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Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments.
Appl Environ Microbiol. 2001 Apr; 67(4):1922-34.AE

Abstract

The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant (13)C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. (13)C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta-proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong (13)C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant (13)C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.

Authors+Show Affiliations

Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd., P.O. Box 628, Moss Landing, California 95039. orphan@mbari.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11282650

Citation

Orphan, V J., et al. "Comparative Analysis of Methane-oxidizing Archaea and Sulfate-reducing Bacteria in Anoxic Marine Sediments." Applied and Environmental Microbiology, vol. 67, no. 4, 2001, pp. 1922-34.
Orphan VJ, Hinrichs KU, Ussler W, et al. Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments. Appl Environ Microbiol. 2001;67(4):1922-34.
Orphan, V. J., Hinrichs, K. U., Ussler, W., Paull, C. K., Taylor, L. T., Sylva, S. P., Hayes, J. M., & Delong, E. F. (2001). Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments. Applied and Environmental Microbiology, 67(4), 1922-34.
Orphan VJ, et al. Comparative Analysis of Methane-oxidizing Archaea and Sulfate-reducing Bacteria in Anoxic Marine Sediments. Appl Environ Microbiol. 2001;67(4):1922-34. PubMed PMID: 11282650.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments. AU - Orphan,V J, AU - Hinrichs,K U, AU - Ussler,W,3rd AU - Paull,C K, AU - Taylor,L T, AU - Sylva,S P, AU - Hayes,J M, AU - Delong,E F, PY - 2001/4/3/pubmed PY - 2001/8/29/medline PY - 2001/4/3/entrez SP - 1922 EP - 34 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 67 IS - 4 N2 - The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant (13)C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. (13)C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta-proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong (13)C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant (13)C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/11282650/Comparative_analysis_of_methane_oxidizing_archaea_and_sulfate_reducing_bacteria_in_anoxic_marine_sediments_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=11282650 DB - PRIME DP - Unbound Medicine ER -