Tags

Type your tag names separated by a space and hit enter

SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit.
FASEB J. 2001 Mar; 15(3):645-58.FJ

Abstract

Hair graying, an age-associated process of unknown etiology, is characterized by a reduced number and activity of hair follicle (HF) melanocytes. Stem cell factor (SCF) and its receptor c-kit are important for melanocyte survival during development, and mutations in these genes result in unpigmented hairs. Here we show that during cyclic HF regeneration in C57BL/6 mice, proliferating, differentiating, and melanin-producing melanocytes express c-kit, whereas presumptive melanocyte precursors do not. SCF overexpression in HF epithelium significantly increases the number and proliferative activity of melanocytes. During the induced hair cycle in C57BL/6 mice, administration of anti-c-kit antibody dose-dependently decreases hair pigmentation and leads to partially depigmented (gray) or fully depigmented (white) hairs, associated with significant decreases in melanocyte proliferation and differentiation, as determined by immunostaining and confocal microscopy. However, in the next hair cycle, the previously treated animals grow fully pigmented hairs with the normal number and distribution of melanocytes. This suggests that melanocyte stem cells are not dependent on SCF/c-kit and when appropriately stimulated can generate melanogenically active melanocytes. Therefore, the blockade of c-kit signaling offers a fully reversible model for hair depigmentation, which might be used for the studies of hair pigmentation disorders.

Authors+Show Affiliations

Botchkareva NV
Department of Dermatology, Boston University School of Medicine, Boston, MA, USA.
Khlgatian M
No affiliation info available
Longley BJ
No affiliation info available
Botchkarev VA
No affiliation info available
Gilchrest BA
No affiliation info available

MeSH

AnimalsAntibodies, MonoclonalEpidermal CellsEpidermisFemaleHair ColorHair FollicleHumansImmunohistochemistryIn Situ Nick-End LabelingKi-67 AntigenMelanocytesMembrane GlycoproteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ConfocalOxidoreductasesProteinsProto-Oncogene Proteins c-kitSignal TransductionStem Cell Factor

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11259383

Citation

Botchkareva, N V., et al. "SCF/c-kit Signaling Is Required for Cyclic Regeneration of the Hair Pigmentation Unit." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 15, no. 3, 2001, pp. 645-58.
Botchkareva NV, Khlgatian M, Longley BJ, et al. SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit. FASEB J. 2001;15(3):645-58.
Botchkareva, N. V., Khlgatian, M., Longley, B. J., Botchkarev, V. A., & Gilchrest, B. A. (2001). SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 15(3), 645-58.
Botchkareva NV, et al. SCF/c-kit Signaling Is Required for Cyclic Regeneration of the Hair Pigmentation Unit. FASEB J. 2001;15(3):645-58. PubMed PMID: 11259383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit. AU - Botchkareva,N V, AU - Khlgatian,M, AU - Longley,B J, AU - Botchkarev,V A, AU - Gilchrest,B A, PY - 2001/3/22/pubmed PY - 2001/4/21/medline PY - 2001/3/22/entrez SP - 645 EP - 58 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J VL - 15 IS - 3 N2 - Hair graying, an age-associated process of unknown etiology, is characterized by a reduced number and activity of hair follicle (HF) melanocytes. Stem cell factor (SCF) and its receptor c-kit are important for melanocyte survival during development, and mutations in these genes result in unpigmented hairs. Here we show that during cyclic HF regeneration in C57BL/6 mice, proliferating, differentiating, and melanin-producing melanocytes express c-kit, whereas presumptive melanocyte precursors do not. SCF overexpression in HF epithelium significantly increases the number and proliferative activity of melanocytes. During the induced hair cycle in C57BL/6 mice, administration of anti-c-kit antibody dose-dependently decreases hair pigmentation and leads to partially depigmented (gray) or fully depigmented (white) hairs, associated with significant decreases in melanocyte proliferation and differentiation, as determined by immunostaining and confocal microscopy. However, in the next hair cycle, the previously treated animals grow fully pigmented hairs with the normal number and distribution of melanocytes. This suggests that melanocyte stem cells are not dependent on SCF/c-kit and when appropriately stimulated can generate melanogenically active melanocytes. Therefore, the blockade of c-kit signaling offers a fully reversible model for hair depigmentation, which might be used for the studies of hair pigmentation disorders. SN - 0892-6638 UR - https://www.unboundmedicine.com/medline/citation/11259383/SCF/c_kit_signaling_is_required_for_cyclic_regeneration_of_the_hair_pigmentation_unit_ DB - PRIME DP - Unbound Medicine ER -