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Virgin olive oil phenolic compounds: binding to human low density lipoprotein (LDL) and effect on LDL oxidation.
Int J Clin Pharmacol Res. 2000; 20(3-4):49-54.IJ

Abstract

Oxidation of low density lipoproteins (LDL) appears to occur predominantly in arterial intima in microdomains sequestered from antioxidants of plasma. Therefore phenolic compounds which are able to bind LDL are good drug candidates for the effective prevention of lipid peroxidation and atherosclerotic processes. Plasma from healthy volunteers on nonsupplemented diets was incubated with virgin olive oil phenolic extracts (0-200 mg/l, caffeic acid equivalents). Phenolic compounds in LDL were measured by high-performance liquid chromatography-diode array detection (HPLC-DAD). Copper-mediated LDL oxidation was performed, and conjugated dienes formation was monitored. After plasma preincubation with olive oil phenolic compounds (OOPC), an increased OOPC-concentration dependent was observed in the total phenolic content of LDL (p < 0.001, ANOVA) as well as in the lag time before conjugated diene formation (p < 0.001, ANOVA). Rutin and four phenolics with flavonoid-like spectra were found to be bound to the LDL control. These phenolics, together with tyrosol which was not present in the LDL control, significantly increased in LDL (p < 0.05) after plasma incubation with OOPC. These results show the ability of tyrosol to bind LDL in vitro and the capacity of virgin olive oil phenolics to protect other phenolic compounds previously bound to LDL. These results provide further evidence that phenolic compounds bound to LDL are likely to protect LDL from oxidation.

Authors+Show Affiliations

Unitat de Lípids i Epidemiologia Cardiovascular, Institut Municipal d'Investigació Mèdica (IMIM), Carrer Doctor Aiguader, 80, 08003 Barcelona, Spain. mcovas@imim.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11314237

Citation

Covas, M I., et al. "Virgin Olive Oil Phenolic Compounds: Binding to Human Low Density Lipoprotein (LDL) and Effect On LDL Oxidation." International Journal of Clinical Pharmacology Research, vol. 20, no. 3-4, 2000, pp. 49-54.
Covas MI, Fitó M, Lamuela-Raventós RM, et al. Virgin olive oil phenolic compounds: binding to human low density lipoprotein (LDL) and effect on LDL oxidation. Int J Clin Pharmacol Res. 2000;20(3-4):49-54.
Covas, M. I., Fitó, M., Lamuela-Raventós, R. M., Sebastiá, N., de la Torre-Boronat, C., & Marrugat, J. (2000). Virgin olive oil phenolic compounds: binding to human low density lipoprotein (LDL) and effect on LDL oxidation. International Journal of Clinical Pharmacology Research, 20(3-4), 49-54.
Covas MI, et al. Virgin Olive Oil Phenolic Compounds: Binding to Human Low Density Lipoprotein (LDL) and Effect On LDL Oxidation. Int J Clin Pharmacol Res. 2000;20(3-4):49-54. PubMed PMID: 11314237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virgin olive oil phenolic compounds: binding to human low density lipoprotein (LDL) and effect on LDL oxidation. AU - Covas,M I, AU - Fitó,M, AU - Lamuela-Raventós,R M, AU - Sebastiá,N, AU - de la Torre-Boronat,C, AU - Marrugat,J, PY - 2001/4/21/pubmed PY - 2001/8/17/medline PY - 2001/4/21/entrez SP - 49 EP - 54 JF - International journal of clinical pharmacology research JO - Int J Clin Pharmacol Res VL - 20 IS - 3-4 N2 - Oxidation of low density lipoproteins (LDL) appears to occur predominantly in arterial intima in microdomains sequestered from antioxidants of plasma. Therefore phenolic compounds which are able to bind LDL are good drug candidates for the effective prevention of lipid peroxidation and atherosclerotic processes. Plasma from healthy volunteers on nonsupplemented diets was incubated with virgin olive oil phenolic extracts (0-200 mg/l, caffeic acid equivalents). Phenolic compounds in LDL were measured by high-performance liquid chromatography-diode array detection (HPLC-DAD). Copper-mediated LDL oxidation was performed, and conjugated dienes formation was monitored. After plasma preincubation with olive oil phenolic compounds (OOPC), an increased OOPC-concentration dependent was observed in the total phenolic content of LDL (p < 0.001, ANOVA) as well as in the lag time before conjugated diene formation (p < 0.001, ANOVA). Rutin and four phenolics with flavonoid-like spectra were found to be bound to the LDL control. These phenolics, together with tyrosol which was not present in the LDL control, significantly increased in LDL (p < 0.05) after plasma incubation with OOPC. These results show the ability of tyrosol to bind LDL in vitro and the capacity of virgin olive oil phenolics to protect other phenolic compounds previously bound to LDL. These results provide further evidence that phenolic compounds bound to LDL are likely to protect LDL from oxidation. SN - 0251-1649 UR - https://www.unboundmedicine.com/medline/citation/11314237/Virgin_olive_oil_phenolic_compounds:_binding_to_human_low_density_lipoprotein__LDL__and_effect_on_LDL_oxidation_ L2 - https://ClinicalTrials.gov/search/term=11314237 [PUBMED-IDS] DB - PRIME DP - Unbound Medicine ER -