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Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad.
Fungal Genet Biol. 2000 Dec; 31(3):219-32.FG

Abstract

A full-length genomic clone encoding a class III chitin synthase (CHS) and one DNA fragment corresponding to a class IV CHS were isolated from the mycorrhizal fungus Tuber borchii and used for an extensive expression analysis, together with a previously identified DNA fragment corresponding to a class II CHS. All three Chs mRNAs are constitutively expressed in vegetative mycelia, regardless of the age, mode of growth, and proliferation capacity of the hyphae. A strikingly different situation was observed in ascomata, where class III and IV, but not class II, mRNAs are differentially expressed in a maturation stage-dependent manner and accumulate, respectively, in sporogenic and vegetative hyphae. These data, the first on the expression of distinct Chs mRNAs during fruitbody development, point to the different cellular roles that can be played by distinct chitin synthases in the differentiation of spores of sexual origin (CHS III) or in ascoma enlargement promoted by the growth of vegetative hyphae (CHS IV).

Authors+Show Affiliations

Centro di Studio sulla Micologia del Terreno, CNR, University of Torino, V.le Mattioli 25, 10125 Torino, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11273683

Citation

Balestrini, R, et al. "Differential Expression of Chitin Synthase III and IV mRNAs in Ascomata of Tuber Borchii Vittad." Fungal Genetics and Biology : FG & B, vol. 31, no. 3, 2000, pp. 219-32.
Balestrini R, Mainieri D, Soragni E, et al. Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad. Fungal Genet Biol. 2000;31(3):219-32.
Balestrini, R., Mainieri, D., Soragni, E., Garnero, L., Rollino, S., Viotti, A., Ottonello, S., & Bonfante, P. (2000). Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad. Fungal Genetics and Biology : FG & B, 31(3), 219-32.
Balestrini R, et al. Differential Expression of Chitin Synthase III and IV mRNAs in Ascomata of Tuber Borchii Vittad. Fungal Genet Biol. 2000;31(3):219-32. PubMed PMID: 11273683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad. AU - Balestrini,R, AU - Mainieri,D, AU - Soragni,E, AU - Garnero,L, AU - Rollino,S, AU - Viotti,A, AU - Ottonello,S, AU - Bonfante,P, PY - 2001/3/29/pubmed PY - 2001/6/2/medline PY - 2001/3/29/entrez SP - 219 EP - 32 JF - Fungal genetics and biology : FG & B JO - Fungal Genet. Biol. VL - 31 IS - 3 N2 - A full-length genomic clone encoding a class III chitin synthase (CHS) and one DNA fragment corresponding to a class IV CHS were isolated from the mycorrhizal fungus Tuber borchii and used for an extensive expression analysis, together with a previously identified DNA fragment corresponding to a class II CHS. All three Chs mRNAs are constitutively expressed in vegetative mycelia, regardless of the age, mode of growth, and proliferation capacity of the hyphae. A strikingly different situation was observed in ascomata, where class III and IV, but not class II, mRNAs are differentially expressed in a maturation stage-dependent manner and accumulate, respectively, in sporogenic and vegetative hyphae. These data, the first on the expression of distinct Chs mRNAs during fruitbody development, point to the different cellular roles that can be played by distinct chitin synthases in the differentiation of spores of sexual origin (CHS III) or in ascoma enlargement promoted by the growth of vegetative hyphae (CHS IV). SN - 1087-1845 UR - https://www.unboundmedicine.com/medline/citation/11273683/Differential_expression_of_chitin_synthase_III_and_IV_mRNAs_in_ascomata_of_Tuber_borchii_Vittad_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(00)91242-8 DB - PRIME DP - Unbound Medicine ER -