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Method validation for diclofenac sodium in pharmaceuticals by capillary electrophoresis.
J Capill Electrophor Microchip Technol. 1999 May-Aug; 6(3-4):125-9.JC

Abstract

A capillary electrophoresis method for the determination of diclofenac sodium in a commercial and simulated tablet formation has been described. The aim of this research was to develop a rapid, simple, efficient, and economical method to determine diclofenac sodium in pharmaceutials. The analysis was carried out in a bare silica capillary (75 pm i.d.) with a total length of 50 cm (28 cm to the detector) with a buffer solution containing 20 mM sodium tetraborate, pH 9.23. The applied voltage was 20 kV. Detection was achieved by ultraviolet absorption at 276 nm. Acetaminophen was used as an internal standard. The calibration curve was linear to the concentration range from 40.0 to 120.0 microg/mL with a correlation coefficient of 0.9992. The percentage recovery was found to be 103.12 +/- 0.91. The results showed that the method allows the determination of diclofenac sodium in commercially available pharmaceutical preparations.

Authors+Show Affiliations

Prado MS
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, SP, Brazil.
Steppe M
No affiliation info available
Tavares MF
No affiliation info available
Kedor-Hackman ER
No affiliation info available
Santoro MI
No affiliation info available

MeSH

CalibrationDiclofenacElectrophoresis, CapillaryPharmaceutical PreparationsReproducibility of ResultsTablets

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11315152

Citation

Prado, M S., et al. "Method Validation for Diclofenac Sodium in Pharmaceuticals By Capillary Electrophoresis." Journal of Capillary Electrophoresis and Microchip Technology, vol. 6, no. 3-4, 1999, pp. 125-9.
Prado MS, Steppe M, Tavares MF, et al. Method validation for diclofenac sodium in pharmaceuticals by capillary electrophoresis. J Capill Electrophor Microchip Technol. 1999;6(3-4):125-9.
Prado, M. S., Steppe, M., Tavares, M. F., Kedor-Hackman, E. R., & Santoro, M. I. (1999). Method validation for diclofenac sodium in pharmaceuticals by capillary electrophoresis. Journal of Capillary Electrophoresis and Microchip Technology, 6(3-4), 125-9.
Prado MS, et al. Method Validation for Diclofenac Sodium in Pharmaceuticals By Capillary Electrophoresis. J Capill Electrophor Microchip Technol. 1999 May-Aug;6(3-4):125-9. PubMed PMID: 11315152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method validation for diclofenac sodium in pharmaceuticals by capillary electrophoresis. AU - Prado,M S, AU - Steppe,M, AU - Tavares,M F, AU - Kedor-Hackman,E R, AU - Santoro,M I, PY - 2001/4/21/pubmed PY - 2001/8/10/medline PY - 2001/4/21/entrez SP - 125 EP - 9 JF - Journal of capillary electrophoresis and microchip technology JO - J Capill Electrophor Microchip Technol VL - 6 IS - 3-4 N2 - A capillary electrophoresis method for the determination of diclofenac sodium in a commercial and simulated tablet formation has been described. The aim of this research was to develop a rapid, simple, efficient, and economical method to determine diclofenac sodium in pharmaceutials. The analysis was carried out in a bare silica capillary (75 pm i.d.) with a total length of 50 cm (28 cm to the detector) with a buffer solution containing 20 mM sodium tetraborate, pH 9.23. The applied voltage was 20 kV. Detection was achieved by ultraviolet absorption at 276 nm. Acetaminophen was used as an internal standard. The calibration curve was linear to the concentration range from 40.0 to 120.0 microg/mL with a correlation coefficient of 0.9992. The percentage recovery was found to be 103.12 +/- 0.91. The results showed that the method allows the determination of diclofenac sodium in commercially available pharmaceutical preparations. SN - 1946-4940 UR - https://www.unboundmedicine.com/medline/citation/11315152/Method_validation_for_diclofenac_sodium_in_pharmaceuticals_by_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -