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Introduction of a rapid dipstick assay for the detection of Leptospira-specific immunoglobulin m antibodies in the laboratory diagnosis of leptospirosis in a hospital in Makassar, Indonesia.
Southeast Asian J Trop Med Public Health. 2000 Sep; 31(3):515-20.SA

Abstract

An easy, rapid and robust dipstick assay for detection of leptospira-specific immunoglobulin M (IgM) antibodies was evaluated on 403 patients admitted for hospitalization because of fever. The clinical symptoms and signs of 35 patients were consistent with leptospirosis. The final diagnosis for the remaining patients was as follows: 136 with typhoid fever, 82 with hepatitis, 74 with malaria, 48 with infections of the respiratory tract, and 20 with fever of unknown origin. The clinical diagnosis of leptospirosis was confirmed for 24 (68.6%) patients by the combined results of the microscopic agglutination test (MAT), the reference test for leptospirosis, and of IgM ELISA, a standard laboratory test for the serodiagnosis of leptospirosis. In addition, serum specimens from 8 (2.2%) patients with a final clinical diagnosis other than leptospirosis were found to be positive in MAT and/or IgM ELISA. Compared with the results of MAT and IgM ELISA a sensitivity of 91.6% and specificity of 93.6% was calculated for the dipstick assay. Most of the serum samples from the laboratory confirmed patients gave a moderate to strong staining intensity of the antigen band of the dipstick and were easy to read. The results demonstrate that the dipstick assay is convenient to use and allows the rapid and accurate confirmation of patients with clinical suspicion of leptospirosis in areas where the disease is endemic.

Authors+Show Affiliations

Department of Medical Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. hattaram@indosat.net.idNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11289012

Citation

Hatta, M, et al. "Introduction of a Rapid Dipstick Assay for the Detection of Leptospira-specific Immunoglobulin M Antibodies in the Laboratory Diagnosis of Leptospirosis in a Hospital in Makassar, Indonesia." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 31, no. 3, 2000, pp. 515-20.
Hatta M, Smits HL, Gussenhoven GC, et al. Introduction of a rapid dipstick assay for the detection of Leptospira-specific immunoglobulin m antibodies in the laboratory diagnosis of leptospirosis in a hospital in Makassar, Indonesia. Southeast Asian J Trop Med Public Health. 2000;31(3):515-20.
Hatta, M., Smits, H. L., Gussenhoven, G. C., & Gooskens, J. (2000). Introduction of a rapid dipstick assay for the detection of Leptospira-specific immunoglobulin m antibodies in the laboratory diagnosis of leptospirosis in a hospital in Makassar, Indonesia. The Southeast Asian Journal of Tropical Medicine and Public Health, 31(3), 515-20.
Hatta M, et al. Introduction of a Rapid Dipstick Assay for the Detection of Leptospira-specific Immunoglobulin M Antibodies in the Laboratory Diagnosis of Leptospirosis in a Hospital in Makassar, Indonesia. Southeast Asian J Trop Med Public Health. 2000;31(3):515-20. PubMed PMID: 11289012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Introduction of a rapid dipstick assay for the detection of Leptospira-specific immunoglobulin m antibodies in the laboratory diagnosis of leptospirosis in a hospital in Makassar, Indonesia. AU - Hatta,M, AU - Smits,H L, AU - Gussenhoven,G C, AU - Gooskens,J, PY - 2001/4/6/pubmed PY - 2001/7/19/medline PY - 2001/4/6/entrez SP - 515 EP - 20 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 31 IS - 3 N2 - An easy, rapid and robust dipstick assay for detection of leptospira-specific immunoglobulin M (IgM) antibodies was evaluated on 403 patients admitted for hospitalization because of fever. The clinical symptoms and signs of 35 patients were consistent with leptospirosis. The final diagnosis for the remaining patients was as follows: 136 with typhoid fever, 82 with hepatitis, 74 with malaria, 48 with infections of the respiratory tract, and 20 with fever of unknown origin. The clinical diagnosis of leptospirosis was confirmed for 24 (68.6%) patients by the combined results of the microscopic agglutination test (MAT), the reference test for leptospirosis, and of IgM ELISA, a standard laboratory test for the serodiagnosis of leptospirosis. In addition, serum specimens from 8 (2.2%) patients with a final clinical diagnosis other than leptospirosis were found to be positive in MAT and/or IgM ELISA. Compared with the results of MAT and IgM ELISA a sensitivity of 91.6% and specificity of 93.6% was calculated for the dipstick assay. Most of the serum samples from the laboratory confirmed patients gave a moderate to strong staining intensity of the antigen band of the dipstick and were easy to read. The results demonstrate that the dipstick assay is convenient to use and allows the rapid and accurate confirmation of patients with clinical suspicion of leptospirosis in areas where the disease is endemic. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/11289012/Introduction_of_a_rapid_dipstick_assay_for_the_detection_of_Leptospira_specific_immunoglobulin_m_antibodies_in_the_laboratory_diagnosis_of_leptospirosis_in_a_hospital_in_Makassar_Indonesia_ L2 - https://www.diseaseinfosearch.org/result/4178 DB - PRIME DP - Unbound Medicine ER -