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Developmental expression of vascular endothelial growth factor (VEGF) receptors and VEGF binding in ovine placenta and fetal membranes.
Placenta. 2001 Apr; 22(4):265-75.P

Abstract

The receptor tyrosine kinases, kinase-insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), and their ligand vascular endothelial growth factor (VEGF) are essential for the development and maintenance of placental vascular function during pregnancy. To further understand the role of VEGF in mediating angiogenesis and vascular permeability during development, the cellular localization of KDR and Flt-1 mRNA and protein, and the distribution of(125)I-VEGF binding sites in placenta, chorion and amnion of ovine fetuses were examined at three different gestational ages. In placentae at 62, 103 and 142 days, the predominant site of KDR mRNA and protein, and VEGF binding was the maternal vascular endothelium. In addition, a specific, although weak, signal for KDR mRNA was found in the maternal epithelium. At 103 and 142 days but not 62 days gestation, KDR mRNA and protein as well as VEGF binding sites were abundantly present in the endothelium of villous blood vessels. In the fetal membranes at 62, 103 and 142 days gestation, KDR mRNA and protein were expressed in the amniotic epithelium and intramembranous blood vessel endothelium, where binding of(125)I-VEGF was strong. There was no KDR mRNA or VEGF binding in the chorionic cytotrophoblast. Flt-1 expression was not detectable in placentae or fetal membranes at the three ages studied. In summary, the results demonstrated that VEGF receptors are present in the maternal and fetal vasculatures of the ovine placenta. This expression is consistent with a capillary growth-promoting function of KDR and its ligand VEGF. Further, the presence of KDR and VEGF binding sites in ovine fetal membranes suggests a role for VEGF in promoting intramembranous vascularity and permeability throughout gestation.

Authors+Show Affiliations

Division of Perinatal Medicine, Department of Reproductive Medicine, University of California, San Diego, La Jolla, California 92093-0802, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11286562

Citation

Bogic, L V., et al. "Developmental Expression of Vascular Endothelial Growth Factor (VEGF) Receptors and VEGF Binding in Ovine Placenta and Fetal Membranes." Placenta, vol. 22, no. 4, 2001, pp. 265-75.
Bogic LV, Brace RA, Cheung CY. Developmental expression of vascular endothelial growth factor (VEGF) receptors and VEGF binding in ovine placenta and fetal membranes. Placenta. 2001;22(4):265-75.
Bogic, L. V., Brace, R. A., & Cheung, C. Y. (2001). Developmental expression of vascular endothelial growth factor (VEGF) receptors and VEGF binding in ovine placenta and fetal membranes. Placenta, 22(4), 265-75.
Bogic LV, Brace RA, Cheung CY. Developmental Expression of Vascular Endothelial Growth Factor (VEGF) Receptors and VEGF Binding in Ovine Placenta and Fetal Membranes. Placenta. 2001;22(4):265-75. PubMed PMID: 11286562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental expression of vascular endothelial growth factor (VEGF) receptors and VEGF binding in ovine placenta and fetal membranes. AU - Bogic,L V, AU - Brace,R A, AU - Cheung,C Y, PY - 2001/4/5/pubmed PY - 2001/7/6/medline PY - 2001/4/5/entrez SP - 265 EP - 75 JF - Placenta JO - Placenta VL - 22 IS - 4 N2 - The receptor tyrosine kinases, kinase-insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), and their ligand vascular endothelial growth factor (VEGF) are essential for the development and maintenance of placental vascular function during pregnancy. To further understand the role of VEGF in mediating angiogenesis and vascular permeability during development, the cellular localization of KDR and Flt-1 mRNA and protein, and the distribution of(125)I-VEGF binding sites in placenta, chorion and amnion of ovine fetuses were examined at three different gestational ages. In placentae at 62, 103 and 142 days, the predominant site of KDR mRNA and protein, and VEGF binding was the maternal vascular endothelium. In addition, a specific, although weak, signal for KDR mRNA was found in the maternal epithelium. At 103 and 142 days but not 62 days gestation, KDR mRNA and protein as well as VEGF binding sites were abundantly present in the endothelium of villous blood vessels. In the fetal membranes at 62, 103 and 142 days gestation, KDR mRNA and protein were expressed in the amniotic epithelium and intramembranous blood vessel endothelium, where binding of(125)I-VEGF was strong. There was no KDR mRNA or VEGF binding in the chorionic cytotrophoblast. Flt-1 expression was not detectable in placentae or fetal membranes at the three ages studied. In summary, the results demonstrated that VEGF receptors are present in the maternal and fetal vasculatures of the ovine placenta. This expression is consistent with a capillary growth-promoting function of KDR and its ligand VEGF. Further, the presence of KDR and VEGF binding sites in ovine fetal membranes suggests a role for VEGF in promoting intramembranous vascularity and permeability throughout gestation. SN - 0143-4004 UR - https://www.unboundmedicine.com/medline/citation/11286562/Developmental_expression_of_vascular_endothelial_growth_factor__VEGF__receptors_and_VEGF_binding_in_ovine_placenta_and_fetal_membranes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(01)90627-7 DB - PRIME DP - Unbound Medicine ER -