. PubMed PMID: 11240035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fine specificity of the myelin oligodendrocyte glycoprotein autoantibody response in patients with multiple sclerosis and normal healthy controls. AU - Haase,C G, AU - Guggenmos,J, AU - Brehm,U, AU - Andersson,M, AU - Olsson,T, AU - Reindl,M, AU - Schneidewind,J M, AU - Zettl,U K, AU - Heidenreich,F, AU - Berger,T, AU - Wekerle,H, AU - Hohlfeld,R, AU - Linington,C, PY - 2001/3/10/pubmed PY - 2001/4/17/medline PY - 2001/3/10/entrez SP - 220 EP - 5 JF - Journal of neuroimmunology JO - J. Neuroimmunol. VL - 114 IS - 1-2 N2 - Antibodies directed against the extracellular immunoglobulin (Ig)-like domain of the myelin oligodendrocyte glycoprotein (MOG(Igd)) mediate demyelination in experimental autoimmune encephalomyelitis (EAE) and are implicated in the immunopathogenesis of multiple sclerosis (MS). In this study we investigated the epitope specificity of MOG(Igd)-specific autoantibodies immunopurified from MS patients (n=17) and normal healthy controls (HD; n=9). ELISA, using a panel of synthetic MOG(Igd) peptides, revealed that the epitope specificity of this response was heterogeneous in both groups. The most frequently recognised epitopes were located in amino acid sequences (a.a.) 1-26 (13/17) and 63-87 (15/17) in MS patients, and 14-39 (6/9) and 63-87 (6/9) in HDs, but there was no association between MS and any particular peptide specificity. We therefore investigated the ability of the immunopurified antibodies to recognise native MOG(Igd) expressed on at the membrane surface by FACS. Unexpectedly, antibodies fulfilling this essential criterion for a demyelinating antibody response were detected only in one of the MS samples. These results indicate that the epitope specificity of the human B cell response to MOG is not only heterogeneous, but may only mediate demyelination in a limited subset of MS patients. SN - 0165-5728 UR - https://www.unboundmedicine.com/medline/citation/11240035/The_fine_specificity_of_the_myelin_oligodendrocyte_glycoprotein_autoantibody_response_in_patients_with_multiple_sclerosis_and_normal_healthy_controls_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165572800004628 DB - PRIME DP - Unbound Medicine ER -