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Inhibitory effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) propane hydrochloride on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes.
Acta Pharmacol Sin. 2000 Apr; 21(4):301-5.AP

Abstract

AIM

To study the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenyl-ethylamino) propane hydrochloride (DDPH) on action potential (AP), inward rectifier K+ current (IK1), and delayed rectifier K+ current (IK) in isolated guinea pig ventricular myocytes.

METHODS

Whole cell patch-clamp recording techniques.

RESULTS

DDPH 0.1-100 mumol.L-1 decreased 50% duration of action potential (APD50) concentration-dependently. APD50 was shortened from (493 +/- 58) to (262 +/- 38) ms (n = 7 cells from 5 guinea pigs, P < 0.01) by DDPH 10 mumol.L-1. However, 90% duration of action potential (APD90) was increased by DDPH (> 1 mumol.L-1). At high concentration (> 10 mumol.L-1) DDPH decreased resting membrane potential (RP) and amplitude of action potential (APA). DDPH inhibited tail current of IK (IK.tail) concentration-dependently, 46% at 10 mumol.L-1 and 78% at 100 mumol.L-1. EC50 for DDPH inhibiting IK was 13.3 (11.6-16.7) mumol.L-1. DDPH also blocked IK1. DDPH at high concentration (> 10 mumol.L-1) shifted the reverse potential of IK1 positively. All the effects of DDPH were reversible after washout.

CONCLUSION

DDPH blocked both IK1 and IK current in guinea pig ventricular myocytes.

Authors+Show Affiliations

Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031, China. nolannz@sunm.shcnc.ac.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11324454

Citation

Zhong, N, et al. "Inhibitory Effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) Propane Hydrochloride On Inward Rectifier and Delayed Rectifier K+ Currents in Guinea Pig Ventricular Myocytes." Acta Pharmacologica Sinica, vol. 21, no. 4, 2000, pp. 301-5.
Zhong N, Fang QZ, Zhang Y, et al. Inhibitory effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) propane hydrochloride on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. Acta Pharmacol Sin. 2000;21(4):301-5.
Zhong, N., Fang, Q. Z., Zhang, Y., Qian, J. Q., & Zhou, Z. N. (2000). Inhibitory effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) propane hydrochloride on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. Acta Pharmacologica Sinica, 21(4), 301-5.
Zhong N, et al. Inhibitory Effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) Propane Hydrochloride On Inward Rectifier and Delayed Rectifier K+ Currents in Guinea Pig Ventricular Myocytes. Acta Pharmacol Sin. 2000;21(4):301-5. PubMed PMID: 11324454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenyl-ethylamino) propane hydrochloride on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. AU - Zhong,N, AU - Fang,Q Z, AU - Zhang,Y, AU - Qian,J Q, AU - Zhou,Z N, PY - 2001/4/28/pubmed PY - 2001/6/22/medline PY - 2001/4/28/entrez SP - 301 EP - 5 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 21 IS - 4 N2 - AIM: To study the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenyl-ethylamino) propane hydrochloride (DDPH) on action potential (AP), inward rectifier K+ current (IK1), and delayed rectifier K+ current (IK) in isolated guinea pig ventricular myocytes. METHODS: Whole cell patch-clamp recording techniques. RESULTS: DDPH 0.1-100 mumol.L-1 decreased 50% duration of action potential (APD50) concentration-dependently. APD50 was shortened from (493 +/- 58) to (262 +/- 38) ms (n = 7 cells from 5 guinea pigs, P < 0.01) by DDPH 10 mumol.L-1. However, 90% duration of action potential (APD90) was increased by DDPH (> 1 mumol.L-1). At high concentration (> 10 mumol.L-1) DDPH decreased resting membrane potential (RP) and amplitude of action potential (APA). DDPH inhibited tail current of IK (IK.tail) concentration-dependently, 46% at 10 mumol.L-1 and 78% at 100 mumol.L-1. EC50 for DDPH inhibiting IK was 13.3 (11.6-16.7) mumol.L-1. DDPH also blocked IK1. DDPH at high concentration (> 10 mumol.L-1) shifted the reverse potential of IK1 positively. All the effects of DDPH were reversible after washout. CONCLUSION: DDPH blocked both IK1 and IK current in guinea pig ventricular myocytes. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/11324454/Inhibitory_effect_of_1__2_6_dimethylphenoxy__2__3_4_dimethoxyphenyl_ethylamino__propane_hydrochloride_on_inward_rectifier_and_delayed_rectifier_K+_currents_in_guinea_pig_ventricular_myocytes_ L2 - http://www.chinaphar.com/1671-4083/21/301.pdf DB - PRIME DP - Unbound Medicine ER -