Tags

Type your tag names separated by a space and hit enter

Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma.
Cancer Res. 2001 Apr 01; 61(7):3176-81.CR

Abstract

By using a cDNA array representing 14,000 cDNA clusters, we studied the expression profiles in paired clinical hepatocellular carcinoma (HCC) samples and the distal nontumorous liver tissues from the same patients. Despite the significant heterogeneity among the clinical samples, 72 genes (including 30 novel genes) were down-regulated and 84 genes (including 48 novel genes) were up-regulated in >50% of the cancer samples that were identified. The alterations in gene expression levels were confirmed by Northern blot and reverse-transcription PCR in all of 4 randomly selected genes. It was conspicuous that 21 of 38 hepatocarcinoma (HCC) down-regulated genes studied previously were reportedly regulated by a group of liver-enriched transcription factors (LETFs), and 12 of 36 HCC up-regulated genes studied previously were involved in protein translation. Reexamination of the cDNA array data further revealed that most of the genes known to be regulated by LETFs were down-regulated in at least a portion of the HCC samples. Among the LETFs, the expression level of CCAAT/enhancer-binding protein (C/EBP) alpha was down-regulated in cancer, whereas hepatocyte nuclear factor 1 (HNF-1), HNF-3beta, HNF-4alpha, and HNF-4gamma were up-regulated. The expression profiling thus suggested multiple regulatory pathways involved in HCC, especially that related to LETFs.

Authors+Show Affiliations

Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11306505

Citation

Xu, L, et al. "Expression Profiling Suggested a Regulatory Role of Liver-enriched Transcription Factors in Human Hepatocellular Carcinoma." Cancer Research, vol. 61, no. 7, 2001, pp. 3176-81.
Xu L, Hui L, Wang S, et al. Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma. Cancer Res. 2001;61(7):3176-81.
Xu, L., Hui, L., Wang, S., Gong, J., Jin, Y., Wang, Y., Ji, Y., Wu, X., Han, Z., & Hu, G. (2001). Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma. Cancer Research, 61(7), 3176-81.
Xu L, et al. Expression Profiling Suggested a Regulatory Role of Liver-enriched Transcription Factors in Human Hepatocellular Carcinoma. Cancer Res. 2001 Apr 1;61(7):3176-81. PubMed PMID: 11306505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma. AU - Xu,L, AU - Hui,L, AU - Wang,S, AU - Gong,J, AU - Jin,Y, AU - Wang,Y, AU - Ji,Y, AU - Wu,X, AU - Han,Z, AU - Hu,G, PY - 2001/4/18/pubmed PY - 2001/5/5/medline PY - 2001/4/18/entrez SP - 3176 EP - 81 JF - Cancer research JO - Cancer Res VL - 61 IS - 7 N2 - By using a cDNA array representing 14,000 cDNA clusters, we studied the expression profiles in paired clinical hepatocellular carcinoma (HCC) samples and the distal nontumorous liver tissues from the same patients. Despite the significant heterogeneity among the clinical samples, 72 genes (including 30 novel genes) were down-regulated and 84 genes (including 48 novel genes) were up-regulated in >50% of the cancer samples that were identified. The alterations in gene expression levels were confirmed by Northern blot and reverse-transcription PCR in all of 4 randomly selected genes. It was conspicuous that 21 of 38 hepatocarcinoma (HCC) down-regulated genes studied previously were reportedly regulated by a group of liver-enriched transcription factors (LETFs), and 12 of 36 HCC up-regulated genes studied previously were involved in protein translation. Reexamination of the cDNA array data further revealed that most of the genes known to be regulated by LETFs were down-regulated in at least a portion of the HCC samples. Among the LETFs, the expression level of CCAAT/enhancer-binding protein (C/EBP) alpha was down-regulated in cancer, whereas hepatocyte nuclear factor 1 (HNF-1), HNF-3beta, HNF-4alpha, and HNF-4gamma were up-regulated. The expression profiling thus suggested multiple regulatory pathways involved in HCC, especially that related to LETFs. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/11306505/Expression_profiling_suggested_a_regulatory_role_of_liver_enriched_transcription_factors_in_human_hepatocellular_carcinoma_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11306505 DB - PRIME DP - Unbound Medicine ER -