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Effect of acidosis on urine supersaturation and stone formation in genetic hypercalciuric stone-forming rats.
Kidney Int. 2001 Apr; 59(4):1415-23.KI

Abstract

BACKGROUND

We have successively inbred over 45 generations a strain of rats to maximize urine calcium excretion. The rats now consistently excrete 8 to 10 times as much calcium as controls and uniformly form poorly crystalline calcium phosphate kidney stones. In humans with calcium nephrolithiasis, consumption of a diet high in acid precursors is often cited as a risk factor for the development of calcium-based kidney stones; however, the effect of this diet on urinary supersaturation with respect to the common solid phases found in kidney stones has not been determined.

METHODS

To determine the effect of the addition of an acid precursor on urine ion excretion, supersaturation, and stone formation, we fed these genetic hypercalciuric stone-forming (GHS) rats 13 g/day of a 1.2% calcium diet with 0.0, 0.5, 1.0, or 1.5% NH4Cl in the drinking water for 14 weeks (N = 8 for each). Urine was collected and analyzed every two weeks.

RESULTS

As expected, the addition of dietary NH4Cl led to a progressive fall in urine pH and urine citrate, while urine ammonium increased. Urine calcium and phosphorus increased, while urine oxalate fell. Increasing dietary NH4Cl led to a fall in supersaturation with respect to CaHPO4 (brushite) and CaOx and a rise in supersaturation with respect to uric acid. In spite of differences in supersaturation, most rats in each group formed stones that contained calcium phosphate and not calcium oxalate.

CONCLUSIONS

Thus, while the provision of additional dietary acids alters urinary ion excretion and lowers supersaturation with respect to CaHPO4 and CaOx, it does not change the character or rate of stone formation in the GHS rats.

Authors+Show Affiliations

Nephrology Unit, University of Rochester School of Medicine, Rochester, New York 14642, USA. david_bushinsky@urmc.rochester.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11260403

Citation

Bushinsky, D A., et al. "Effect of Acidosis On Urine Supersaturation and Stone Formation in Genetic Hypercalciuric Stone-forming Rats." Kidney International, vol. 59, no. 4, 2001, pp. 1415-23.
Bushinsky DA, Grynpas MD, Asplin JR. Effect of acidosis on urine supersaturation and stone formation in genetic hypercalciuric stone-forming rats. Kidney Int. 2001;59(4):1415-23.
Bushinsky, D. A., Grynpas, M. D., & Asplin, J. R. (2001). Effect of acidosis on urine supersaturation and stone formation in genetic hypercalciuric stone-forming rats. Kidney International, 59(4), 1415-23.
Bushinsky DA, Grynpas MD, Asplin JR. Effect of Acidosis On Urine Supersaturation and Stone Formation in Genetic Hypercalciuric Stone-forming Rats. Kidney Int. 2001;59(4):1415-23. PubMed PMID: 11260403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acidosis on urine supersaturation and stone formation in genetic hypercalciuric stone-forming rats. AU - Bushinsky,D A, AU - Grynpas,M D, AU - Asplin,J R, PY - 2001/3/22/pubmed PY - 2001/6/23/medline PY - 2001/3/22/entrez SP - 1415 EP - 23 JF - Kidney international JO - Kidney Int. VL - 59 IS - 4 N2 - BACKGROUND: We have successively inbred over 45 generations a strain of rats to maximize urine calcium excretion. The rats now consistently excrete 8 to 10 times as much calcium as controls and uniformly form poorly crystalline calcium phosphate kidney stones. In humans with calcium nephrolithiasis, consumption of a diet high in acid precursors is often cited as a risk factor for the development of calcium-based kidney stones; however, the effect of this diet on urinary supersaturation with respect to the common solid phases found in kidney stones has not been determined. METHODS: To determine the effect of the addition of an acid precursor on urine ion excretion, supersaturation, and stone formation, we fed these genetic hypercalciuric stone-forming (GHS) rats 13 g/day of a 1.2% calcium diet with 0.0, 0.5, 1.0, or 1.5% NH4Cl in the drinking water for 14 weeks (N = 8 for each). Urine was collected and analyzed every two weeks. RESULTS: As expected, the addition of dietary NH4Cl led to a progressive fall in urine pH and urine citrate, while urine ammonium increased. Urine calcium and phosphorus increased, while urine oxalate fell. Increasing dietary NH4Cl led to a fall in supersaturation with respect to CaHPO4 (brushite) and CaOx and a rise in supersaturation with respect to uric acid. In spite of differences in supersaturation, most rats in each group formed stones that contained calcium phosphate and not calcium oxalate. CONCLUSIONS: Thus, while the provision of additional dietary acids alters urinary ion excretion and lowers supersaturation with respect to CaHPO4 and CaOx, it does not change the character or rate of stone formation in the GHS rats. SN - 0085-2538 UR - https://www.unboundmedicine.com/medline/citation/11260403/Effect_of_acidosis_on_urine_supersaturation_and_stone_formation_in_genetic_hypercalciuric_stone_forming_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0085-2538(15)47615-X DB - PRIME DP - Unbound Medicine ER -