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Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro.
Phytomedicine. 2001 Jan; 8(1):53-8.P

Abstract

Sulfated polysaccharides exhibit many biological properties such as antiviral and anticoagulant activities. Herein, we report the antiviral activity of sulfated galactans extracted from the red sea-weed Bostrychia montagnei against herpes simplex virus types 1 (strain F and the thymidine kinase-deficient strains Field and B2006) and 2 (strain G). Two crude extracts obtained with cold and hot water as well as some fractions obtained by anion exchange chromatography, inhibited significantly the replication of the different strains of herpesviruses as determined by plaque reduction assays. The inhibitory effect of the compounds studied here took place only when they were added during the adsorption period. They were found to be highly selective antiviral substances, causing no impairment of Vero cell viability. Furthermore, they had no direct inactivating effect on virions by incubation in a virucidal assay. The antiviral activity could be correlated with the molecular weight and sulfate content of the polysaccharides. Although sulfated polysaccharides are generally endowed with anticoagulant properties, the results of the activated partial thromboplastin time and the thrombine time assays indicated that the natural sulfated polysaccharides from Bostrychia montagnei have very low anticoagulant activity, confirming that there is no relation between the antiviral and anticoagulant properties.

Authors+Show Affiliations

Duarte ME
Departamento de Bioquímica, Universidade Federal do Paraná, Centro Politécnico, Curitiba, Brasil.
Noseda DG
No affiliation info available
Noseda MD
No affiliation info available
Tulio S
No affiliation info available
Pujol CA
No affiliation info available
Damonte EB
No affiliation info available

MeSH

AnimalsAnticoagulantsAntiviral AgentsChlorocebus aethiopsGalactansPlant ExtractsPlants, MedicinalRhodophytaSimplexvirusVero Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11292240

Clinical Trial Links

(CBDRA60) to Prevent or Reduce Symptoms of COVID-19 and Prevention of Post-Acute Sequelae of SARS-CoV-2 Infection PASC

Citation

Duarte, M E., et al. "Inhibitory Effect of Sulfated Galactans From the Marine Alga Bostrychia Montagnei On Herpes Simplex Virus Replication in Vitro." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 8, no. 1, 2001, pp. 53-8.
Duarte ME, Noseda DG, Noseda MD, et al. Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro. Phytomedicine. 2001;8(1):53-8.
Duarte, M. E., Noseda, D. G., Noseda, M. D., Tulio, S., Pujol, C. A., & Damonte, E. B. (2001). Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 8(1), 53-8.
Duarte ME, et al. Inhibitory Effect of Sulfated Galactans From the Marine Alga Bostrychia Montagnei On Herpes Simplex Virus Replication in Vitro. Phytomedicine. 2001;8(1):53-8. PubMed PMID: 11292240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro. AU - Duarte,M E, AU - Noseda,D G, AU - Noseda,M D, AU - Tulio,S, AU - Pujol,C A, AU - Damonte,E B, PY - 2001/4/9/pubmed PY - 2001/7/13/medline PY - 2001/4/9/entrez SP - 53 EP - 8 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 8 IS - 1 N2 - Sulfated polysaccharides exhibit many biological properties such as antiviral and anticoagulant activities. Herein, we report the antiviral activity of sulfated galactans extracted from the red sea-weed Bostrychia montagnei against herpes simplex virus types 1 (strain F and the thymidine kinase-deficient strains Field and B2006) and 2 (strain G). Two crude extracts obtained with cold and hot water as well as some fractions obtained by anion exchange chromatography, inhibited significantly the replication of the different strains of herpesviruses as determined by plaque reduction assays. The inhibitory effect of the compounds studied here took place only when they were added during the adsorption period. They were found to be highly selective antiviral substances, causing no impairment of Vero cell viability. Furthermore, they had no direct inactivating effect on virions by incubation in a virucidal assay. The antiviral activity could be correlated with the molecular weight and sulfate content of the polysaccharides. Although sulfated polysaccharides are generally endowed with anticoagulant properties, the results of the activated partial thromboplastin time and the thrombine time assays indicated that the natural sulfated polysaccharides from Bostrychia montagnei have very low anticoagulant activity, confirming that there is no relation between the antiviral and anticoagulant properties. SN - 0944-7113 UR - https://www.unboundmedicine.com/medline/citation/11292240/Inhibitory_effect_of_sulfated_galactans_from_the_marine_alga_Bostrychia_montagnei_on_herpes_simplex_virus_replication_in_vitro_ DB - PRIME DP - Unbound Medicine ER -