Tags

Type your tag names separated by a space and hit enter

Pyruvated galactose and oligosaccharides from Erwinia chrysanthemi Ech6 extracellular polysaccharide.
Carbohydr Res. 2001 Mar 09; 331(1):59-67.CR

Abstract

The acidic extracellular polysaccharide of Ech6 was depolymerized by fuming HCl. The pyruvated sugars were isolated and characterized by methods that included a combination of low-pressure gel-filtration and high-pH anion-exchange chromatographies, methylation linkage analyses, mass (GC-MS and MALDI-TOF MS) and 1H NMR (1D and 2D) spectroscopies. The following pyruvated sugars were obtained: 4,6-O-(1-carboxyethylidene)-D-Galp; 4,6-O-(1-carboxyethylidene)- alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3)-D-Galp; 4,6-O-(1-carboxyethylidene)-alpha-D-Galp-(1-->4)-alpha-D-GlcAp- (1-->3)-alpha-D-Galp-(1-->3)-L-Fucp; 4,6-O-(1-carboxyethylidene)-alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3) -alpha-D-Galp-(1-->3)-L-[beta-D-Glcp-(1-->4)]-Fucp. These oligosaccharides present potential haptenes for the development of specific antibodies and confirm the partial structure proposed previously for the extracellular polysaccharide from Erwinia chrysanthemi Ech6 [Yang, B. Y.; Gray, J. S. S.; Montgomery, R. Int. J. Biol. Macromol., 1994, 16, 306-312].

Authors+Show Affiliations

Yang BY
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA.
Brand J
No affiliation info available
Montgomery R
No affiliation info available

MeSH

Carbohydrate ConformationCarbohydrate SequenceDickeya chrysanthemiDisaccharidesGalactoseGas Chromatography-Mass SpectrometryHydrolysisMolecular Sequence DataNuclear Magnetic Resonance, BiomolecularOligosaccharidesPolysaccharides, BacterialPyruvatesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTrisaccharides

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11284505

Citation

Yang, B Y., et al. "Pyruvated Galactose and Oligosaccharides From Erwinia Chrysanthemi Ech6 Extracellular Polysaccharide." Carbohydrate Research, vol. 331, no. 1, 2001, pp. 59-67.
Yang BY, Brand J, Montgomery R. Pyruvated galactose and oligosaccharides from Erwinia chrysanthemi Ech6 extracellular polysaccharide. Carbohydr Res. 2001;331(1):59-67.
Yang, B. Y., Brand, J., & Montgomery, R. (2001). Pyruvated galactose and oligosaccharides from Erwinia chrysanthemi Ech6 extracellular polysaccharide. Carbohydrate Research, 331(1), 59-67.
Yang BY, Brand J, Montgomery R. Pyruvated Galactose and Oligosaccharides From Erwinia Chrysanthemi Ech6 Extracellular Polysaccharide. Carbohydr Res. 2001 Mar 9;331(1):59-67. PubMed PMID: 11284505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pyruvated galactose and oligosaccharides from Erwinia chrysanthemi Ech6 extracellular polysaccharide. AU - Yang,B Y, AU - Brand,J, AU - Montgomery,R, PY - 2001/4/4/pubmed PY - 2001/7/31/medline PY - 2001/4/4/entrez SP - 59 EP - 67 JF - Carbohydrate research JO - Carbohydr Res VL - 331 IS - 1 N2 - The acidic extracellular polysaccharide of Ech6 was depolymerized by fuming HCl. The pyruvated sugars were isolated and characterized by methods that included a combination of low-pressure gel-filtration and high-pH anion-exchange chromatographies, methylation linkage analyses, mass (GC-MS and MALDI-TOF MS) and 1H NMR (1D and 2D) spectroscopies. The following pyruvated sugars were obtained: 4,6-O-(1-carboxyethylidene)-D-Galp; 4,6-O-(1-carboxyethylidene)- alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3)-D-Galp; 4,6-O-(1-carboxyethylidene)-alpha-D-Galp-(1-->4)-alpha-D-GlcAp- (1-->3)-alpha-D-Galp-(1-->3)-L-Fucp; 4,6-O-(1-carboxyethylidene)-alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3) -alpha-D-Galp-(1-->3)-L-[beta-D-Glcp-(1-->4)]-Fucp. These oligosaccharides present potential haptenes for the development of specific antibodies and confirm the partial structure proposed previously for the extracellular polysaccharide from Erwinia chrysanthemi Ech6 [Yang, B. Y.; Gray, J. S. S.; Montgomery, R. Int. J. Biol. Macromol., 1994, 16, 306-312]. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/11284505/Pyruvated_galactose_and_oligosaccharides_from_Erwinia_chrysanthemi_Ech6_extracellular_polysaccharide_ DB - PRIME DP - Unbound Medicine ER -