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Effect of randomization of mixtures of butter oil and vegetable oil on absorption and lipid metabolism in rats.
Eur J Nutr. 2001 Feb; 40(1):1-9.EJ

Abstract

BACKGROUND

The nutritional effect of the regiospecific distribution of fatty acids in edible fats is currently discussed due to an increased use of interesterification of fats for human consumption. However, disagreeing results have been reported which may be due to the varying composition of the dietary fats compared. Data on the fate of such lipids beyond the bloodstream is rather scarce and animal model studies are needed.

AIM OF THE STUDY

To compare the metabolism of butter oil and mixtures of butter and rapeseed oil, native or randomized, in a rat model. The regiospecific fatty acid distribution present in dietary fats was followed through absorption, chylomicron formation, and deposition in adipose tissue and in different liver lipids (triacylglycerols, phosholipids, and cholesterol esters).

METHODS

Rats were fed for 6 weeks from weaning either butter oil (BO), a butteroil-rapeseed oil mixture 65:35 w/w (BR) or a randomized mixture of BR (tBR). Half of the animals were used for organ analysis, the rest for a postprandial study with the same fats and isolation of chylomicrons. The regiospecific distribution of the fatty acids present in the dietary fats was followed during metabolism by analyses of chylomicrons, depot fat and liver lipids, using regiospecific cleavage followed by TLC separation and quantification by GC.

RESULTS

Randomization of edible fat mixtures leading to equal distribution of fatty acids between TG positions is directly reflected in the composition of chylomicrons. During clearing by lipoprotein lipase this positional distribution is abolished and the regiospecific composition of triacylglycerols in adipose tissue is completely identical for BR and tBR. Chylomicron remnants, which are taken up by the liver, are correspondingly fully degraded to free fatty acids by hepatic lipase, and distribution of fatty acids in liver triacylglycerols, phospholipids and cholesterol esters are identical for the groups fed either BR or tBR. The group fed BO with a low content of linoleic acid is on the borderline of essential fatty acid-deficiency.

CONCLUSION

Randomization (interesterification) of butter oil with rapeseed oil (65:35 w/w) for use as edible fat did not have any impact on the fatty acid composition beyond the chylomicron step when compared to the native mixture.

Authors+Show Affiliations

Department of Biochemistry and Nutrition, Building 224, Technical University of Denmark, 2800 Lyngby, Denmark. hoelmer@mimer.be.dtu.dkNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11315499

Citation

Becker, C, et al. "Effect of Randomization of Mixtures of Butter Oil and Vegetable Oil On Absorption and Lipid Metabolism in Rats." European Journal of Nutrition, vol. 40, no. 1, 2001, pp. 1-9.
Becker C, Lund P, Hølmer G. Effect of randomization of mixtures of butter oil and vegetable oil on absorption and lipid metabolism in rats. Eur J Nutr. 2001;40(1):1-9.
Becker, C., Lund, P., & Hølmer, G. (2001). Effect of randomization of mixtures of butter oil and vegetable oil on absorption and lipid metabolism in rats. European Journal of Nutrition, 40(1), 1-9.
Becker C, Lund P, Hølmer G. Effect of Randomization of Mixtures of Butter Oil and Vegetable Oil On Absorption and Lipid Metabolism in Rats. Eur J Nutr. 2001;40(1):1-9. PubMed PMID: 11315499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of randomization of mixtures of butter oil and vegetable oil on absorption and lipid metabolism in rats. AU - Becker,C, AU - Lund,P, AU - Hølmer,G, PY - 2001/4/24/pubmed PY - 2001/5/18/medline PY - 2001/4/24/entrez SP - 1 EP - 9 JF - European journal of nutrition JO - Eur J Nutr VL - 40 IS - 1 N2 - BACKGROUND: The nutritional effect of the regiospecific distribution of fatty acids in edible fats is currently discussed due to an increased use of interesterification of fats for human consumption. However, disagreeing results have been reported which may be due to the varying composition of the dietary fats compared. Data on the fate of such lipids beyond the bloodstream is rather scarce and animal model studies are needed. AIM OF THE STUDY: To compare the metabolism of butter oil and mixtures of butter and rapeseed oil, native or randomized, in a rat model. The regiospecific fatty acid distribution present in dietary fats was followed through absorption, chylomicron formation, and deposition in adipose tissue and in different liver lipids (triacylglycerols, phosholipids, and cholesterol esters). METHODS: Rats were fed for 6 weeks from weaning either butter oil (BO), a butteroil-rapeseed oil mixture 65:35 w/w (BR) or a randomized mixture of BR (tBR). Half of the animals were used for organ analysis, the rest for a postprandial study with the same fats and isolation of chylomicrons. The regiospecific distribution of the fatty acids present in the dietary fats was followed during metabolism by analyses of chylomicrons, depot fat and liver lipids, using regiospecific cleavage followed by TLC separation and quantification by GC. RESULTS: Randomization of edible fat mixtures leading to equal distribution of fatty acids between TG positions is directly reflected in the composition of chylomicrons. During clearing by lipoprotein lipase this positional distribution is abolished and the regiospecific composition of triacylglycerols in adipose tissue is completely identical for BR and tBR. Chylomicron remnants, which are taken up by the liver, are correspondingly fully degraded to free fatty acids by hepatic lipase, and distribution of fatty acids in liver triacylglycerols, phospholipids and cholesterol esters are identical for the groups fed either BR or tBR. The group fed BO with a low content of linoleic acid is on the borderline of essential fatty acid-deficiency. CONCLUSION: Randomization (interesterification) of butter oil with rapeseed oil (65:35 w/w) for use as edible fat did not have any impact on the fatty acid composition beyond the chylomicron step when compared to the native mixture. SN - 1436-6207 UR - https://www.unboundmedicine.com/medline/citation/11315499/Effect_of_randomization_of_mixtures_of_butter_oil_and_vegetable_oil_on_absorption_and_lipid_metabolism_in_rats_ L2 - https://dx.doi.org/10.1007/pl00007380 DB - PRIME DP - Unbound Medicine ER -