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Extrinsic neural innervation mediates absorption of water and electrolytes in canine proximal colon in vivo.
J Surg Res. 2001 May 01; 97(1):76-80.JS

Abstract

INTRODUCTION

Extrinsic innervation mediates a proabsorptive effect in small intestine. Our aim was to determine whether extrinsic neural input modulates similar effects in the proximal colon in vivo.

METHODS

Ten adult dogs underwent enteric isolation of a 50-cm proximal colon loop; five each were randomized to undergo extrinsic denervation (Ext Den) of the isolated colonic segment or to serve as neurally innervated controls. After recovery, a 38 degrees C electrolyte solution (Na(+) 125 meq/L, K(+) 9 meq/L, Cl(-) 75 meq/L, HC03(-) 65 meq/L) was infused at 4 ml/min into the segment. Effluent was collected in 30-min intervals for 2 h after achieving steady state (determined by 14C nonabsorbable marker recovery); four studies were conducted at 1 and 12 weeks postoperatively. Net flux of H20, Na(+), K(+), and Cl(-) was determined. Colon morphometry was evaluated at 0 and 14 weeks. Data are presented as mean +/- SEM. Unpaired t test was applied for comparisons.

RESULTS

Net absorptive flux of H20 (microL/min/cm) was decreased in Ext Den vs controls at 1 week (4.40 +/- 0.63 vs 7.92 +/- 0.92, P = 0.03) but was not different at 12 weeks (4.70 +/- 1.20 vs 5.97 +/- 0.69; P > 0.05). Na(+) and Cl(-) followed the trends in H20 absorption (P < or = 0.05). Crypt depth (microm) decreased in controls at 14 weeks vs 0 week (915 +/- 20 vs 740 +/- 07, P = 0.01) but remained unchanged in Ext Den.

CONCLUSIONS

Loss of extrinsic neural input decreases colonic absorption. This observation suggests that extrinsic neural innervation provides net proabsorptive mechanisms for absorption of water and electrolytes in the proximal canine colon.

Authors+Show Affiliations

Gastroenterologic Research Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11319884

Citation

Kendrick, M L., et al. "Extrinsic Neural Innervation Mediates Absorption of Water and Electrolytes in Canine Proximal Colon in Vivo." The Journal of Surgical Research, vol. 97, no. 1, 2001, pp. 76-80.
Kendrick ML, Meile T, Zyromski NJ, et al. Extrinsic neural innervation mediates absorption of water and electrolytes in canine proximal colon in vivo. J Surg Res. 2001;97(1):76-80.
Kendrick, M. L., Meile, T., Zyromski, N. J., Tanaka, T., & Sarr, M. G. (2001). Extrinsic neural innervation mediates absorption of water and electrolytes in canine proximal colon in vivo. The Journal of Surgical Research, 97(1), 76-80.
Kendrick ML, et al. Extrinsic Neural Innervation Mediates Absorption of Water and Electrolytes in Canine Proximal Colon in Vivo. J Surg Res. 2001 May 1;97(1):76-80. PubMed PMID: 11319884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extrinsic neural innervation mediates absorption of water and electrolytes in canine proximal colon in vivo. AU - Kendrick,M L, AU - Meile,T, AU - Zyromski,N J, AU - Tanaka,T, AU - Sarr,M G, PY - 2001/4/26/pubmed PY - 2001/6/15/medline PY - 2001/4/26/entrez SP - 76 EP - 80 JF - The Journal of surgical research JO - J. Surg. Res. VL - 97 IS - 1 N2 - INTRODUCTION: Extrinsic innervation mediates a proabsorptive effect in small intestine. Our aim was to determine whether extrinsic neural input modulates similar effects in the proximal colon in vivo. METHODS: Ten adult dogs underwent enteric isolation of a 50-cm proximal colon loop; five each were randomized to undergo extrinsic denervation (Ext Den) of the isolated colonic segment or to serve as neurally innervated controls. After recovery, a 38 degrees C electrolyte solution (Na(+) 125 meq/L, K(+) 9 meq/L, Cl(-) 75 meq/L, HC03(-) 65 meq/L) was infused at 4 ml/min into the segment. Effluent was collected in 30-min intervals for 2 h after achieving steady state (determined by 14C nonabsorbable marker recovery); four studies were conducted at 1 and 12 weeks postoperatively. Net flux of H20, Na(+), K(+), and Cl(-) was determined. Colon morphometry was evaluated at 0 and 14 weeks. Data are presented as mean +/- SEM. Unpaired t test was applied for comparisons. RESULTS: Net absorptive flux of H20 (microL/min/cm) was decreased in Ext Den vs controls at 1 week (4.40 +/- 0.63 vs 7.92 +/- 0.92, P = 0.03) but was not different at 12 weeks (4.70 +/- 1.20 vs 5.97 +/- 0.69; P > 0.05). Na(+) and Cl(-) followed the trends in H20 absorption (P < or = 0.05). Crypt depth (microm) decreased in controls at 14 weeks vs 0 week (915 +/- 20 vs 740 +/- 07, P = 0.01) but remained unchanged in Ext Den. CONCLUSIONS: Loss of extrinsic neural input decreases colonic absorption. This observation suggests that extrinsic neural innervation provides net proabsorptive mechanisms for absorption of water and electrolytes in the proximal canine colon. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/11319884/Extrinsic_neural_innervation_mediates_absorption_of_water_and_electrolytes_in_canine_proximal_colon_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(01)96115-3 DB - PRIME DP - Unbound Medicine ER -