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In situ localization of beta-glucans in the cell wall of Schizosaccharomyces pombe.
Yeast. 2001 Mar 30; 18(5):433-44.Y

Abstract

The chemical composition of the cell wall of Sz. pombe is known as beta-1,3-glucan, beta-1,6-glucan, alpha-1,3-glucan and alpha-galactomannan; however, the three-dimensional interactions of those macromolecules have not yet been clarified. Transmission electron microscopy reveals a three-layered structure: the outer layer is electron-dense, the adjacent layer is less dense, and the third layer bordering the cell membrane is dense. In intact cells of Sz. pombe, the high-resolution scanning electron microscope reveals a surface completely filled with alpha-galactomannan particles. To better understand the organization of the cell wall and to complement our previous studies, we set out to locate the three different types of beta-glucan by immuno-electron microscopy. Our results suggest that the less dense layer of the cell wall contains mainly beta-1,6-branched beta-1,3-glucan. Occasionally a line of gold particles can be seen, labelling fine filaments radiating from the cell membrane to the alpha-galactomannan layer, suggesting that some of the radial filaments contain beta-1,6-branched beta-1,3-glucan. beta-1,6-glucan is preferentially located underneath the alpha-galactomannan layer. Linear beta-1,3-glucan is exclusively located in the primary septum of dividing cells. beta-1,6-glucan only labels the secondary septum and does not co-localize with linear beta-1,3-glucan, while beta-1,6-branched beta-1,3-glucan is present in both septa. Linear beta-1,3-glucan is present from early stages of septum formation and persists until the septum is completely formed; then just before cell division the label disappears. From these results we suggest that linear beta-1,3-glucan is involved in septum formation and perhaps the separation of the two daughter cells. In addition, we frequently found beta-1,6-glucan label on the Golgi apparatus, on small vesicles and underneath the cell membrane. These results give fresh evidence for the hypothesis that beta-1,6-glucan is synthesized in the endoplasmic reticulum-Golgi system and exported to the cell membrane.

Authors+Show Affiliations

Humbel BM
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan. bruno@accu.uu.nl
Konomi M
No affiliation info available
Takagi T
No affiliation info available
Kamasawa N
No affiliation info available
Ishijima SA
No affiliation info available
Osumi M
No affiliation info available

MeSH

Cell WallGlucansGolgi ApparatusMicroscopy, ImmunoelectronSchizosaccharomyces

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11255251

Citation

Humbel, B M., et al. "In Situ Localization of Beta-glucans in the Cell Wall of Schizosaccharomyces Pombe." Yeast (Chichester, England), vol. 18, no. 5, 2001, pp. 433-44.
Humbel BM, Konomi M, Takagi T, et al. In situ localization of beta-glucans in the cell wall of Schizosaccharomyces pombe. Yeast. 2001;18(5):433-44.
Humbel, B. M., Konomi, M., Takagi, T., Kamasawa, N., Ishijima, S. A., & Osumi, M. (2001). In situ localization of beta-glucans in the cell wall of Schizosaccharomyces pombe. Yeast (Chichester, England), 18(5), 433-44.
Humbel BM, et al. In Situ Localization of Beta-glucans in the Cell Wall of Schizosaccharomyces Pombe. Yeast. 2001 Mar 30;18(5):433-44. PubMed PMID: 11255251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ localization of beta-glucans in the cell wall of Schizosaccharomyces pombe. AU - Humbel,B M, AU - Konomi,M, AU - Takagi,T, AU - Kamasawa,N, AU - Ishijima,S A, AU - Osumi,M, PY - 2001/3/20/pubmed PY - 2001/6/22/medline PY - 2001/3/20/entrez SP - 433 EP - 44 JF - Yeast (Chichester, England) JO - Yeast VL - 18 IS - 5 N2 - The chemical composition of the cell wall of Sz. pombe is known as beta-1,3-glucan, beta-1,6-glucan, alpha-1,3-glucan and alpha-galactomannan; however, the three-dimensional interactions of those macromolecules have not yet been clarified. Transmission electron microscopy reveals a three-layered structure: the outer layer is electron-dense, the adjacent layer is less dense, and the third layer bordering the cell membrane is dense. In intact cells of Sz. pombe, the high-resolution scanning electron microscope reveals a surface completely filled with alpha-galactomannan particles. To better understand the organization of the cell wall and to complement our previous studies, we set out to locate the three different types of beta-glucan by immuno-electron microscopy. Our results suggest that the less dense layer of the cell wall contains mainly beta-1,6-branched beta-1,3-glucan. Occasionally a line of gold particles can be seen, labelling fine filaments radiating from the cell membrane to the alpha-galactomannan layer, suggesting that some of the radial filaments contain beta-1,6-branched beta-1,3-glucan. beta-1,6-glucan is preferentially located underneath the alpha-galactomannan layer. Linear beta-1,3-glucan is exclusively located in the primary septum of dividing cells. beta-1,6-glucan only labels the secondary septum and does not co-localize with linear beta-1,3-glucan, while beta-1,6-branched beta-1,3-glucan is present in both septa. Linear beta-1,3-glucan is present from early stages of septum formation and persists until the septum is completely formed; then just before cell division the label disappears. From these results we suggest that linear beta-1,3-glucan is involved in septum formation and perhaps the separation of the two daughter cells. In addition, we frequently found beta-1,6-glucan label on the Golgi apparatus, on small vesicles and underneath the cell membrane. These results give fresh evidence for the hypothesis that beta-1,6-glucan is synthesized in the endoplasmic reticulum-Golgi system and exported to the cell membrane. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/11255251/In_situ_localization_of_beta_glucans_in_the_cell_wall_of_Schizosaccharomyces_pombe_ DB - PRIME DP - Unbound Medicine ER -