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On-line solid-phase preconcentration for sensitivity enhancement in capillary electrophoresis.
J Capill Electrophor Microchip Technol. 1999 May-Aug; 6(3-4):61-73.JC

Abstract

Capillary electrophoresis is attractive for the analysis of biological samples because only a few nanoliters of sample need to be injected. Indeed, optimal resolution is achieved when the injected volume is 1% or less of the total capillary volume. Unfortunately, this advantage leads to severe detection limitations compounded by the fact that many analytes in a biological sample are present at very low concentrations. To overcome the detection sensitivity limitations of CE, nonspecific, on-line preconcentration has been employed in a variety of applications. This technique is based on inserting a small quantity of reversed-phase material (e.g., C18 particles or membrane) near the inlet of the CE capillary for sample enrichment by solid-phase extraction. A detachable cartridge containing the solid phase is relatively simple to construct and permits the injection of large sample volumes (1-100 microL) into the capillary. Elution of adsorbed analyte in less than 100 nL of solvent permits a 100-1000 fold improvement in the effective concentration limit of detection, depending on the hydrophobicity of the compound. Detection of analytes present in complex mixtures at concentrations as low as 20 amol/microL has been reported when using an on-line preconcentration device in conjunction with CE-mass spectrometry. In this review, the potential merits of the technique are described and some examples of direct analysis of biological samples without rigorous off-line pretreatment are given.

Authors+Show Affiliations

Bonneil E
Department of Chemistry, University of Montreal, Québec, Canada.
Waldron KC
No affiliation info available

MeSH

AnimalsChromatography, High Pressure LiquidElectrophoresis, CapillaryEquipment DesignHumansIndicators and ReagentsOnline SystemsPeptidesPharmaceutical PreparationsProteinsSensitivity and Specificity

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

11315155

Citation

Bonneil, E, and K C. Waldron. "On-line Solid-phase Preconcentration for Sensitivity Enhancement in Capillary Electrophoresis." Journal of Capillary Electrophoresis and Microchip Technology, vol. 6, no. 3-4, 1999, pp. 61-73.
Bonneil E, Waldron KC. On-line solid-phase preconcentration for sensitivity enhancement in capillary electrophoresis. J Capill Electrophor Microchip Technol. 1999;6(3-4):61-73.
Bonneil, E., & Waldron, K. C. (1999). On-line solid-phase preconcentration for sensitivity enhancement in capillary electrophoresis. Journal of Capillary Electrophoresis and Microchip Technology, 6(3-4), 61-73.
Bonneil E, Waldron KC. On-line Solid-phase Preconcentration for Sensitivity Enhancement in Capillary Electrophoresis. J Capill Electrophor Microchip Technol. 1999 May-Aug;6(3-4):61-73. PubMed PMID: 11315155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line solid-phase preconcentration for sensitivity enhancement in capillary electrophoresis. AU - Bonneil,E, AU - Waldron,K C, PY - 2001/4/21/pubmed PY - 2001/8/10/medline PY - 2001/4/21/entrez SP - 61 EP - 73 JF - Journal of capillary electrophoresis and microchip technology JO - J Capill Electrophor Microchip Technol VL - 6 IS - 3-4 N2 - Capillary electrophoresis is attractive for the analysis of biological samples because only a few nanoliters of sample need to be injected. Indeed, optimal resolution is achieved when the injected volume is 1% or less of the total capillary volume. Unfortunately, this advantage leads to severe detection limitations compounded by the fact that many analytes in a biological sample are present at very low concentrations. To overcome the detection sensitivity limitations of CE, nonspecific, on-line preconcentration has been employed in a variety of applications. This technique is based on inserting a small quantity of reversed-phase material (e.g., C18 particles or membrane) near the inlet of the CE capillary for sample enrichment by solid-phase extraction. A detachable cartridge containing the solid phase is relatively simple to construct and permits the injection of large sample volumes (1-100 microL) into the capillary. Elution of adsorbed analyte in less than 100 nL of solvent permits a 100-1000 fold improvement in the effective concentration limit of detection, depending on the hydrophobicity of the compound. Detection of analytes present in complex mixtures at concentrations as low as 20 amol/microL has been reported when using an on-line preconcentration device in conjunction with CE-mass spectrometry. In this review, the potential merits of the technique are described and some examples of direct analysis of biological samples without rigorous off-line pretreatment are given. SN - 1946-4940 UR - https://www.unboundmedicine.com/medline/citation/11315155/On_line_solid_phase_preconcentration_for_sensitivity_enhancement_in_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -