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Leptospirosis in Kuala Lumpur and the comparative evaluation of two rapid commercial diagnostic kits against the MAT test for the detection of antibodies to leptospira interrogans.
Singapore Med J. 2000 Aug; 41(8):370-5.SM

Abstract

The aim of the study was to look into the epidemiology of serodiagnosed cases of leptospirosis at the University Hospital and compare two commercial ELISA Assays to the Microscopic Agglutination Test (MAT). Demographic data for all serodiagnosed cases for the years 1991-1997 were collected. From this data, 104 sera (n = 104) were selected as samples for comparative evaluation of the commercial ELISAs (INDX Dip-S-Ticks and PanBio ELISA) to the MAT test. Thirty two (n = 32) negative control sera were selected from serodiagnosed cases of other differential diagnosis of leptospira infection. The MAT test is a standard test that detects agglutination antibodies to leptospira biflexa, while the INDX Dip-S-Ticks is an ELISA dot test assaying for total anti-leptospira antibodies. The PanBio ELISA is a colorometric assay in test well strips to detect anti-leptospira IgM. The sensitivity, specificity, and efficiency of tests were calculated at a MAT cut-off value of 1:320. Demographic data showed that leptospirosis peaks during March-May and Aug-Nov coinciding with the inter-monsoon period with more men being infected than women and more adults than children. The sensitivity, specificity, and efficiency of test for the INDX Dip-S-Ticks were 83.3%, 93.8% and 87.5% while the values for the PanBio ELISA were 54.2%, 96.9% and 71.3%. The suboptimal PanBio result could be related to the blocking effect of high IgG titres or could be related to the diagnostic MAT cut-off values used in this study. The data hence reflects a pattern of transmission that is related to "wet" occupational risk factors. The commercial assays evaluated, are easier to perform but interpretation of results should be based on level of endemicity. The INDX Dip-S-Ticks allows this flexibility and is a practical alternative to the MAT test.

Authors+Show Affiliations

Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603 Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Validation Study

Language

eng

PubMed ID

11256343

Citation

Sekhar, W Y., et al. "Leptospirosis in Kuala Lumpur and the Comparative Evaluation of Two Rapid Commercial Diagnostic Kits Against the MAT Test for the Detection of Antibodies to Leptospira Interrogans." Singapore Medical Journal, vol. 41, no. 8, 2000, pp. 370-5.
Sekhar WY, Soo EH, Gopalakrishnan V, et al. Leptospirosis in Kuala Lumpur and the comparative evaluation of two rapid commercial diagnostic kits against the MAT test for the detection of antibodies to leptospira interrogans. Singapore Med J. 2000;41(8):370-5.
Sekhar, W. Y., Soo, E. H., Gopalakrishnan, V., & Devi, S. (2000). Leptospirosis in Kuala Lumpur and the comparative evaluation of two rapid commercial diagnostic kits against the MAT test for the detection of antibodies to leptospira interrogans. Singapore Medical Journal, 41(8), 370-5.
Sekhar WY, et al. Leptospirosis in Kuala Lumpur and the Comparative Evaluation of Two Rapid Commercial Diagnostic Kits Against the MAT Test for the Detection of Antibodies to Leptospira Interrogans. Singapore Med J. 2000;41(8):370-5. PubMed PMID: 11256343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leptospirosis in Kuala Lumpur and the comparative evaluation of two rapid commercial diagnostic kits against the MAT test for the detection of antibodies to leptospira interrogans. AU - Sekhar,W Y, AU - Soo,E H, AU - Gopalakrishnan,V, AU - Devi,S, PY - 2001/3/21/pubmed PY - 2001/5/22/medline PY - 2001/3/21/entrez SP - 370 EP - 5 JF - Singapore medical journal JO - Singapore Med J VL - 41 IS - 8 N2 - The aim of the study was to look into the epidemiology of serodiagnosed cases of leptospirosis at the University Hospital and compare two commercial ELISA Assays to the Microscopic Agglutination Test (MAT). Demographic data for all serodiagnosed cases for the years 1991-1997 were collected. From this data, 104 sera (n = 104) were selected as samples for comparative evaluation of the commercial ELISAs (INDX Dip-S-Ticks and PanBio ELISA) to the MAT test. Thirty two (n = 32) negative control sera were selected from serodiagnosed cases of other differential diagnosis of leptospira infection. The MAT test is a standard test that detects agglutination antibodies to leptospira biflexa, while the INDX Dip-S-Ticks is an ELISA dot test assaying for total anti-leptospira antibodies. The PanBio ELISA is a colorometric assay in test well strips to detect anti-leptospira IgM. The sensitivity, specificity, and efficiency of tests were calculated at a MAT cut-off value of 1:320. Demographic data showed that leptospirosis peaks during March-May and Aug-Nov coinciding with the inter-monsoon period with more men being infected than women and more adults than children. The sensitivity, specificity, and efficiency of test for the INDX Dip-S-Ticks were 83.3%, 93.8% and 87.5% while the values for the PanBio ELISA were 54.2%, 96.9% and 71.3%. The suboptimal PanBio result could be related to the blocking effect of high IgG titres or could be related to the diagnostic MAT cut-off values used in this study. The data hence reflects a pattern of transmission that is related to "wet" occupational risk factors. The commercial assays evaluated, are easier to perform but interpretation of results should be based on level of endemicity. The INDX Dip-S-Ticks allows this flexibility and is a practical alternative to the MAT test. SN - 0037-5675 UR - https://www.unboundmedicine.com/medline/citation/11256343/Leptospirosis_in_Kuala_Lumpur_and_the_comparative_evaluation_of_two_rapid_commercial_diagnostic_kits_against_the_MAT_test_for_the_detection_of_antibodies_to_leptospira_interrogans_ L2 - http://www.diseaseinfosearch.org/result/4178 DB - PRIME DP - Unbound Medicine ER -