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Properties of transient outward potassium current and inward rectifier potassium current in immature human atrial myocytes.
Zhongguo Yao Li Xue Bao. 1999 Nov; 20(11):1005-10.ZY

Abstract

AIM

To study the properties of transient outward K+ current (Ito) and inward rectifier K+ current (IKl) in immature human heart.

METHODS

Ito and IKl were recorded using whole-cell patch-clamp technique in atrial myocytes isolated from 12 immature (aged from 6 months to 5 a) human hearts.

RESULTS

Ito was voltage-dependent, activated and inactivated rapidly. The IC50 (95% confidence limits) of 4-AP on Ito was 0.64 (0.48-0.87) mmol.L-1. 4-AP 1 mmol.L-1 shifted V1/2 of activation from (6.6 +/- 2.0) mV to (19.8 +/- 3.0) mV (n = 4-10, P < 0.01). 4-AP 0.3 mmol.L-1 changed V1/2 of inactivation from (-49 +/- 4) mV to (-61.4 +/- 2.1) mV (n = 3, P < 0.01), but there were no obvious influence on voltage-dependent activation of Ito (P > 0.05). At the same concentration, the recovery time constant (tau value) was prolonged from (108 +/- 16) ms to (220 +/- 67) ms (n = 3-12, P < 0.01). IKl was also voltage-dependent. Its reverse potential was -40 mV.

CONCLUSION

Both Ito and IKl are important K+ channel currents in immature human atrial myocytes. 4-AP can affect the inactivation and recovery of Ito at low concentration (0.3 mmol.L-1) and affect its activation at high concentration (1 mmol.L-1).

Authors+Show Affiliations

Department of Pharmacology, Institute of Materia Medica, Beijing 100050, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11270965

Citation

Liang, Y, et al. "Properties of Transient Outward Potassium Current and Inward Rectifier Potassium Current in Immature Human Atrial Myocytes." Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, vol. 20, no. 11, 1999, pp. 1005-10.
Liang Y, Sun XM, Wang XL. Properties of transient outward potassium current and inward rectifier potassium current in immature human atrial myocytes. Zhongguo Yao Li Xue Bao. 1999;20(11):1005-10.
Liang, Y., Sun, X. M., & Wang, X. L. (1999). Properties of transient outward potassium current and inward rectifier potassium current in immature human atrial myocytes. Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, 20(11), 1005-10.
Liang Y, Sun XM, Wang XL. Properties of Transient Outward Potassium Current and Inward Rectifier Potassium Current in Immature Human Atrial Myocytes. Zhongguo Yao Li Xue Bao. 1999;20(11):1005-10. PubMed PMID: 11270965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties of transient outward potassium current and inward rectifier potassium current in immature human atrial myocytes. AU - Liang,Y, AU - Sun,X M, AU - Wang,X L, PY - 2001/3/29/pubmed PY - 2001/4/3/medline PY - 2001/3/29/entrez SP - 1005 EP - 10 JF - Zhongguo yao li xue bao = Acta pharmacologica Sinica JO - Zhongguo Yao Li Xue Bao VL - 20 IS - 11 N2 - AIM: To study the properties of transient outward K+ current (Ito) and inward rectifier K+ current (IKl) in immature human heart. METHODS: Ito and IKl were recorded using whole-cell patch-clamp technique in atrial myocytes isolated from 12 immature (aged from 6 months to 5 a) human hearts. RESULTS: Ito was voltage-dependent, activated and inactivated rapidly. The IC50 (95% confidence limits) of 4-AP on Ito was 0.64 (0.48-0.87) mmol.L-1. 4-AP 1 mmol.L-1 shifted V1/2 of activation from (6.6 +/- 2.0) mV to (19.8 +/- 3.0) mV (n = 4-10, P < 0.01). 4-AP 0.3 mmol.L-1 changed V1/2 of inactivation from (-49 +/- 4) mV to (-61.4 +/- 2.1) mV (n = 3, P < 0.01), but there were no obvious influence on voltage-dependent activation of Ito (P > 0.05). At the same concentration, the recovery time constant (tau value) was prolonged from (108 +/- 16) ms to (220 +/- 67) ms (n = 3-12, P < 0.01). IKl was also voltage-dependent. Its reverse potential was -40 mV. CONCLUSION: Both Ito and IKl are important K+ channel currents in immature human atrial myocytes. 4-AP can affect the inactivation and recovery of Ito at low concentration (0.3 mmol.L-1) and affect its activation at high concentration (1 mmol.L-1). SN - 0253-9756 UR - https://www.unboundmedicine.com/medline/citation/11270965/Properties_of_transient_outward_potassium_current_and_inward_rectifier_potassium_current_in_immature_human_atrial_myocytes_ L2 - http://www.chinaphar.com/1671-4083/20/1005.pdf DB - PRIME DP - Unbound Medicine ER -