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EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells.
Br J Cancer. 2001 Mar 23; 84(6):844-50.BJ

Abstract

Catechins are key components of teas that have antiproliferative properties. We investigated the effects of green tea catechins on intracellular signalling and VEGF induction in vitro in serum-deprived HT29 human colon cancer cells and in vivo on the growth of HT29 cells in nude mice. In the in vitro studies, (-)-epigallocatechin gallate (EGCG), the most abundant catechin in green tea extract, inhibited Erk-1 and Erk-2 activation in a dose-dependent manner. However, other tea catechins such as (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC) did not affect Erk-1 or 2 activation at a concentration of 30 microM. EGCG also inhibited the increase of VEGF expression and promoter activity induced by serum starvation. In the in vivo studies, athymic BALB/c nude mice were inoculated subcutaneously with HT29 cells and treated with daily intraperitoneal injections of EC (negative control) or EGCG at 1.5 mg day(-1)mouse(-1)starting 2 days after tumour cell inoculation. Treatment with EGCG inhibited tumour growth (58%), microvessel density (30%), and tumour cell proliferation (27%) and increased tumour cell apoptosis (1.9-fold) and endothelial cell apoptosis (3-fold) relative to the control condition (P< 0.05 for all comparisons). EGCG may exert at least part of its anticancer effect by inhibiting angiogenesis through blocking the induction of VEGF.

Authors+Show Affiliations

Chonnam University Research Institute of Medical Sciences, Chonnam University Medical School, Kwangju, Korea 501-190.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11259102

Citation

Jung, Y D., et al. "EGCG, a Major Component of Green Tea, Inhibits Tumour Growth By Inhibiting VEGF Induction in Human Colon Carcinoma Cells." British Journal of Cancer, vol. 84, no. 6, 2001, pp. 844-50.
Jung YD, Kim MS, Shin BA, et al. EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells. Br J Cancer. 2001;84(6):844-50.
Jung, Y. D., Kim, M. S., Shin, B. A., Chay, K. O., Ahn, B. W., Liu, W., Bucana, C. D., Gallick, G. E., & Ellis, L. M. (2001). EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells. British Journal of Cancer, 84(6), 844-50.
Jung YD, et al. EGCG, a Major Component of Green Tea, Inhibits Tumour Growth By Inhibiting VEGF Induction in Human Colon Carcinoma Cells. Br J Cancer. 2001 Mar 23;84(6):844-50. PubMed PMID: 11259102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells. AU - Jung,Y D, AU - Kim,M S, AU - Shin,B A, AU - Chay,K O, AU - Ahn,B W, AU - Liu,W, AU - Bucana,C D, AU - Gallick,G E, AU - Ellis,L M, PY - 2001/3/22/pubmed PY - 2001/5/5/medline PY - 2001/3/22/entrez SP - 844 EP - 50 JF - British journal of cancer JO - Br J Cancer VL - 84 IS - 6 N2 - Catechins are key components of teas that have antiproliferative properties. We investigated the effects of green tea catechins on intracellular signalling and VEGF induction in vitro in serum-deprived HT29 human colon cancer cells and in vivo on the growth of HT29 cells in nude mice. In the in vitro studies, (-)-epigallocatechin gallate (EGCG), the most abundant catechin in green tea extract, inhibited Erk-1 and Erk-2 activation in a dose-dependent manner. However, other tea catechins such as (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC) did not affect Erk-1 or 2 activation at a concentration of 30 microM. EGCG also inhibited the increase of VEGF expression and promoter activity induced by serum starvation. In the in vivo studies, athymic BALB/c nude mice were inoculated subcutaneously with HT29 cells and treated with daily intraperitoneal injections of EC (negative control) or EGCG at 1.5 mg day(-1)mouse(-1)starting 2 days after tumour cell inoculation. Treatment with EGCG inhibited tumour growth (58%), microvessel density (30%), and tumour cell proliferation (27%) and increased tumour cell apoptosis (1.9-fold) and endothelial cell apoptosis (3-fold) relative to the control condition (P< 0.05 for all comparisons). EGCG may exert at least part of its anticancer effect by inhibiting angiogenesis through blocking the induction of VEGF. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/11259102/EGCG_a_major_component_of_green_tea_inhibits_tumour_growth_by_inhibiting_VEGF_induction_in_human_colon_carcinoma_cells_ L2 - https://doi.org/10.1054/bjoc.2000.1691 DB - PRIME DP - Unbound Medicine ER -