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Microbiological and biochemical properties of canestrato pugliese hard cheese supplemented with bifidobacteria.
J Dairy Sci. 2001 Mar; 84(3):551-61.JD

Abstract

Canestrato Pugliese cheeses from ewe milk were produced according to a traditional protocol and by adding 7.0 log10 cfu of fresh cells per gram of Bifidobacterium bifidum Bb02, Bifidobacterium longum Bb46, or both species. The traditional technology was modified slightly to favor the survival of probiotic microorganisms. After 56 d of ripening, the survival of B. bifidum Bb02 and B. longum Bb46 was 6.0 and 5.0 log10 cfu/g, respectively. After 19 d cheeses contained ca. 7.0 log10 cfu/g of bifidobacteria. Compared to traditional cheese, the addition of bifidobacteria seemed to support the growth and survival of mesophilic lactobacilli and Streptococcus thermophilus, used as starter, during ripening. No significant differences were observed in the main chemical composition, and only a slightly higher concentration of acetic acid was found in cheeses with bifidobacteria added. On the contrary, alpha- and beta-galactosidase activities were markedly more pronounced in the presence of bifidobacteria, especially with B. bifidum Bb02. In contrast with traditional cheese, the lactose was completely hydrolyzed in cheeses made with bifidobacteria. Urea-PAGE electrophoresis of the pH 4.6-soluble and pH 4.6-insoluble N fractions did not show appreciable variations. Only the reversed-phase-HPLC analysis of the pH 4.6-soluble N showed a slightly more complex profile in the presence of bifidobacteria. This finding was in agreement with the higher value of the pH 4.6-soluble N/total N ratio and with the more pronounced amino-, imino-, and dipeptidase activities found in all the cheeses with the bifidobacteria added, especially B. bifidum Bb02. No differences were found in the free amino acid and free fatty acid contents. The amino acids glutamic acid, valine, proline, leucine, and lysine and the fatty acids butyric, caproic, capric, and oleic acids were found at the highest concentrations. The sensory evaluation did not show significant differences, and Canestrato Pugliese cheeses were characterized by small and uniformly distributed eyes, were pale yellow, had an elastic consistency and a Pecorino-like smell, were very salty, and tended to be moderately piquant.

Authors+Show Affiliations

Corbo MR
Istituto di Produzioni e Preparazioni Alimentari, Facoltà di Agraria, Università degli Studi di Foggia, Italy.
Albenzio M
No affiliation info available
De Angelis M
No affiliation info available
Sevi A
No affiliation info available
Gobbetti M
No affiliation info available

MeSH

Amino AcidsAnimalsBifidobacteriumCheeseChromatography, High Pressure LiquidColony Count, MicrobialFatty AcidsGalactosidasesHydrogen-Ion ConcentrationHydrolysisNitrogenProbioticsSheepStreptococcusTaste

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11286406

Citation

Corbo, M R., et al. "Microbiological and Biochemical Properties of Canestrato Pugliese Hard Cheese Supplemented With Bifidobacteria." Journal of Dairy Science, vol. 84, no. 3, 2001, pp. 551-61.
Corbo MR, Albenzio M, De Angelis M, et al. Microbiological and biochemical properties of canestrato pugliese hard cheese supplemented with bifidobacteria. J Dairy Sci. 2001;84(3):551-61.
Corbo, M. R., Albenzio, M., De Angelis, M., Sevi, A., & Gobbetti, M. (2001). Microbiological and biochemical properties of canestrato pugliese hard cheese supplemented with bifidobacteria. Journal of Dairy Science, 84(3), 551-61.
Corbo MR, et al. Microbiological and Biochemical Properties of Canestrato Pugliese Hard Cheese Supplemented With Bifidobacteria. J Dairy Sci. 2001;84(3):551-61. PubMed PMID: 11286406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbiological and biochemical properties of canestrato pugliese hard cheese supplemented with bifidobacteria. AU - Corbo,M R, AU - Albenzio,M, AU - De Angelis,M, AU - Sevi,A, AU - Gobbetti,M, PY - 2001/4/5/pubmed PY - 2001/10/23/medline PY - 2001/4/5/entrez SP - 551 EP - 61 JF - Journal of dairy science JO - J Dairy Sci VL - 84 IS - 3 N2 - Canestrato Pugliese cheeses from ewe milk were produced according to a traditional protocol and by adding 7.0 log10 cfu of fresh cells per gram of Bifidobacterium bifidum Bb02, Bifidobacterium longum Bb46, or both species. The traditional technology was modified slightly to favor the survival of probiotic microorganisms. After 56 d of ripening, the survival of B. bifidum Bb02 and B. longum Bb46 was 6.0 and 5.0 log10 cfu/g, respectively. After 19 d cheeses contained ca. 7.0 log10 cfu/g of bifidobacteria. Compared to traditional cheese, the addition of bifidobacteria seemed to support the growth and survival of mesophilic lactobacilli and Streptococcus thermophilus, used as starter, during ripening. No significant differences were observed in the main chemical composition, and only a slightly higher concentration of acetic acid was found in cheeses with bifidobacteria added. On the contrary, alpha- and beta-galactosidase activities were markedly more pronounced in the presence of bifidobacteria, especially with B. bifidum Bb02. In contrast with traditional cheese, the lactose was completely hydrolyzed in cheeses made with bifidobacteria. Urea-PAGE electrophoresis of the pH 4.6-soluble and pH 4.6-insoluble N fractions did not show appreciable variations. Only the reversed-phase-HPLC analysis of the pH 4.6-soluble N showed a slightly more complex profile in the presence of bifidobacteria. This finding was in agreement with the higher value of the pH 4.6-soluble N/total N ratio and with the more pronounced amino-, imino-, and dipeptidase activities found in all the cheeses with the bifidobacteria added, especially B. bifidum Bb02. No differences were found in the free amino acid and free fatty acid contents. The amino acids glutamic acid, valine, proline, leucine, and lysine and the fatty acids butyric, caproic, capric, and oleic acids were found at the highest concentrations. The sensory evaluation did not show significant differences, and Canestrato Pugliese cheeses were characterized by small and uniformly distributed eyes, were pale yellow, had an elastic consistency and a Pecorino-like smell, were very salty, and tended to be moderately piquant. SN - 0022-0302 UR - https://www.unboundmedicine.com/medline/citation/11286406/Microbiological_and_biochemical_properties_of_canestrato_pugliese_hard_cheese_supplemented_with_bifidobacteria_ DB - PRIME DP - Unbound Medicine ER -