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Early detection of pancreatic cancer in patients with chronic pancreatitis: diagnostic utility of a K-ras point mutation in the pancreatic juice.
Am J Gastroenterol. 2001 Mar; 96(3):700-4.AJ

Abstract

OBJECTIVE

Point mutations of the K-ras oncogene at codon 12 have been described several months before the onset of pancreatic cancer in isolated cases of chronic pancreatitis (CP). The aim of this study was to evaluate the interest of a prospective follow-up of patients with CP and K-ras mutations at codon 12 in the detection of early pancreatic cancer.

METHODS

From February 1996 to March 1998, 36 patients (mean age 52.6 yr, 31 men, five women) with CP (alcoholic: 61.1%, pancreas divisum: 5.6%, autoimmune: 5.6%, unknown origin: 27.7%) were included and then prospectively monitored (median duration of 22 months) for detection of pancreatic carcinoma. K-ras point mutations were examined by two-step polymerase chain reaction combined with restriction enzyme digestion in pancreatic juice collected during endoscopic retrograde pancreatography.

RESULTS

Ten patients (27.8%) were positive for K-ras mutation. Patients with and without the mutation were not different with respect to age and sex ratio. K-ras mutations were homogeneously distributed according to the etiology (alcoholic vs nonalcoholic) and morphological characteristics (ductal stricture or mass vs none) of CP. A pancreatic carcinoma was discovered at an invasive stage in two patients, respectively at 7 and 17 months after disclosure of a K-ras mutation, versus none in patients without the mutation (p < 0.02).

CONCLUSIONS

Presence of a K-ras gene mutation is not rare in patients with CP and represents an increased risk of developing pancreatic cancer. However, its utility for the detection of early pancreatic cancer remains doubtful in clinical practice.

Authors+Show Affiliations

Services de Gastroentérologie, d'Oncologie et d'Endocrinologie Moléculaires, de Chirurgie Viscérale et Vasculaire, CHU Besançon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11280537

Citation

Queneau, P E., et al. "Early Detection of Pancreatic Cancer in Patients With Chronic Pancreatitis: Diagnostic Utility of a K-ras Point Mutation in the Pancreatic Juice." The American Journal of Gastroenterology, vol. 96, no. 3, 2001, pp. 700-4.
Queneau PE, Adessi GL, Thibault P, et al. Early detection of pancreatic cancer in patients with chronic pancreatitis: diagnostic utility of a K-ras point mutation in the pancreatic juice. Am J Gastroenterol. 2001;96(3):700-4.
Queneau, P. E., Adessi, G. L., Thibault, P., Cléau, D., Heyd, B., Mantion, G., & Carayon, P. (2001). Early detection of pancreatic cancer in patients with chronic pancreatitis: diagnostic utility of a K-ras point mutation in the pancreatic juice. The American Journal of Gastroenterology, 96(3), 700-4.
Queneau PE, et al. Early Detection of Pancreatic Cancer in Patients With Chronic Pancreatitis: Diagnostic Utility of a K-ras Point Mutation in the Pancreatic Juice. Am J Gastroenterol. 2001;96(3):700-4. PubMed PMID: 11280537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early detection of pancreatic cancer in patients with chronic pancreatitis: diagnostic utility of a K-ras point mutation in the pancreatic juice. AU - Queneau,P E, AU - Adessi,G L, AU - Thibault,P, AU - Cléau,D, AU - Heyd,B, AU - Mantion,G, AU - Carayon,P, PY - 2001/3/31/pubmed PY - 2001/5/1/medline PY - 2001/3/31/entrez SP - 700 EP - 4 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 96 IS - 3 N2 - OBJECTIVE: Point mutations of the K-ras oncogene at codon 12 have been described several months before the onset of pancreatic cancer in isolated cases of chronic pancreatitis (CP). The aim of this study was to evaluate the interest of a prospective follow-up of patients with CP and K-ras mutations at codon 12 in the detection of early pancreatic cancer. METHODS: From February 1996 to March 1998, 36 patients (mean age 52.6 yr, 31 men, five women) with CP (alcoholic: 61.1%, pancreas divisum: 5.6%, autoimmune: 5.6%, unknown origin: 27.7%) were included and then prospectively monitored (median duration of 22 months) for detection of pancreatic carcinoma. K-ras point mutations were examined by two-step polymerase chain reaction combined with restriction enzyme digestion in pancreatic juice collected during endoscopic retrograde pancreatography. RESULTS: Ten patients (27.8%) were positive for K-ras mutation. Patients with and without the mutation were not different with respect to age and sex ratio. K-ras mutations were homogeneously distributed according to the etiology (alcoholic vs nonalcoholic) and morphological characteristics (ductal stricture or mass vs none) of CP. A pancreatic carcinoma was discovered at an invasive stage in two patients, respectively at 7 and 17 months after disclosure of a K-ras mutation, versus none in patients without the mutation (p < 0.02). CONCLUSIONS: Presence of a K-ras gene mutation is not rare in patients with CP and represents an increased risk of developing pancreatic cancer. However, its utility for the detection of early pancreatic cancer remains doubtful in clinical practice. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/11280537/Early_detection_of_pancreatic_cancer_in_patients_with_chronic_pancreatitis:_diagnostic_utility_of_a_K_ras_point_mutation_in_the_pancreatic_juice_ L2 - http://Insights.ovid.com/pubmed?pmid=11280537 DB - PRIME DP - Unbound Medicine ER -