Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection.
Cell Biochem Biophys. 2000; 33(3):323-37.CB

Abstract

Interest in the use of capillary electrophoresis (CE) as a tool for protein separations continues to grow. Additionally, laser-induced fluorescence (LIF) detection schemes promise ultrasensitive detection of small quantities of these important biomolecules following their separation. In most cases, LIF detection of proteins necessitates their prior derivatization with a fluorescent label molecule. To minimize the amount of additional sample handling and time associated with such labeling procedures, not to mention the sometimes-stringent pH and temperature controls they require, noncovalent labeling is presented as a viable alternative. This review article considers established methods for noncovalent labeling of proteins for their subsequent analysis by CE-LIF. Label molecules suitable for excitation and emission in the ultraviolet, visible, and near-infrared regions of the spectrum are enumerated for a variety of protein analytes.

Authors+Show Affiliations

Colyer C

Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, USA. colyerc@wfu.edu

MeSH

AnimalsElectrophoresis, CapillaryFluorescenceHumansLasersProteins

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Review

Language

eng

PubMed ID

11325049

Citation

Colyer, C. "Noncovalent Labeling of Proteins in Capillary Electrophoresis With Laser-induced Fluorescence Detection." Cell Biochemistry and Biophysics, vol. 33, no. 3, 2000, pp. 323-37.

Colyer C. Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection. Cell Biochem Biophys. 2000;33(3):323-37.

Colyer, C. (2000). Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection. Cell Biochemistry and Biophysics, 33(3), 323-37.

Colyer C. Noncovalent Labeling of Proteins in Capillary Electrophoresis With Laser-induced Fluorescence Detection. Cell Biochem Biophys. 2000;33(3):323-37. PubMed PMID: 11325049.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection. A1 - Colyer,C, PY - 2001/4/28/pubmed PY - 2001/6/15/medline PY - 2001/4/28/entrez SP - 323 EP - 37 JF - Cell biochemistry and biophysics JO - Cell Biochem Biophys VL - 33 IS - 3 N2 - Interest in the use of capillary electrophoresis (CE) as a tool for protein separations continues to grow. Additionally, laser-induced fluorescence (LIF) detection schemes promise ultrasensitive detection of small quantities of these important biomolecules following their separation. In most cases, LIF detection of proteins necessitates their prior derivatization with a fluorescent label molecule. To minimize the amount of additional sample handling and time associated with such labeling procedures, not to mention the sometimes-stringent pH and temperature controls they require, noncovalent labeling is presented as a viable alternative. This review article considers established methods for noncovalent labeling of proteins for their subsequent analysis by CE-LIF. Label molecules suitable for excitation and emission in the ultraviolet, visible, and near-infrared regions of the spectrum are enumerated for a variety of protein analytes. SN - 1085-9195 UR - https://www.unboundmedicine.com/medline/citation/11325049/Noncovalent_labeling_of_proteins_in_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -

Tags

Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection.Cell Biochem Biophys. 2000; 33(3):323-37.CB